EMT-related miRNAs as a potential markers for prediction and
early detection of metastasis in renal cell carcinoma

a 2013

EMT-related miRNAs as a potential markers for prediction and early detection of metastasis in renal cell carcinoma

MLČOCHOVÁ, Hana, Renata HÉŽOVÁ, Táňa MACHÁČKOVÁ, Martina LOJOVÁ, Alexandr POPRACH et. al.

Základní údaje

Originální název

EMT-related miRNAs as a potential markers for prediction and early detection of metastasis in renal cell carcinoma

Autoři

MLČOCHOVÁ, Hana (203 Česká republika, domácí), Renata HÉŽOVÁ (203 Česká republika, domácí), Táňa MACHÁČKOVÁ (203 Česká republika, domácí), Martina LOJOVÁ (203 Česká republika, domácí), Alexandr POPRACH (203 Česká republika), Radek LAKOMÝ (203 Česká republika), Pavel FABIAN (203 Česká republika), Marek SVOBODA (203 Česká republika), Rostislav VYZULA (203 Česká republika) a Ondřej SLABÝ (203 Česká republika, garant, domácí)

Vydání

TEMTIA 2013, The 6th International EMT Meeting, Alicante, Spain, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Španělsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14740/13:00065638

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

EMT; renal cell carcinoma; miRNAs; predictive biomarkers; metastases

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 11. 2013 11:50, Mgr. Petra Vychytilová, Ph.D.

Anotace

V originále

Renal cell carcinoma (RCC) belongs among the most common adult kidney neoplasm. Because of the lack of early warning signals, a high percentage of patients with metastatic RCC occur. Nowadays, epithelial-mesenchymal transition (EMT) as a crucial event of the tumor progression resulting in metastasis is intensively studied. EMT is a biological process in which polarized epithelial cells undergo multiple biochemical changes altering their phenotype from epithelial to mesenchymal. This phenotype change observed during the tumor metastasis development is also accompanied on a molecular level by miRNAs deregulation. In our study, we try to identify EMT-related miRNAs that can be used in clinical practice as a predictors of metastatic risk and early relapse of the RCC. Five EMT-related miRNAs were studied on 3 different groups of RCC patients. Using Kruskal-Wallis test, miR-192 (p=0,0003), miR-200b (p=0,0145) a miR-215 (p<0,0001) showed significantly different miRNAs expression level between studying groups of RCC patients. Our primary data suggest that EMT-related tissue miRNAs (miR-192, miR-200b, miR-215) could potentially serve as a markers of RCC for differenciation between patiens without/with progression and primary metastatic patiens. No signifiant differences between patiens with and without progression were observed, however miR-192 showed decreasing level of expression depending on higher metastatic potential of the tumor. Further studies are needed to confirm suitability of these miRNA markers for individualisation of RCC therapy and their potential use in the clinical practice.

Návaznosti

NT13547, projekt VaV

Název: Studium mikroRNA a genů asociovaných s procesem epiteliálně-mezenchymální tranzice jako potenciálních markerů pro predikci rizika a časný záchyt metastazování u pacientů s renálním karcinomem

Citovat

MLČOCHOVÁ, Hana, Renata HÉŽOVÁ, Táňa MACHÁČKOVÁ, Martina LOJOVÁ, Alexandr POPRACH, Radek LAKOMÝ, Pavel FABIAN, Marek SVOBODA, Rostislav VYZULA a Ondřej SLABÝ. EMT-related miRNAs as a potential markers for prediction and early detection of metastasis in renal cell carcinoma. In TEMTIA 2013, The 6th International EMT Meeting, Alicante, Spain. 2013.

@proceedings{1129965,
   author = {Mlčochová, Hana and Héžová, Renata and Macháčková, Táňa and Lojová, Martina and Poprach, Alexandr and Lakomý, Radek and Fabian, Pavel and Svoboda, Marek and Vyzula, Rostislav and Slabý, Ondřej},
   booktitle = {TEMTIA 2013, The 6th International EMT Meeting, Alicante, Spain},
   keywords = {EMT; renal cell carcinoma; miRNAs; predictive biomarkers; metastases},
   language = {eng},
   title = {EMT-related miRNAs as a potential markers for prediction and early detection of metastasis in renal cell carcinoma},
   year = {2013}
}

TY  - CONF
ID  - 1129965
AU  - Mlčochová, Hana - Héžová, Renata - Macháčková, Táňa - Lojová, Martina - Poprach, Alexandr - Lakomý, Radek - Fabian, Pavel - Svoboda, Marek - Vyzula, Rostislav - Slabý, Ondřej
PY  - 2013
TI  - EMT-related miRNAs as a potential markers for prediction and early detection of metastasis in renal cell carcinoma
KW  - EMT
KW  - renal cell carcinoma
KW  - miRNAs
KW  - predictive biomarkers
KW  - metastases
N2  - Renal cell carcinoma (RCC) belongs among the most common adult kidney neoplasm. Because of the lack of early warning signals, a high percentage of patients with metastatic RCC occur. Nowadays, epithelial-mesenchymal transition (EMT) as a crucial event of the tumor progression resulting in metastasis is intensively studied. EMT is a biological process in which polarized epithelial cells undergo multiple biochemical changes altering their phenotype from epithelial to mesenchymal. This phenotype change observed during the tumor metastasis development is also accompanied on a molecular level by miRNAs deregulation. In our study, we try to identify EMT-related miRNAs that can be used in clinical practice as a predictors of metastatic risk and early relapse of the RCC. Five EMT-related miRNAs were studied on 3 different groups of RCC patients. Using Kruskal-Wallis test, miR-192 (p=0,0003), miR-200b (p=0,0145) a miR-215 (p<0,0001) showed significantly different miRNAs expression level between studying groups of RCC patients. Our primary data suggest that EMT-related tissue miRNAs (miR-192, miR-200b, miR-215) could potentially serve as a markers of RCC for differenciation between patiens without/with progression and primary metastatic patiens. No signifiant differences between patiens with and without progression were observed, however miR-192 showed decreasing level of expression depending on higher metastatic potential of the tumor. Further studies are needed to confirm suitability of these miRNA markers for individualisation of RCC therapy and their potential use in the clinical practice.
ER  -

MLČOCHOVÁ, Hana, Renata HÉŽOVÁ, Táňa MACHÁČKOVÁ, Martina LOJOVÁ, Alexandr POPRACH, Radek LAKOMÝ, Pavel FABIAN, Marek SVOBODA, Rostislav VYZULA a Ondřej SLABÝ. EMT-related miRNAs as a potential markers for prediction and early detection of metastasis in renal cell carcinoma. In \textit{TEMTIA 2013, The 6th International EMT Meeting, Alicante, Spain}. 2013.

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