Genome-wide transcriptional analysis upon entomopathogenic
nematode infection of Drosophila

a 2013

Genome-wide transcriptional analysis upon entomopathogenic nematode infection of Drosophila

KUČEROVÁ, Lucie, Badrul AREFIN, Pavel DOBEŠ, Robert MARKUS, Hynek STRNAD et. al.

Základní údaje

Originální název

Genome-wide transcriptional analysis upon entomopathogenic nematode infection of Drosophila

Autoři

KUČEROVÁ, Lucie (203 Česká republika), Badrul AREFIN (752 Švédsko), Pavel DOBEŠ (203 Česká republika, domácí), Robert MARKUS (348 Maďarsko), Hynek STRNAD (203 Česká republika), Zhi WANG (752 Švédsko), Pavel HYRŠL (203 Česká republika, garant, domácí), Michal ŽUROVEC (203 Česká republika) a Ulrich THEOPOLD (752 Švédsko)

Vydání

Integrated Insect Immunology: From Basic Biology To Environmental Applications, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30102 Immunology

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/13:00069822

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

Drosophila; entomopatogenní hlístice

Klíčová slova anglicky

Drosophila; entomopathogenic nematodes

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 1. 2016 23:23, Mgr. Pavel Dobeš, Ph.D.

Anotace

V originále

Entomopathogenic nematodes (EPN) Heterorhabditis bacteriophora are obligate and lethal insect parasites. These EPN are symbiotically associated with entomopathogenic bacteria Photorhabdus luminescens creating the highly pathogenic nematobacterial complex that is able to kill the host within 24 to 48 hours. H. bacteriophora with its bacterial symbionts are able to infect a broad spectrum of insect species. Symbiotic bacteria help to digest host tissues and provide nutrients for themselves and developing nematodes. Drosophila larvae are suitable insect hosts and part of the tripartite model system (Drosophila – EPN - bacteria). In this study we examined transcriptional changes in Drosophila upon nematode infection. We compare gene expression in non- infected and infected fly larvae. We focused on the early time point of nematode infection and therefore infected Drosophila larvae using H. bacteriophora harbouring GFP-labelled P. luminescens bacteria. Infected (GFP positive) larvae were collected 6 hours after infection. We found 642 genes significantly differentially regulated after nematobacterial infection caused by H. bacteriophora and P. luminescens. The majority of them are upregulated upon infection. The most strongly induced fraction comprise antimicrobial humoral response genes and defense response genes. However we observed also a lot of transcripts involved in development, morphogenesis and cell differentiation. Based on Gene Ontology annotation we identified several pathways, which could be involved in sealing and repairing the wound caused by invading nematodes. We compared our results with the available data for other infection types caused by bacteria (both nonpathogenic and pathogenic) and parasitic wasps. A core set of 17 genes was common for all types of infection and approximately 10-25% of genes were overlapping in each pairwise comparison.

Návaznosti

CZ.1.07/2.3.00/30.0009, interní kód MU
(Kód CEP: EE2.3.30.0009)

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci (Akronym: Postdoc I.)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci, 2.3 Lidské zdroje ve výzkumu a vývoji

Citovat

KUČEROVÁ, Lucie, Badrul AREFIN, Pavel DOBEŠ, Robert MARKUS, Hynek STRNAD, Zhi WANG, Pavel HYRŠL, Michal ŽUROVEC a Ulrich THEOPOLD. Genome-wide transcriptional analysis upon entomopathogenic nematode infection of Drosophila. In Integrated Insect Immunology: From Basic Biology To Environmental Applications. 2013.

@proceedings{1126624,
   author = {Kučerová, Lucie and Arefin, Badrul and Dobeš, Pavel and Markus, Robert and Strnad, Hynek and Wang, Zhi and Hyršl, Pavel and Žurovec, Michal and Theopold, Ulrich},
   booktitle = {Integrated Insect Immunology: From Basic Biology To Environmental Applications},
   keywords = {Drosophila; entomopathogenic nematodes},
   language = {eng},
   title = {Genome-wide transcriptional analysis upon entomopathogenic nematode infection of Drosophila},
   year = {2013}
}

TY  - CONF
ID  - 1126624
AU  - Kučerová, Lucie - Arefin, Badrul - Dobeš, Pavel - Markus, Robert - Strnad, Hynek - Wang, Zhi - Hyršl, Pavel - Žurovec, Michal - Theopold, Ulrich
PY  - 2013
TI  - Genome-wide transcriptional analysis upon entomopathogenic nematode infection of Drosophila
KW  - Drosophila
KW  - entomopathogenic nematodes
N2  - Entomopathogenic nematodes (EPN) Heterorhabditis bacteriophora are obligate and lethal insect parasites. These EPN are symbiotically associated with entomopathogenic bacteria Photorhabdus luminescens creating the highly pathogenic nematobacterial complex that is able to kill the host within 24 to 48 hours. H. bacteriophora with its bacterial symbionts are able to infect a broad spectrum of insect species. Symbiotic bacteria help to digest host tissues and provide nutrients for themselves and developing nematodes. Drosophila larvae are suitable insect hosts and part of the tripartite model system (Drosophila – EPN - bacteria). In this study we examined transcriptional changes in Drosophila upon nematode infection. We compare gene expression in non- infected and infected fly larvae. We focused on the early time point of nematode infection and therefore infected Drosophila larvae using H. bacteriophora harbouring GFP-labelled P. luminescens bacteria. Infected (GFP positive) larvae were collected 6 hours after infection. We found 642 genes significantly differentially regulated after nematobacterial infection caused by H. bacteriophora and P. luminescens. The majority of them are upregulated upon infection. The most strongly induced fraction comprise antimicrobial humoral response genes and defense response genes. However we observed also a lot of transcripts involved in development, morphogenesis and cell differentiation. Based on Gene Ontology annotation we identified several pathways, which could be involved in sealing and repairing the wound caused by invading nematodes. We compared our results with the available data for other infection types caused by bacteria (both nonpathogenic and pathogenic) and parasitic wasps. A core set of 17 genes was common for all types of infection and approximately 10-25% of genes were overlapping in each pairwise comparison.
ER  -

KUČEROVÁ, Lucie, Badrul AREFIN, Pavel DOBEŠ, Robert MARKUS, Hynek STRNAD, Zhi WANG, Pavel HYRŠL, Michal ŽUROVEC a Ulrich THEOPOLD. Genome-wide transcriptional analysis upon entomopathogenic nematode infection of Drosophila. In \textit{Integrated Insect Immunology: From Basic Biology To Environmental Applications}. 2013.

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