Alkaloid chelirubine and DNA: Blue and red luminescence

J 2013

Alkaloid chelirubine and DNA: Blue and red luminescence

RÁJECKÝ, Michal, Iva SLANINOVÁ, Petra MOKRIŠOVÁ, Jana URBANOVÁ, Martin PALKOVSKÝ et. al.

Basic information

Original name

Alkaloid chelirubine and DNA: Blue and red luminescence

Name in Czech

Alkaloid chelirubin a DNA: Modrá a červená luminiscence

Authors

RÁJECKÝ, Michal (203 Czech Republic, belonging to the institution), Iva SLANINOVÁ (203 Czech Republic, belonging to the institution), Petra MOKRIŠOVÁ (703 Slovakia, belonging to the institution), Jana URBANOVÁ (203 Czech Republic, belonging to the institution), Martin PALKOVSKÝ (203 Czech Republic), Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution) and Petr TÁBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Talanta, Amsterdam, Elsevier Science, 2013, 0039-9140

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.511

RIV identification code

RIV/00216224:14310/13:00070283

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.talanta.2012.10.045

UT WoS

000319088500046

Keywords (in Czech)

Alkaloid; Benzo[c]fenanthridin; Chelirubin; DNA; Interkalace; Fluorescence

Keywords in English

Alkaloid; Benzo[c]phenanthridine; Chelirubine; DNA; Intercalation; Fluorescence

Abstract

V originále

Extracts from Himalayan herb Dicranostigma lactucoides containing alkaloid chelirubine have been used for centuries in Chinese herbal medicine. We have found a new employment for the alkaloid: it can be used as a DNA fluorescent probe showing blue (free form) and red (intercalated to DNA) luminescence emission after irradiation by violet light. Besides quantification of DNA (LOD = 6 ng.ml-1) it can also be used as a supravital cell probe because chelirubine molecules can effectively enter to the living cell through the cell membrane.

Links

LH12176, research and development project

Name: Benzofenanthridinové alkaloidy - studium účinků na celulární a molekulární úrovni (Acronym: KBF)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/0798/2012, interní kód MU

Name: Molekulárně- a buněčně-biologický základ biomedicíny (Acronym: MBBZB)

Investor: Masaryk University, Category A

MUNI/A/0818/2012, interní kód MU

Name: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: BIOMET 2)

Investor: Masaryk University, Category A

Citovat

RÁJECKÝ, Michal, Iva SLANINOVÁ, Petra MOKRIŠOVÁ, Jana URBANOVÁ, Martin PALKOVSKÝ, Eva TÁBORSKÁ and Petr TÁBORSKÝ. Alkaloid chelirubine and DNA: Blue and red luminescence. Talanta. Amsterdam: Elsevier Science, 2013, vol. 105, únor, p. 317-319. ISSN 0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2012.10.045.

@article{1131043,
   author = {Rájecký, Michal and Slaninová, Iva and Mokrišová, Petra and Urbanová, Jana and Palkovský, Martin and Táborská, Eva and Táborský, Petr},
   article_location = {Amsterdam},
   article_number = {únor},
   doi = {http://dx.doi.org/10.1016/j.talanta.2012.10.045},
   keywords = {Alkaloid; Benzo[c]phenanthridine; Chelirubine; DNA; Intercalation; Fluorescence},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Alkaloid chelirubine and DNA: Blue and red luminescence},
   url = {http://www.sciencedirect.com/science/article/pii/S0039914012008764},
   volume = {105},
   year = {2013}
}

TY  - JOUR
ID  - 1131043
AU  - Rájecký, Michal - Slaninová, Iva - Mokrišová, Petra - Urbanová, Jana - Palkovský, Martin - Táborská, Eva - Táborský, Petr
PY  - 2013
TI  - Alkaloid chelirubine and DNA: Blue and red luminescence
JF  - Talanta
VL  - 105
IS  - únor
SP  - 317-319
EP  - 317-319
PB  - Elsevier Science
SN  - 00399140
KW  - Alkaloid
KW  - Benzo[c]phenanthridine
KW  - Chelirubine
KW  - DNA
KW  - Intercalation
KW  - Fluorescence
UR  - http://www.sciencedirect.com/science/article/pii/S0039914012008764
L2  - http://www.sciencedirect.com/science/article/pii/S0039914012008764
N2  - Extracts from Himalayan herb Dicranostigma lactucoides containing alkaloid chelirubine have been used for centuries in Chinese herbal medicine. We have found a new employment for the alkaloid: it can be used as a DNA fluorescent probe showing blue (free form) and red (intercalated to DNA) luminescence emission after irradiation by violet light. Besides quantification of DNA (LOD = 6 ng.ml-1) it can also be used as a supravital cell probe because chelirubine molecules can effectively enter to the living cell through the cell membrane.
ER  -

RÁJECKÝ, Michal, Iva SLANINOVÁ, Petra MOKRIŠOVÁ, Jana URBANOVÁ, Martin PALKOVSKÝ, Eva TÁBORSKÁ and Petr TÁBORSKÝ. Alkaloid chelirubine and DNA: Blue and red luminescence. \textit{Talanta}. Amsterdam: Elsevier Science, 2013, vol.~105, únor, p.~317-319. ISSN~0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2012.10.045.

Detailed Information on Publication Record