Piezoelectric biosensor coupled to cyclone air sampler for
detection of microorganisms

J 2013

Piezoelectric biosensor coupled to cyclone air sampler for detection of microorganisms

FARKA, Zdeněk, David KOVÁŘ a Petr SKLÁDAL

Základní údaje

Originální název

Piezoelectric biosensor coupled to cyclone air sampler for detection of microorganisms

Autoři

FARKA, Zdeněk (203 Česká republika, domácí), David KOVÁŘ (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

Chemické listy, Praha, Česká společnost chemická, 2013, 0009-2770

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

zacina na 14. strance v souboru

Impakt faktor

Impact factor: 0.196

Kód RIV

RIV/00216224:14310/13:00070523

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000328730800008

Klíčová slova česky

bioaerosol; biosensor; imunosensor; QCM

Klíčová slova anglicky

Bioaerosol; Biosensor; Immunosensor; QCM

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 3. 2014 11:27, prof. RNDr. Petr Skládal, CSc.

Anotace

ORIG CZ

V originále

Even though microorganisms are common part of our lives, some of them are dangerous and must be monitored. The traditional microbiological methods are reliable but slow. In some cases, namely in military, key requirement is fast detection which can be provided by biosensors. In this work, the piezoelectric biosensors providing fast, specific and cheap way of detection are described. Three strains of E. coli (BL21, DH5 alpha and K-12) were chosen as safe model microbes and the detection using the developed piezoelectric biosensor was carried out (LOD 10^6 CFU mL-1, total analysis time 20 min). Bioaerosol chamber was constructed to allow safe dissemination of microbes in air and experiments with bioaerosol. The generated bioaerosol was sampled using the cyclone air sampler and analyzed with the on-line coupled piezoelectric biosensor. The levels of 10^6 CFU L-1 of E. coli in air were successfully detected in less than 26 min which demonstrates possible application of this system for rapid screening of microorganisms in the air.

Česky

Piezoelectrický imunosensor pro detekci kmenů Escherichia coli (BL21, DH5 alpha, K-12) ve formě bioaerosolu.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

FARKA, Zdeněk, David KOVÁŘ a Petr SKLÁDAL. Piezoelectric biosensor coupled to cyclone air sampler for detection of microorganisms. Chemické listy. Praha: Česká společnost chemická, 2013, roč. 107, s3, s. s302-s304, 3 s. ISSN 0009-2770.

@article{1136376,
   author = {Farka, Zdeněk and Kovář, David and Skládal, Petr},
   article_location = {Praha},
   article_number = {s3},
   keywords = {Bioaerosol; Biosensor; Immunosensor; QCM},
   language = {eng},
   issn = {0009-2770},
   journal = {Chemické listy},
   title = {Piezoelectric biosensor coupled to cyclone air sampler for detection of microorganisms},
   url = {http://www.chemicke-listy.cz/docs/full/2013_s3_s289-s311.pdf},
   volume = {107},
   year = {2013}
}

TY  - JOUR
ID  - 1136376
AU  - Farka, Zdeněk - Kovář, David - Skládal, Petr
PY  - 2013
TI  - Piezoelectric biosensor coupled to cyclone air sampler for detection of microorganisms
JF  - Chemické listy
VL  - 107
IS  - s3
SP  - s302-s304
EP  - s302-s304
PB  - Česká společnost chemická
SN  - 00092770
KW  - Bioaerosol
KW  - Biosensor
KW  - Immunosensor
KW  - QCM
UR  - http://www.chemicke-listy.cz/docs/full/2013_s3_s289-s311.pdf
L2  - http://www.chemicke-listy.cz/docs/full/2013_s3_s289-s311.pdf
N2  - Even though microorganisms are common part of our lives, some of them are dangerous and must be monitored. The traditional microbiological methods are reliable but slow. In some cases, namely in military, key requirement is fast detection which can be provided by biosensors. In this work, the piezoelectric biosensors providing fast, specific and cheap way of detection are described. Three strains of E. coli (BL21, DH5 alpha and K-12) were chosen as safe model microbes and the detection using the developed piezoelectric biosensor was carried out (LOD 10^6 CFU mL-1, total analysis time 20 min). Bioaerosol chamber was constructed to allow safe dissemination of microbes in air and experiments with bioaerosol. The generated bioaerosol was sampled using the cyclone air sampler and analyzed with the on-line coupled piezoelectric biosensor. The levels of 10^6 CFU L-1 of E. coli in air were successfully detected in less than 26 min which demonstrates possible application of this system for rapid screening of microorganisms in the air.
ER  -

FARKA, Zdeněk, David KOVÁŘ a Petr SKLÁDAL. Piezoelectric biosensor coupled to cyclone air sampler for detection of microorganisms. \textit{Chemické listy}. Praha: Česká společnost chemická, 2013, roč.~107, s3, s.~s302-s304, 3 s. ISSN~0009-2770.

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