The role of ATP-binding transporters associated with multi-drug
resistance in stem cells

k 2013

The role of ATP-binding transporters associated with multi-drug resistance in stem cells

LÁNOVÁ, Martina, Josef VEČEŘA, Jan KUČERA, Jiřina MEDALOVÁ, Jiří PACHERNÍK et. al.

Základní údaje

Originální název

The role of ATP-binding transporters associated with multi-drug resistance in stem cells

Autoři

LÁNOVÁ, Martina (203 Česká republika, domácí), Josef VEČEŘA (203 Česká republika, domácí), Jan KUČERA (203 Česká republika, domácí), Jiřina MEDALOVÁ (203 Česká republika, domácí) a Jiří PACHERNÍK (203 Česká republika, garant, domácí)

Vydání

International Conference Analytical Cytometry VII Mikulov 2013, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30105 Physiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/13:00070040

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-905449-2-5

Klíčová slova anglicky

ABC-transporters neural stem cells

Změněno: 11. 3. 2015 10:52, RNDr. Josef Večeřa, Ph.D.

Anotace

V originále

ATP-binding transporters (ABC-t) play various roles in regulation organism function and homeostasis from prokaryota to mammals. ABC-t mediate transport of mainly lipophilic substances through cellular membranes. Some ABC-t are important in cell protection against endogenous and importantly also exogenous toxins. These transporters are called ABC-t associated with multi-drug resistance (ABC-t/MDR), according to their role in resistance of tumor cells to pharmacotherapy. ABC-t/MDR are also over-expressed in stem cells, where their protective role is expected, too. Particularly, ABCB1, ABCC1, and ABCG2 are common ABC-t/MDR expressed in stem cells. However, substrates of ABC-t/MDR are not only toxins, but also important signaling molecules as well leukotrienes and/or glutathione conjugates and porphyrins, which mediated balance in intracellular oxidation-reduction processing. Thus we hypothesize the role of ABC-t/MDR also in regulation of stem cells fate. To test this hypothesis we analyzed effect of modulation of ABC-t/MDR activity in embryonic and neural stem cells. We observed that ABCC1 and ABCG2 are the most expressed ABC-t/MDR in our tested stem cells. Importantly, inhibition of these ABC-t/MDR leads to decreasing of stemness and induction of differentiation in both embryonic and neural stem cells. Analysis of mechanism of observed effect and identification of studying ABC-t/MDR substrates, which may be responsible for this effect, are in progression.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

Citovat

LÁNOVÁ, Martina, Josef VEČEŘA, Jan KUČERA, Jiřina MEDALOVÁ a Jiří PACHERNÍK. The role of ATP-binding transporters associated with multi-drug resistance in stem cells. In International Conference Analytical Cytometry VII Mikulov 2013. 2013. ISBN 978-80-905449-2-5.

@proceedings{1128405,
   author = {Lánová, Martina and Večeřa, Josef and Kučera, Jan and Medalová, Jiřina and Pacherník, Jiří},
   booktitle = {International Conference Analytical Cytometry VII Mikulov 2013},
   keywords = {ABC-transporters neural stem cells},
   language = {eng},
   isbn = {978-80-905449-2-5},
   title = {The role of ATP-binding transporters associated with multi-drug resistance in stem cells},
   year = {2013}
}

TY  - CONF
ID  - 1128405
AU  - Lánová, Martina - Večeřa, Josef - Kučera, Jan - Medalová, Jiřina - Pacherník, Jiří
PY  - 2013
TI  - The role of ATP-binding transporters associated with multi-drug resistance in stem cells
SN  - 9788090544925
KW  - ABC-transporters neural stem cells
N2  - ATP-binding transporters (ABC-t) play various roles in regulation organism function and homeostasis from prokaryota to mammals. ABC-t mediate transport of mainly lipophilic substances through cellular membranes. Some ABC-t are important in cell protection against endogenous and importantly also exogenous toxins. These transporters are called ABC-t associated with multi-drug resistance (ABC-t/MDR), according to their role in resistance of tumor cells to pharmacotherapy. ABC-t/MDR are also over-expressed in stem cells, where their protective role is expected, too. Particularly, ABCB1, ABCC1, and ABCG2 are common ABC-t/MDR expressed in stem cells. However, substrates of ABC-t/MDR are not only toxins, but also important signaling molecules as well leukotrienes and/or glutathione conjugates and porphyrins, which mediated balance in intracellular oxidation-reduction processing. Thus we hypothesize the role of ABC-t/MDR also in regulation of stem cells fate. To test this hypothesis we analyzed effect of modulation of ABC-t/MDR activity in embryonic and neural stem cells. We observed that ABCC1 and ABCG2 are the most expressed ABC-t/MDR in our tested stem cells. Importantly, inhibition of these ABC-t/MDR leads to decreasing of stemness and induction of differentiation in both embryonic and neural stem cells. Analysis of mechanism of observed effect and identification of studying ABC-t/MDR substrates, which may be responsible for this effect, are in progression.
ER  -

LÁNOVÁ, Martina, Josef VEČEŘA, Jan KUČERA, Jiřina MEDALOVÁ a Jiří PACHERNÍK. The role of ATP-binding transporters associated with multi-drug resistance in stem cells. In \textit{International Conference Analytical Cytometry VII Mikulov 2013}. 2013. ISBN~978-80-905449-2-5.

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