2013
Differentiation of Lactobacillus Species by ARDRA
KŠICOVÁ, Kateřina, Marta DUŠKOVÁ a Renata KARPÍŠKOVÁZákladní údaje
Originální název
Differentiation of Lactobacillus Species by ARDRA
Autoři
KŠICOVÁ, Kateřina (203 Česká republika, domácí), Marta DUŠKOVÁ (203 Česká republika, garant) a Renata KARPÍŠKOVÁ (203 Česká republika)
Vydání
Czech Journal of Food Science, 2013, 1212-1800
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 0.741
Kód RIV
RIV/00216224:14310/13:00069157
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000318259400011
Klíčová slova anglicky
: restriction analysis of the 16S rDNA gene; aluI; MspI; in silico fragmentation; species identification
Změněno: 28. 4. 2014 14:57, Ing. Zdeňka Rašková
Anotace
V originále
The Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as starter cultures in food fermentation processes. The species-specific identification by traditional biochemical methods is time consuming and not always fully effective. Therefore, more efficient techniques are searched for. We focused on rapid identification of Lactobacillus isolates from different habitats. Forty-nine collection strains and isolates belonging to the genus Lactobacillus were discriminated. ARDRA was carried out with two restriction endonucleases. For the comparison of similarity, the Jaccard coefficient and clustering by the unweighted pair group method with arithmetic averages (UPGMA) were used. The percentages of similarity between profiles varied from 22% to 100% (aluI) and from 27% to 100% (MspI). This method proved applicable to the differentiation of 10 species.
Návaznosti
MSM0021622415, záměr |
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