Detailed Information on Publication Record
2013
Comparison of CDC and sequence-based molecular typing of syphilis treponemes: tpr and arp loci are variable in multiple samples from the same patient
MIKALOVÁ, Lenka, Petra POSPÍŠILOVÁ, Vladana WOZNICOVÁ, Ivana KUKLOVA, Hana ZAKOUCKA et. al.Basic information
Original name
Comparison of CDC and sequence-based molecular typing of syphilis treponemes: tpr and arp loci are variable in multiple samples from the same patient
Authors
MIKALOVÁ, Lenka (203 Czech Republic, belonging to the institution), Petra POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Vladana WOZNICOVÁ (203 Czech Republic, belonging to the institution), Ivana KUKLOVA (203 Czech Republic), Hana ZAKOUCKA (203 Czech Republic) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)
Edition
BMC Microbiology, LONDON, BioMed Central, 2013, 1471-2180
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.976
RIV identification code
RIV/00216224:14110/13:00065630
Organization unit
Faculty of Medicine
UT WoS
000322731800001
Keywords in English
Treponema pallidum; Syphilis; Molecular typing; Sequence-based typing; CDC typing; arp gene; tpr genes
Tags
International impact, Reviewed
Změněno: 4/11/2013 11:01, Soňa Böhmová
Abstract
V originále
Background: Molecular typing of syphilis-causing strains provides important epidemiologic data. We tested whether identified molecular subtypes were identical in PCR-positive parallel samples taken from the same patient at a same time. We also tested whether subtype prevalence differs in skin and blood samples. Results: Eighteen syphilis positive patients (showing both positive serology and PCR), with two PCR-typeable parallel samples taken at the same time, were tested with both CDC (Centers for Disease Control and Prevention) and sequence-based typing. Samples taken from 9 of 18 patients were completely typed for TP0136, TP0548, 23S rDNA, arp, and tpr loci. The CDC typing revealed 11 distinct genotypes while the sequence-based typing identified 6 genotypes. When results from molecular typing of TP0136, TP0548, and 23S rDNA were analyzed in samples taken from the same patient, no discrepancies in the identified genotypes were found; however, there were discrepancies in 11 of 18 patients (61.1%) samples relative to the arp and tpr loci. In addition to the above described typing, 127 PCR-positive swabs and whole blood samples were tested for individual genotype frequencies. The repetition number for the arp gene was lower in whole blood (WB) samples compared to swab samples. Similarly, the most common tpr RFLP type "d" was found to have lower occurrence rates in WB samples while type "e" had an increased occurrence in these samples. Conclusions: Differences in the CDC subtypes identified in parallel samples indicated genetic instability of the arp and tpr loci and suggested limited applicability of the CDC typing system in epidemiological studies. Differences in treponemal genotypes detected in whole blood and swab samples suggested important differences between both compartments and/or differences in adherence of treponeme variants to human cells.
Links
GAP302/12/0574, research and development project |
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NT11159, research and development project |
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