Inflammatory activation of satellite glial cells in the dorsal
root ganglia after nerve and skin injury

p 2013

Inflammatory activation of satellite glial cells in the dorsal root ganglia after nerve and skin injury

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ a Václav BRÁZDA

Základní údaje

Originální název

Inflammatory activation of satellite glial cells in the dorsal root ganglia after nerve and skin injury

Název česky

Zánětová aktivace satelitních gliových buněk ve spinálních gangliích po poškození nervu a kůže

Autoři

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ a Václav BRÁZDA

Vydání

SATELLITE SYMPOSIUM-NEUROINFLAMMATION to the regional FENS meeting, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Vyžádané přednášky

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

satelitní gliové buňky, poškození kůže a nervu

Klíčová slova anglicky

satellite glial cells, skin and nerve injury

Změněno: 23. 12. 2013 13:49, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

The satellite glial cells (SGC) envelope the primary sensory neurons of the dorsal root ganglia (DRG) and react intensely to various types of nerve injury that induce neuropathic pain. Inflammatory activation of SGC after skin incision is still not well understood. Unilateral chronic constriction injury (CCI, n=64) of the sciatic nerve and skin incision in thigh (SIT, n=32) performed under aseptic conditions were used as experimental model of neuropathic (NPP) and surgical pain, respectively. Withdrawal threshold of mechanical and thermal hyperalgesia were tested bilaterally in hairless skin of fore and hind paws. Expression of TNFa, IL-6, their receptors and signaling proteins and mRNAs were investigated bilaterally by immunohistochemistry and in situ hybridization in both lumbar (L4-5) and cervical (C7-8) DRG 1, 3, 7 and 14 days from surgery. The SGC were identified by colocalization with GFAP or GS. Ipsilateral hind paws of all CCI-operated rats displayed mechanical and thermal hyperalgesia from 1 to 14 days, but temporal changes were measured in contralateral hind paws and forepaws of both sides. Hyperalgesia was measured in SIT-operated animals only during early periods of survival (1-3 days). Inflammatory activation of DRG neurons and their SGC detected by upregulation of cytokines and their receptors was found in DRG after nerve injury, while only SGC were activated after skin incision. These results suggest different mechanisms inducing inflammatory reactions in DRG and their regulation after traumatic nerve injury and skin incision.

Česky

Satelitní gliové buňky spinálních ganglií byly aktivovány a produkovaly cytokiny po poškození kůže a nervu.

Návaznosti

286154, interní kód MU

Název: SYLICA - Synergies of Life and Material Sciences to Create a New Future (Akronym: SYLICA)

Investor: Evropská unie, SYLICA - Synergies of Life and Material Sciences to Create a New Future, Kapacity

Citovat

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ a Václav BRÁZDA. Inflammatory activation of satellite glial cells in the dorsal root ganglia after nerve and skin injury. In SATELLITE SYMPOSIUM-NEUROINFLAMMATION to the regional FENS meeting. 2013.

@misc{1138011,
   author = {Dubový, Petr and Klusáková, Ilona and Hradilová Svíženská, Ivana and Brázda, Václav},
   booktitle = {SATELLITE SYMPOSIUM-NEUROINFLAMMATION to the regional FENS meeting},
   keywords = {satellite glial cells, skin and nerve injury},
   language = {eng},
   title = {Inflammatory activation of satellite glial cells in the dorsal root ganglia after nerve and skin injury},
   year = {2013}
}

TY  - SLIDE
ID  - 1138011
AU  - Dubový, Petr - Klusáková, Ilona - Hradilová Svíženská, Ivana - Brázda, Václav
PY  - 2013
TI  - Inflammatory activation of satellite glial cells in the dorsal root ganglia after nerve and skin injury
KW  - satellite glial cells, skin and nerve injury
N2  - The satellite glial cells (SGC) envelope the primary sensory neurons of the dorsal root ganglia (DRG) and react intensely to various types of nerve injury that induce neuropathic pain. Inflammatory activation of SGC after skin incision is still not well understood. Unilateral chronic constriction injury (CCI, n=64) of the sciatic nerve and skin incision in thigh (SIT, n=32) performed under aseptic conditions were used as experimental model of neuropathic (NPP) and surgical pain, respectively. Withdrawal threshold of mechanical and thermal hyperalgesia were tested bilaterally in hairless skin of fore and hind paws. Expression of TNFa, IL-6, their receptors and signaling proteins and mRNAs were investigated bilaterally by immunohistochemistry and in situ hybridization in both lumbar (L4-5) and cervical (C7-8) DRG 1, 3, 7 and 14 days from surgery. The SGC were identified by colocalization with GFAP or GS. Ipsilateral hind paws of all CCI-operated rats displayed mechanical and thermal hyperalgesia from 1 to 14 days, but temporal changes were measured in contralateral hind paws and forepaws of both sides. Hyperalgesia was measured in SIT-operated animals only during early periods of survival (1-3 days). Inflammatory activation of DRG neurons and their SGC detected by upregulation of cytokines and their receptors was found in DRG after nerve injury, while only SGC were activated after skin incision. These results suggest different mechanisms inducing inflammatory reactions in DRG and their regulation after traumatic nerve injury and skin incision.
ER  -

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ a Václav BRÁZDA. Inflammatory activation of satellite glial cells in the dorsal root ganglia after nerve and skin injury. In \textit{SATELLITE SYMPOSIUM-NEUROINFLAMMATION to the regional FENS meeting}. 2013.

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