Characterization of Aeromonas popoffii isolates

a 2013

Characterization of Aeromonas popoffii isolates

SEDLÁČEK, Ivo, Ondrej ŠEDO, Tereza GELBÍČOVÁ, Andrea VÁVROVÁ PASCUTTI, Eva KROUPOVÁ et. al.

Základní údaje

Originální název

Characterization of Aeromonas popoffii isolates

Autoři

SEDLÁČEK, Ivo (203 Česká republika, garant, domácí), Ondrej ŠEDO (203 Česká republika, domácí), Tereza GELBÍČOVÁ (203 Česká republika, domácí), Andrea VÁVROVÁ PASCUTTI (203 Česká republika, domácí), Eva KROUPOVÁ (203 Česká republika, domácí), Michaela SEDLÁČKOVÁ (203 Česká republika, domácí) a Pavel ŠVEC (203 Česká republika, domácí)

Vydání

ICCC13, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/13:00069599

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Aeromonas popoffii; MALDI-TOF MS; ribotyping; phenotyping

Změněno: 29. 4. 2014 09:46, Ing. Zdeňka Rašková

Anotace

V originále

Forty eight A. popoffii-like isolates from water sources (e.g. river freshwater, water pond, underground water and stream water) sampled in Czech Republic and Slovakia during 2008-2012, were characterized by PvuII ribotyping (using RiboPrinter identification system), extensive biochemical testing (tube and plate tests, ENTEROtest 24, Biolog system) and MALDI-TOF MS analysis along with reference strains of A. hydrophila group. These strains were tentatively identified as A. popoffii on the base of the negative sucrose acidification, which is a typical trait of this species. MALDI-TOF MS showed the highest discriminatory power for presumptive A. popoffii isolates and this method seems to be valuable for species identification of A. hydrophila group. RiboPrinter demonstrated great heterogeneity of ribotyping patterns among A. popoffii strains but their identification is impracticable due to the absence of A. popoffii reference patterns in the RiboPrinter identification database. Four presumptive A. popoffii isolates (P3282, P4293, P4571 and P4573) displayed very distinct riboprint profiles. Their further investigation using 16S rRNA and cpn60 genes sequencing assigned these strains as Aeromonas veronii and Aeromonas sobria species.

Návaznosti

EE2.3.20.0183, projekt VaV

Název: Centrum experimentální biomedicíny

Citovat

SEDLÁČEK, Ivo, Ondrej ŠEDO, Tereza GELBÍČOVÁ, Andrea VÁVROVÁ PASCUTTI, Eva KROUPOVÁ, Michaela SEDLÁČKOVÁ a Pavel ŠVEC. Characterization of Aeromonas popoffii isolates. In ICCC13. 2013.

@proceedings{1124512,
   author = {Sedláček, Ivo and Šedo, Ondrej and Gelbíčová, Tereza and Vávrová Pascutti, Andrea and Kroupová, Eva and Sedláčková, Michaela and Švec, Pavel},
   booktitle = {ICCC13},
   keywords = {Aeromonas popoffii; MALDI-TOF MS; ribotyping; phenotyping},
   language = {eng},
   title = {Characterization of Aeromonas popoffii isolates},
   year = {2013}
}

TY  - CONF
ID  - 1124512
AU  - Sedláček, Ivo - Šedo, Ondrej - Gelbíčová, Tereza - Vávrová Pascutti, Andrea - Kroupová, Eva - Sedláčková, Michaela - Švec, Pavel
PY  - 2013
TI  - Characterization of Aeromonas popoffii isolates
KW  - Aeromonas popoffii
KW  - MALDI-TOF MS
KW  - ribotyping
KW  - phenotyping
N2  - Forty eight A. popoffii-like isolates from water sources (e.g. river freshwater, water pond, underground water and stream water) sampled in Czech Republic and Slovakia during 2008-2012, were characterized by PvuII ribotyping (using RiboPrinter identification system), extensive biochemical testing (tube and plate tests, ENTEROtest 24, Biolog system) and MALDI-TOF MS analysis along with reference strains of A. hydrophila group. These strains were tentatively identified as A. popoffii on the base of the negative sucrose acidification, which is a typical trait of this species. MALDI-TOF MS showed the highest discriminatory power for presumptive A. popoffii isolates and this method seems to be valuable for species identification of A. hydrophila group. RiboPrinter demonstrated great heterogeneity of ribotyping patterns among A. popoffii strains but their identification is impracticable due to the absence of A. popoffii reference patterns in the RiboPrinter identification database. Four presumptive A. popoffii isolates (P3282, P4293, P4571 and P4573) displayed very distinct riboprint profiles. Their further investigation using 16S rRNA and cpn60 genes sequencing assigned these strains as Aeromonas veronii and Aeromonas sobria species.
ER  -

SEDLÁČEK, Ivo, Ondrej ŠEDO, Tereza GELBÍČOVÁ, Andrea VÁVROVÁ PASCUTTI, Eva KROUPOVÁ, Michaela SEDLÁČKOVÁ a Pavel ŠVEC. Characterization of Aeromonas popoffii isolates. In \textit{ICCC13}. 2013.

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