Inflammatory profiling of the satellite glial cells in the
dorsal root ganglia of rat experimental neuropathic pain models

a 2013

Inflammatory profiling of the satellite glial cells in the dorsal root ganglia of rat experimental neuropathic pain models

DUBOVÝ, Petr; Václav BRÁZDA; Ivana HRADILOVÁ SVÍŽENSKÁ; Ilona KLUSÁKOVÁ; Lucie KUBÍČKOVÁ et. al.

Basic information

Original name

Inflammatory profiling of the satellite glial cells in the dorsal root ganglia of rat experimental neuropathic pain models

Name in Czech

Zánětová charakteristika satelitních gliových buněk spinálních ganglií v experimentálních modelech neuropatické bolesti

Authors

DUBOVÝ, Petr; Václav BRÁZDA; Ivana HRADILOVÁ SVÍŽENSKÁ; Ilona KLUSÁKOVÁ and Lucie KUBÍČKOVÁ

Edition

The XI European Meeting on Glial Cell Function in Health and Disease, Berlin, 2013

Other information

Language

English

Type of outcome

Conference abstract

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Germany

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 5.466

Organization unit

Central European Institute of Technology

ISSN

UT WoS

000320408400539

Keywords (in Czech)

satelitní gliové buňky, cytokiny, neuropatická bolest

Keywords in English

satellite glial cells, cytokines, neuropathic pain

Changed: 23/12/2013 15:10, prof. RNDr. Petr Dubový, CSc.

Abstract

ORIG CZ

V originále

The satellite glial cells (SGC) envelope the primary sensory neurons of the dorsal root ganglia (DRG) and react intensely to various types of nerve injury that induce neuropathic pain. Unilateral chronic constriction injury (CCI, n=64) and spare nerve injury (SNI, n=32) of the rat sciatic nerve well as i.p. application of paclitaxel (PAC, n=12) were used as experimental models of neuropathic pain (NPP). NPP induction was tested by withdrawal threshold of mechanoallodynia and thermal hyperalgesia. Expression of TNFa, IL-6 and their receptor and signaling (TNFR1, TNFR2, IL-6R, gp130, STAT3) proteins and mRNA were investigated bilaterally by immunohistochemistry and in situ hybridization in both lumbar (L4-5) and cervical (C7-8) DRG 1, 3, 7 and 14 days from treatment. The SGC were identified by colocalization with GFAP or GS. Our results demonstrate significant inflammatory activation of the neurons and their SGC not only in DRG associated but also non-associated with injured nerve. A distinct expression of cytokines and their receptors was identified in SGC surrounding large-sized DRG neurons. Significant inflammatory reactions of SGC were found in all DRG of PAC-treated rats. Moreover, inflammatory activation SGC was also observed in DRG of sham-operated rats indicating other kinds of trigger than traumatic nerve injury. The nerve injury and PAC-treatment also triggered SOCS3 expression in SGC to control STAT3 activation. Inflammatory activation of SGC significantly contributes to ectopic activation of the DRG neurons not only associated with injured nerve, and is involved in NPP induction.

In Czech

Výsledky prokázaly zánětovou aktivaci satelitních gliových buněk v modelech neuropatické bolesti.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

286154, interní kód MU

Name: SYLICA - Synergies of Life and Material Sciences to Create a New Future (Acronym: SYLICA)

Investor: European Union, Capacities

Cite

DUBOVÝ, Petr; Václav BRÁZDA; Ivana HRADILOVÁ SVÍŽENSKÁ; Ilona KLUSÁKOVÁ and Lucie KUBÍČKOVÁ. Inflammatory profiling of the satellite glial cells in the dorsal root ganglia of rat experimental neuropathic pain models. In The XI European Meeting on Glial Cell Function in Health and Disease, Berlin. 2013. ISSN 0894-1491.

@proceedings{1138009,
   author = {Dubový, Petr and Brázda, Václav and Hradilová Svíženská, Ivana and Klusáková, Ilona and Kubíčková, Lucie},
   booktitle = {The XI European Meeting on Glial Cell Function in Health and Disease, Berlin},
   keywords = {satellite glial cells, cytokines, neuropathic pain},
   language = {eng},
   title = {Inflammatory profiling of the satellite glial cells in the dorsal root ganglia of rat experimental neuropathic pain models},
   year = {2013}
}

TY  - CONF
ID  - 1138009
AU  - Dubový, Petr - Brázda, Václav - Hradilová Svíženská, Ivana - Klusáková, Ilona - Kubíčková, Lucie
PY  - 2013
TI  - Inflammatory profiling of the satellite glial cells in the dorsal root ganglia of rat experimental neuropathic pain models
KW  - satellite glial cells, cytokines, neuropathic pain
N2  - The satellite glial cells (SGC) envelope the primary sensory neurons of the dorsal root ganglia (DRG) and react intensely to various types of nerve injury that induce neuropathic pain. Unilateral chronic constriction injury (CCI, n=64) and spare nerve injury (SNI, n=32) of the rat sciatic nerve well as i.p. application of paclitaxel (PAC, n=12) were used as experimental models of neuropathic pain (NPP). NPP induction was tested by withdrawal threshold of mechanoallodynia and thermal hyperalgesia. Expression of TNFa, IL-6 and their receptor and signaling (TNFR1, TNFR2, IL-6R, gp130, STAT3) proteins and mRNA were investigated bilaterally by immunohistochemistry and in situ hybridization in both lumbar (L4-5) and cervical (C7-8) DRG 1, 3, 7 and 14 days from treatment. The SGC were identified by colocalization with GFAP or GS. Our results demonstrate significant inflammatory activation of the neurons and their SGC not only in DRG associated but also non-associated with injured nerve. A distinct expression of cytokines and their receptors was identified in SGC surrounding large-sized DRG neurons. Significant inflammatory reactions of SGC were found in all DRG of PAC-treated rats. Moreover, inflammatory activation SGC was also observed in DRG of sham-operated rats indicating other kinds of trigger than traumatic nerve injury. The nerve injury and PAC-treatment also triggered SOCS3 expression in SGC to control STAT3 activation. Inflammatory activation of SGC significantly contributes to ectopic activation of the DRG neurons not only associated with injured nerve, and is involved in NPP induction.
ER  -

DUBOVÝ, Petr; Václav BRÁZDA; Ivana HRADILOVÁ SVÍŽENSKÁ; Ilona KLUSÁKOVÁ and Lucie KUBÍČKOVÁ. Inflammatory profiling of the satellite glial cells in the dorsal root ganglia of rat experimental neuropathic pain models. In \textit{The XI European Meeting on Glial Cell Function in Health and Disease, Berlin}. 2013. ISSN~0894-1491.

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