NOVÁKOVÁ, Dana, Pavel ŠVEC, Martina KUKLETOVÁ, Lenka ŽÁČKOVÁ a Ivo SEDLÁČEK. Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases. Folia Microbiologica. AV ČR, Institute of Microbiology, 2013, roč. 58, č. 6, s. 649-656. ISSN 0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-013-0254-6. |
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@article{1124503, author = {Nováková, Dana and Švec, Pavel and Kukletová, Martina and Žáčková, Lenka and Sedláček, Ivo}, article_number = {6}, doi = {http://dx.doi.org/10.1007/s12223-013-0254-6}, keywords = {biotyping; ribotyping; rep-PCR; caries}, language = {eng}, issn = {0015-5632}, journal = {Folia Microbiologica}, title = {Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases}, volume = {58}, year = {2013} }
TY - JOUR ID - 1124503 AU - Nováková, Dana - Švec, Pavel - Kukletová, Martina - Žáčková, Lenka - Sedláček, Ivo PY - 2013 TI - Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases JF - Folia Microbiologica VL - 58 IS - 6 SP - 649-656 EP - 649-656 PB - AV ČR, Institute of Microbiology SN - 00155632 KW - biotyping KW - ribotyping KW - rep-PCR KW - caries N2 - Early childhood caries (ECC) has become a serious medical problem worldwide in the last decade. Bacterial microflora of the dental plaque and oral cavity is considered an important factor in the formation and progression of dental caries. The aim of this study was strain typing and comparison of bacterial isolates retrieved from caries lesions and root canal contents of the same teeth. In total, 18 pairs of presumptive streptococci and lactobacilli retrieved from dental caries and root canals isolated from ECC-affected children, were selected on the basis of biotyping results and rep-PCR fingerprinting with (GTG)5 primer. Strain typing was further done using the RiboPrinter microbial characterization system (DuPont Qualicon). The automated ribotyping determined 14 pairs of the strains (77.8 %) to be identical. The results obtained confirmed that identical bacterial strains colonized both the decayed dental surface and the necrotic content of the dental pulp cavity during the cariogenesis. Our finding supports the assumption that bacteria could penetrate through the damaged dental surface to the inner parts of the teeth. ER -
NOVÁKOVÁ, Dana, Pavel ŠVEC, Martina KUKLETOVÁ, Lenka ŽÁČKOVÁ a Ivo SEDLÁČEK. Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases. \textit{Folia Microbiologica}. AV ČR, Institute of Microbiology, 2013, roč.~58, č.~6, s.~649-656. ISSN~0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-013-0254-6.
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