Evaluation of the strain identity between isolates from caries
lesions and root canals in early childhood caries cases

J 2013

Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases

NOVÁKOVÁ, Dana, Pavel ŠVEC, Martina KUKLETOVÁ, Lenka ŽÁČKOVÁ, Ivo SEDLÁČEK et. al.

Základní údaje

Originální název

Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases

Autoři

NOVÁKOVÁ, Dana (203 Česká republika, garant, domácí), Pavel ŠVEC (203 Česká republika, domácí), Martina KUKLETOVÁ (203 Česká republika, domácí), Lenka ŽÁČKOVÁ (203 Česká republika) a Ivo SEDLÁČEK (203 Česká republika, domácí)

Vydání

Folia Microbiologica, AV ČR, Institute of Microbiology, 2013, 0015-5632

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.145

Kód RIV

RIV/00216224:14310/13:00069595

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s12223-013-0254-6

UT WoS

000324835100029

Klíčová slova anglicky

biotyping; ribotyping; rep-PCR; caries

Štítky

AKR, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 4. 2014 09:45, Ing. Zdeňka Rašková

Anotace

V originále

Early childhood caries (ECC) has become a serious medical problem worldwide in the last decade. Bacterial microflora of the dental plaque and oral cavity is considered an important factor in the formation and progression of dental caries. The aim of this study was strain typing and comparison of bacterial isolates retrieved from caries lesions and root canal contents of the same teeth. In total, 18 pairs of presumptive streptococci and lactobacilli retrieved from dental caries and root canals isolated from ECC-affected children, were selected on the basis of biotyping results and rep-PCR fingerprinting with (GTG)5 primer. Strain typing was further done using the RiboPrinter microbial characterization system (DuPont Qualicon). The automated ribotyping determined 14 pairs of the strains (77.8 %) to be identical. The results obtained confirmed that identical bacterial strains colonized both the decayed dental surface and the necrotic content of the dental pulp cavity during the cariogenesis. Our finding supports the assumption that bacteria could penetrate through the damaged dental surface to the inner parts of the teeth.

Návaznosti

EE2.3.20.0183, projekt VaV

Název: Centrum experimentální biomedicíny

Citovat

NOVÁKOVÁ, Dana, Pavel ŠVEC, Martina KUKLETOVÁ, Lenka ŽÁČKOVÁ a Ivo SEDLÁČEK. Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases. Folia Microbiologica. AV ČR, Institute of Microbiology, 2013, roč. 58, č. 6, s. 649-656. ISSN 0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-013-0254-6.

@article{1124503,
   author = {Nováková, Dana and Švec, Pavel and Kukletová, Martina and Žáčková, Lenka and Sedláček, Ivo},
   article_number = {6},
   doi = {http://dx.doi.org/10.1007/s12223-013-0254-6},
   keywords = {biotyping; ribotyping; rep-PCR; caries},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases},
   volume = {58},
   year = {2013}
}

TY  - JOUR
ID  - 1124503
AU  - Nováková, Dana - Švec, Pavel - Kukletová, Martina - Žáčková, Lenka - Sedláček, Ivo
PY  - 2013
TI  - Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases
JF  - Folia Microbiologica
VL  - 58
IS  - 6
SP  - 649-656
EP  - 649-656
PB  - AV ČR, Institute of Microbiology
SN  - 00155632
KW  - biotyping
KW  - ribotyping
KW  - rep-PCR
KW  - caries
N2  - Early childhood caries (ECC) has become a serious medical problem worldwide in the last decade. Bacterial microflora of the dental plaque and oral cavity is considered an important factor in the formation and progression of dental caries. The aim of this study was strain typing and comparison of bacterial isolates retrieved from caries lesions and root canal contents of the same teeth. In total, 18 pairs of presumptive streptococci and lactobacilli retrieved from dental caries and root canals isolated from ECC-affected children, were selected on the basis of biotyping results and rep-PCR fingerprinting with (GTG)5 primer. Strain typing was further done using the RiboPrinter microbial characterization system (DuPont Qualicon). The automated ribotyping determined 14 pairs of the strains (77.8 %) to be identical. The results obtained confirmed that identical bacterial strains colonized both the decayed dental surface and the necrotic content of the dental pulp cavity during the cariogenesis. Our finding supports the assumption that bacteria could penetrate through the damaged dental surface to the inner parts of the teeth.
ER  -

NOVÁKOVÁ, Dana, Pavel ŠVEC, Martina KUKLETOVÁ, Lenka ŽÁČKOVÁ a Ivo SEDLÁČEK. Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases. \textit{Folia Microbiologica}. AV ČR, Institute of Microbiology, 2013, roč.~58, č.~6, s.~649-656. ISSN~0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-013-0254-6.

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