Detailed Information on Publication Record
2014
Incidence of cytogenetic aberrations in two B lineage subpopulations in multiple myeloma patients analyzed by combination of whole-genome profiling and FISH
SMETANA, Jan, Elena Vladimirovna KRYUKOVA, Fedor KRYUKOV, Pavel NĚMEC, Henrieta GREŠLIKOVÁ et. al.Basic information
Original name
Incidence of cytogenetic aberrations in two B lineage subpopulations in multiple myeloma patients analyzed by combination of whole-genome profiling and FISH
Authors
SMETANA, Jan (203 Czech Republic, belonging to the institution), Elena Vladimirovna KRYUKOVA (643 Russian Federation, belonging to the institution), Fedor KRYUKOV (643 Russian Federation, belonging to the institution), Pavel NĚMEC (203 Czech Republic, belonging to the institution), Henrieta GREŠLIKOVÁ (703 Slovakia, belonging to the institution), Renata KUPSKÁ (203 Czech Republic, belonging to the institution), Aneta MIKULÁŠOVÁ (203 Czech Republic, belonging to the institution), Ivana IHNATOVÁ (703 Slovakia, belonging to the institution), Roman HÁJEK (203 Czech Republic, guarantor, belonging to the institution) and Petr KUGLÍK (203 Czech Republic, belonging to the institution)
Edition
Neoplasma, Bratislava, Slovenská akademie vied, 2014, 0028-2685
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30200 3.2 Clinical medicine
Country of publisher
Slovakia
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.865
RIV identification code
RIV/00216224:14110/14:00073433
Organization unit
Faculty of Medicine
UT WoS
000329769500007
Keywords in English
multiple myeloma; CD19+; CD138+; cytogenetics; array-CGH; FISH
Tags
Tags
International impact, Reviewed
Změněno: 22/4/2014 12:39, Ing. Mgr. Věra Pospíšilíková
Abstract
V originále
Multiple myeloma (MM) is an incurable malignant disease of the terminal developmental stage of B-lymphocytes. While genetic heterogeneity of MM is widely described, little is known about its genetic basis as well as primary damage during plasma cells (PC) development. In this study, we focused on genome-wide screening of DNA copy number changes using oligonucleotide-based array-CGH together with I-FISH of the IgH locus rearrangements in pair samples of bone marrow B-cells (CD19+) and CD138+ PC from newly diagnosed MM patients. The IgH disruption was found in 8.9% (4/45) of CD19+ samples and in 57.8% (26/45) of CD138+ samples. The genomic profiling using array-CGH identified copy number alterations (CNAs) in 10% (2/20) of CD19+ samples in regions known to be important for MM pathogenesis. In contrast, we found CNAs in 100% (16/16) of CD138+ samples. Most common chromosomal abnormalities were trisomies of odd-numbered chromosomes (3, 5, 7, 9, 11, 15, 19 and 21), gain 1q, gain Xq and monosomy of chromosome 13. We did not find any correlation between incidence of CNAs in CD19+ and CD138+ cells. In conclusion, effective utilization of FISH and array-CGH can identify genetic lesions in premalignant stages leading to better understanding and characterization of MM.
Links
| GAP304/10/1395, research and development project |
| ||
| MSM0021622434, plan (intention) |
| ||
| NS10207, research and development project |
| ||
| NT11154, research and development project |
| ||
| NT13190, research and development project |
| ||
| NT13492, research and development project |
|