Role HIF-1alpha in cardiomyogenesis

a 2013

Role HIF-1alpha in cardiomyogenesis

NAVRÁTILOVÁ, Jana, Dominika SÝKOROVÁ, Lucia BINÓ, Hana KOLÁŘOVÁ, Pavel KARAS et. al.

Základní údaje

Originální název

Role HIF-1alpha in cardiomyogenesis

Autoři

NAVRÁTILOVÁ, Jana, Dominika SÝKOROVÁ, Lucia BINÓ, Hana KOLÁŘOVÁ, Pavel KARAS, Lukáš KOHÚT, Jiří PACHERNÍK a Lukáš KUBALA

Vydání

ISHR XXI World Congress, 2013

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Změněno: 16. 9. 2013 13:48, Mgr. Jana Kudová, Ph.D.

Anotace

V originále

Cardiomyogenesis, a cardiac cell formation, includes differentiation, proliferation and migration of early cardiomyocytes. In vitro, mature cardiomyocytes are characterized by spontaneous beating and expression of cardiac-specific genes. Interestingly, under pathological conditions such as heart ischemia an activation of fetal gene expression connected with reactivation of cardiomyogenic process is reported. This effect is suggested to help the cardiac tissues to adapt to stress conditions. Cardiomyogenesis is regulated by a large number of factors including oxygen availability. Hypoxic conditions, which are characteristic for early embryonic development, lead to stabilization of hypoxia inducible factor 1 alpha. However, role of HIF-1alpha in regulation of cardiomyocyte differentiation is not well understood. With aim to better understand the role of HIF-1alpha within cardiomyocyte development and reprograming, cardiomyogenesis in wild type and HIF-1alpha depleted murine embryonic stem cells was analyzed in vitro. The level of expression of individual cardiospecific genes was significantly lower in HIF-1alpha deficient cell line compared to wild type cells after induction of cardiomyogenesis in vitro. This suggests lower number of progenitor cells to enter cardiomyogenesis in these HIF-1alpha deficient cells. However, despite this effect, cells which have already entered the cardiomyogenic process are similar in both wild type and HIF-1alpha deficient line. They both start to beat at the same time after the onset of differentiation and their beating characteristic is comparable. Moreover, they have a similar morphology. Thus, although HIF-1alpha is important for induction of development of cardiomyogenic progenitors HIF-1alpha had no significant effect on cardiomyocyte maturation in in vitro culture.

Citovat

NAVRÁTILOVÁ, Jana, Dominika SÝKOROVÁ, Lucia BINÓ, Hana KOLÁŘOVÁ, Pavel KARAS, Lukáš KOHÚT, Jiří PACHERNÍK a Lukáš KUBALA. Role HIF-1alpha in cardiomyogenesis. In ISHR XXI World Congress. 2013.

@proceedings{1122532,
   author = {Navrátilová, Jana and Sýkorová, Dominika and Binó, Lucia and Kolářová, Hana and Karas, Pavel and Kohút, Lukáš and Pacherník, Jiří and Kubala, Lukáš},
   booktitle = {ISHR XXI World Congress},
   title = {Role HIF-1alpha in cardiomyogenesis},
   year = {2013}
}

TY  - CONF
ID  - 1122532
AU  - Navrátilová, Jana - Sýkorová, Dominika - Binó, Lucia - Kolářová, Hana - Karas, Pavel - Kohút, Lukáš - Pacherník, Jiří - Kubala, Lukáš
PY  - 2013
TI  - Role HIF-1alpha in cardiomyogenesis
N2  - Cardiomyogenesis, a cardiac cell formation, includes differentiation, proliferation and migration of early cardiomyocytes. In vitro, mature cardiomyocytes are characterized by spontaneous beating and expression of cardiac-specific genes. Interestingly, under pathological conditions such as heart ischemia an activation of fetal gene expression connected with reactivation of cardiomyogenic process is reported. This effect is suggested to help the cardiac tissues to adapt to stress conditions. Cardiomyogenesis is regulated by a large number of factors including oxygen availability. Hypoxic conditions, which are characteristic for early embryonic development, lead to stabilization of hypoxia inducible factor 1 alpha. However, role of HIF-1alpha in regulation of cardiomyocyte differentiation is not well understood. With aim to better understand the role of HIF-1alpha within cardiomyocyte development and reprograming, cardiomyogenesis in wild type and HIF-1alpha depleted murine embryonic stem cells was analyzed in vitro. The level of expression of individual cardiospecific genes was significantly lower in HIF-1alpha deficient cell line compared to wild type cells after induction of cardiomyogenesis in vitro. This suggests lower number of progenitor cells to enter cardiomyogenesis in these HIF-1alpha deficient cells. However, despite this effect, cells which have already entered the cardiomyogenic process are similar in both wild type and HIF-1alpha deficient line. They both start to beat at the same time after the onset of differentiation and their beating characteristic is comparable. Moreover, they have a similar morphology. Thus, although HIF-1alpha is important for induction of development of cardiomyogenic progenitors HIF-1alpha had no significant effect on cardiomyocyte maturation in in vitro culture.
ER  -

NAVRÁTILOVÁ, Jana, Dominika SÝKOROVÁ, Lucia BINÓ, Hana KOLÁŘOVÁ, Pavel KARAS, Lukáš KOHÚT, Jiří PACHERNÍK a Lukáš KUBALA. Role HIF-1alpha in cardiomyogenesis. In \textit{ISHR XXI World Congress}. 2013.

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