Dirofilaria repens microfilariae in Aedes vexans mosquitoes in
Slovakia

J 2013

Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia

BOCKOVÁ, Eva, Ivo RUDOLF, Alica KOČIŠOVÁ, Lenka BETÁŠOVÁ, Kristýna VENCLÍKOVÁ et. al.

Základní údaje

Originální název

Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia

Autoři

BOCKOVÁ, Eva (703 Slovensko, garant), Ivo RUDOLF (203 Česká republika, domácí), Alica KOČIŠOVÁ (703 Slovensko), Lenka BETÁŠOVÁ (703 Slovensko), Kristýna VENCLÍKOVÁ (203 Česká republika, domácí), Jan MENDEL (203 Česká republika) a Zdeněk HUBÁLEK (203 Česká republika, domácí)

Vydání

Parasitology Research, 2013, 0932-0113

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.327

Kód RIV

RIV/00216224:14310/13:00069247

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s00436-013-3526-9

UT WoS

000324326500010

Klíčová slova anglicky

Aedes vexans; microfilariae; Dirofilaria repens; Dirofilaria immitis; canine dirofilariosis; Slovakia

Štítky

AKR, rivok, ZR

Příznaky

Recenzováno

Změněno: 28. 4. 2014 15:29, Ing. Zdeňka Rašková

Anotace

V originále

t In this study, we screened field-caught mosquitoes for presence of Dirofilaria spp. by using a polymerase chain reaction (PCR) assay. Potential occurrence of Dirofilaria repens and Dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (Aedes vexans, Aedes cinereus, Aedes rossicus, Culex pipiens, Culiseta annulata, Ochlerotatus sticticus, Ochlerotatus cantans and Ochlerotatus caspius) collected from five locations in two districts (Kosice and Trebisov) of Eastern Slovakia, endemic region of canine dirofilariasis. Collection of mosquitoes was performed between May and August 2012 in premises known to be inhabited by Dirofilaria-infected dogs. PCR assays were performed on 72 pools, each pool containing 50 mosquitoes of the same species, collected on the same location. Each pool was examined separately for the presence of D. immitis and D. repens, respectively. A positive finding ofD. repenswas recorded in one pool of A. vexans mosquitoes collected in Košické Olšany village. Minimum infection rate in A. vexans was 1:1,750, i.e. 0.57 per 1,000 mosquitoes. The identity of D. repens was confirmed by direct sequencing of PCR product which has shown 100 % homology with sequence attributed to D. repens (GenBank accession number AJ271614). This study represents the first molecular evidence of D. repensmicrofilariae in mosquitoes in Slovakia and highlights a need for better surveillance of zoonotic dirofilariasis in central Europe.

Citovat

BOCKOVÁ, Eva, Ivo RUDOLF, Alica KOČIŠOVÁ, Lenka BETÁŠOVÁ, Kristýna VENCLÍKOVÁ, Jan MENDEL a Zdeněk HUBÁLEK. Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia. Parasitology Research. 2013, roč. 112, č. 10, s. 3465-3470. ISSN 0932-0113. Dostupné z: https://dx.doi.org/10.1007/s00436-013-3526-9.

@article{1121893,
   author = {Bocková, Eva and Rudolf, Ivo and Kočišová, Alica and Betášová, Lenka and Venclíková, Kristýna and Mendel, Jan and Hubálek, Zdeněk},
   article_number = {10},
   doi = {http://dx.doi.org/10.1007/s00436-013-3526-9},
   keywords = {Aedes vexans; microfilariae; Dirofilaria repens; Dirofilaria immitis; canine dirofilariosis; Slovakia},
   language = {eng},
   issn = {0932-0113},
   journal = {Parasitology Research},
   title = {Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia},
   volume = {112},
   year = {2013}
}

TY  - JOUR
ID  - 1121893
AU  - Bocková, Eva - Rudolf, Ivo - Kočišová, Alica - Betášová, Lenka - Venclíková, Kristýna - Mendel, Jan - Hubálek, Zdeněk
PY  - 2013
TI  - Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia
JF  - Parasitology Research
VL  - 112
IS  - 10
SP  - 3465-3470
EP  - 3465-3470
SN  - 09320113
KW  - Aedes vexans
KW  - microfilariae
KW  - Dirofilaria repens
KW  - Dirofilaria immitis
KW  - canine dirofilariosis
KW  - Slovakia
N2  - t In this study, we screened field-caught mosquitoes for presence of Dirofilaria spp. by using a polymerase chain reaction (PCR) assay. Potential occurrence of Dirofilaria repens and Dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (Aedes vexans, Aedes cinereus, Aedes rossicus, Culex pipiens, Culiseta annulata, Ochlerotatus sticticus, Ochlerotatus cantans and Ochlerotatus caspius) collected from five locations in two districts (Kosice and Trebisov) of Eastern Slovakia, endemic region of canine dirofilariasis. Collection of mosquitoes was performed between May and August 2012 in premises known to be inhabited by Dirofilaria-infected dogs. PCR assays were performed on 72 pools, each pool containing 50 mosquitoes of the same species, collected on the same location. Each pool was examined separately for the presence of D. immitis and D. repens, respectively. A positive finding ofD. repenswas recorded in one pool of A. vexans mosquitoes collected in Košické Olšany village. Minimum infection rate in A. vexans was 1:1,750, i.e. 0.57 per 1,000 mosquitoes. The identity of D. repens was confirmed by direct sequencing of PCR product which has shown 100 % homology with sequence attributed to D. repens (GenBank accession number AJ271614). This study represents the first molecular evidence of D. repensmicrofilariae in mosquitoes in Slovakia and highlights a need for better surveillance of zoonotic dirofilariasis in central Europe.
ER  -

BOCKOVÁ, Eva, Ivo RUDOLF, Alica KOČIŠOVÁ, Lenka BETÁŠOVÁ, Kristýna VENCLÍKOVÁ, Jan MENDEL a Zdeněk HUBÁLEK. Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia. \textit{Parasitology Research}. 2013, roč.~112, č.~10, s.~3465-3470. ISSN~0932-0113. Dostupné z: https://dx.doi.org/10.1007/s00436-013-3526-9.

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