Alkaloid chelirubine and DNA: Blue and red luminescence

RÁJECKÝ, Michal, Iva SLANINOVÁ, Petra MOKRIŠOVÁ, Jana URBANOVÁ, Martin PALKOVSKÝ, Eva TÁBORSKÁ and Petr TÁBORSKÝ. Alkaloid chelirubine and DNA: Blue and red luminescence. Talanta. Amsterdam: Elsevier Science, 2013, vol. 105, únor, p. 317-319. ISSN 0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2012.10.045.

Basic information

• Original name: Alkaloid chelirubine and DNA: Blue and red luminescence
• Name in Czech: Alkaloid chelirubin a DNA: Modrá a červená luminiscence
• Authors: RÁJECKÝ, Michal (203 Czech Republic, belonging to the institution), Iva SLANINOVÁ (203 Czech Republic, belonging to the institution), Petra MOKRIŠOVÁ (703 Slovakia, belonging to the institution), Jana URBANOVÁ (203 Czech Republic, belonging to the institution), Martin PALKOVSKÝ (203 Czech Republic), Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution) and Petr TÁBORSKÝ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Talanta, Amsterdam, Elsevier Science, 2013, 0039-9140.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.511
• RIV identification code: RIV/00216224:14310/13:00070283
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1016/j.talanta.2012.10.045
• UT WoS: 000319088500046
• Keywords (in Czech): Alkaloid; Benzo[c]fenanthridin; Chelirubin; DNA; Interkalace; Fluorescence
• Keywords in English: Alkaloid; Benzo[c]phenanthridine; Chelirubine; DNA; Intercalation; Fluorescence
• Tags: AKR, podil, rivok
• Tags: International impact, Reviewed

Abstract

Extracts from Himalayan herb Dicranostigma lactucoides containing alkaloid chelirubine have been used for centuries in Chinese herbal medicine. We have found a new employment for the alkaloid: it can be used as a DNA fluorescent probe showing blue (free form) and red (intercalated to DNA) luminescence emission after irradiation by violet light. Besides quantification of DNA (LOD = 6 ng.ml-1) it can also be used as a supravital cell probe because chelirubine molecules can effectively enter to the living cell through the cell membrane.

Links

• LH12176, research and development project: Name: Benzofenanthridinové alkaloidy - studium účinků na celulární a molekulární úrovni (Acronym: KBF)

Investor: Ministry of Education, Youth and Sports of the CR

• MUNI/A/0798/2012, interní kód MU: Name: Molekulárně- a buněčně-biologický základ biomedicíny (Acronym: MBBZB)

Investor: Masaryk University, Category A

• MUNI/A/0818/2012, interní kód MU: Name: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: BIOMET 2)

Investor: Masaryk University, Category A