Microfluidic chip coupled with modified paramagnetic particles
for sarcosine isolation in urine

J 2013

Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine

ZITKA, Ondrej, Natalia CERNEI, Zbynek HEGER, Miroslav MATOUSEK, Pavel KOPEL et. al.

Základní údaje

Originální název

Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine

Autoři

ZITKA, Ondrej (203 Česká republika), Natalia CERNEI (203 Česká republika), Zbynek HEGER (203 Česká republika), Miroslav MATOUSEK (203 Česká republika), Pavel KOPEL (203 Česká republika), Jindrich KYNICKY (203 Česká republika), Michal MASAŘÍK (203 Česká republika, garant, domácí), Rene KIZEK (203 Česká republika) a Vojtech ADAM (203 Česká republika)

Vydání

Electrophoresis, Hoboken, Wiley-Blackwell, 2013, 0173-0835

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.161

Kód RIV

RIV/00216224:14110/13:00070631

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/elps.201300114

UT WoS

000327590900006

Klíčová slova anglicky

Ion exchange chromatography; Magnetic nanoparticles; Microfluidic separation; Prostate cancer; Surface modification

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 4. 2014 12:34, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Carcinoma of prostate (CaP) is the second most frequent malignant tumor occurring in men in Europe. Currently there is discussion on a wide range of potential CaP markers. One of them-nonprotein amino acid sarcosine, also known as N-methylglycine was chosen as a challenge for the development of microfluidic system with isolation by modified paramagnetic microparticles. Therefore, the aim of this study was to design a low-cost, simple, and rapid microfluidic system based on sarcosine isolation with modified paramagnetic microparticles and subsequent analysis on the ion exchange LC. We modified Dowex microparticles with Fe2O3 nanoparticles. Our paramagnetic microparticles were able to establish the binding with sarcosine. Moreover, we designed microfluidic device for sarcosine determination. Analysis of samples was carried out with LOD of 1 uM of a sarcosine that is sufficient because it is similar to concentrations of a sarcosine observed in the CaP patients.

Citovat

ZITKA, Ondrej, Natalia CERNEI, Zbynek HEGER, Miroslav MATOUSEK, Pavel KOPEL, Jindrich KYNICKY, Michal MASAŘÍK, Rene KIZEK a Vojtech ADAM. Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine. Electrophoresis. Hoboken: Wiley-Blackwell, 2013, roč. 34, č. 18, s. 2639-2647. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201300114.

@article{1137575,
   author = {Zitka, Ondrej and Cernei, Natalia and Heger, Zbynek and Matousek, Miroslav and Kopel, Pavel and Kynicky, Jindrich and Masařík, Michal and Kizek, Rene and Adam, Vojtech},
   article_location = {Hoboken},
   article_number = {18},
   doi = {http://dx.doi.org/10.1002/elps.201300114},
   keywords = {Ion exchange chromatography; Magnetic nanoparticles; Microfluidic separation; Prostate cancer; Surface modification},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine},
   volume = {34},
   year = {2013}
}

TY  - JOUR
ID  - 1137575
AU  - Zitka, Ondrej - Cernei, Natalia - Heger, Zbynek - Matousek, Miroslav - Kopel, Pavel - Kynicky, Jindrich - Masařík, Michal - Kizek, Rene - Adam, Vojtech
PY  - 2013
TI  - Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine
JF  - Electrophoresis
VL  - 34
IS  - 18
SP  - 2639-2647
EP  - 2639-2647
PB  - Wiley-Blackwell
SN  - 01730835
KW  - Ion exchange chromatography
KW  - Magnetic nanoparticles
KW  - Microfluidic separation
KW  - Prostate cancer
KW  - Surface modification
N2  - Carcinoma of prostate (CaP) is the second most frequent malignant tumor occurring in men in Europe. Currently there is discussion on a wide range of potential CaP markers. One of them-nonprotein amino acid sarcosine, also known as N-methylglycine was chosen as a challenge for the development of microfluidic system with isolation by modified paramagnetic microparticles. Therefore, the aim of this study was to design a low-cost, simple, and rapid microfluidic system based on sarcosine isolation with modified paramagnetic microparticles and subsequent analysis on the ion exchange LC. We modified Dowex microparticles with Fe2O3 nanoparticles. Our paramagnetic microparticles were able to establish the binding with sarcosine. Moreover, we designed microfluidic device for sarcosine determination. Analysis of samples was carried out with LOD of 1 uM of a sarcosine that is sufficient because it is similar to concentrations of a sarcosine observed in the CaP patients.
ER  -

ZITKA, Ondrej, Natalia CERNEI, Zbynek HEGER, Miroslav MATOUSEK, Pavel KOPEL, Jindrich KYNICKY, Michal MASAŘÍK, Rene KIZEK a Vojtech ADAM. Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine. \textit{Electrophoresis}. Hoboken: Wiley-Blackwell, 2013, roč.~34, č.~18, s.~2639-2647. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201300114.

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