UPREGULATION OF IL-6 AND ITS SIGNALING MOLECULES IN THE DORSAL
ROOT GANGLIA OF LUMBAR AND CERVICAL SEGMENTS AND THEIR
INVOLVEMENT IN NEUROPATHIC PAIN INDUCTION

k 2013

UPREGULATION OF IL-6 AND ITS SIGNALING MOLECULES IN THE DORSAL ROOT GANGLIA OF LUMBAR AND CERVICAL SEGMENTS AND THEIR INVOLVEMENT IN NEUROPATHIC PAIN INDUCTION

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA, Marek JOUKAL et. al.

Základní údaje

Originální název

UPREGULATION OF IL-6 AND ITS SIGNALING MOLECULES IN THE DORSAL ROOT GANGLIA OF LUMBAR AND CERVICAL SEGMENTS AND THEIR INVOLVEMENT IN NEUROPATHIC PAIN INDUCTION

Název česky

Zvýšená syntéza IL-6 a jeho receptorů v lumbálních a cervikálních gangliích a jejich funkce při indukci neuropatické bolesti

Autoři

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA, Marek JOUKAL a Lucie KUBÍČKOVÁ

Vydání

4th International Congress on Neuropathic Pain, Toronto, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Kanada

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Středoevropský technologický institut

Klíčová slova česky

cytokiny, cytokinove recepory, spinální ganglion, neuropatická bolest

Klíčová slova anglicky

cytokines, cytokine receptors, dorsal root ganglia, neuropathic pain

Změněno: 23. 12. 2013 15:12, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

Background and aims. Interleukin-6 (IL-6) is a key component of the nervous system response to an injury with various effects. The aim of the present study was to investigate alterations of IL-6 and its signaling in both ipsilateral and contralateral L4-L5 as well as C7-C8 dorsal root ganglia (DRG) in rat neuropathic pain models. Methods. Unilateral chronic constriction (CCI) and spared nerve injury (SNI) of the sciatic nerve was performed aseptically in sixty rats. Neuropathic pain induction was tested by measurement of mechanoallodynia and thermal hyperalgesia. Expression of IL-6, IL-6R, gp130, STAT-3 and SOCS3 was investigated bilaterally in lumbar (L4-5) and cervical (C7-8) DRG 1, 3, 7 and 14 days after surgery. Some DRG sections were simultaneously immunostained for GAP-43. To prove a pathway for neuroinflammatory propagation, FluoroRuby was injected intrathecally at the level of L4-L5 spinal segments in six CCI-operated rats. Results. Ipsilateral hind paws of all rats operated on CCI/SNI displayed mechanoallodynia and thermal hyperalgesia while contralateral hind paws and forepaws of both sides exhibited no significant hypersensitivity. Bilateral elevation of IL-6 and its signal molecules was not limited to lumbar, but also extended to cervical DRG. FluoroRuby applied intrathecally diffused into both lumbar and cervical DRG. Conclusions. The results suggest that up-regulation of IL-6 and its signaling is not only associated with neuropathic pain induction. Neuroinflammatory reaction to neuropathic stress is propagated alongside neuroaxis from lumbar to remote DRG related probably with conditioning of the cervical DRG neurons to injury.

Česky

Cytokin IL-6 a jeho receptory a transformační faktor byly sysntetizovány ve spinálních gangliích a korespondovali s indukcíneuropatické bolesti.

Návaznosti

CZ.1.07/2.4.00/17.0042, interní kód MU

Název: InterBioNet - Podpora mezinárodní a mezisektorové spolupráce ve výzkumu a vývoji v oblasti věd o živé přírodě

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, InterBioNet - Podpora mezinárodní a mezisektorové spolupráce ve výzkumu a vývoji v oblasti věd o živé přírodě, 2.4 Partnerství a sítě

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA, Marek JOUKAL a Lucie KUBÍČKOVÁ. UPREGULATION OF IL-6 AND ITS SIGNALING MOLECULES IN THE DORSAL ROOT GANGLIA OF LUMBAR AND CERVICAL SEGMENTS AND THEIR INVOLVEMENT IN NEUROPATHIC PAIN INDUCTION. In 4th International Congress on Neuropathic Pain, Toronto. 2013.

@proceedings{1138016,
   author = {Dubový, Petr and Hradilová Svíženská, Ivana and Klusáková, Ilona and Brázda, Václav and Joukal, Marek and Kubíčková, Lucie},
   booktitle = {4th International Congress on Neuropathic Pain, Toronto},
   keywords = {cytokines, cytokine receptors, dorsal root ganglia, neuropathic pain},
   language = {eng},
   title = {UPREGULATION OF IL-6 AND ITS SIGNALING MOLECULES IN THE DORSAL ROOT GANGLIA OF LUMBAR AND CERVICAL SEGMENTS AND THEIR INVOLVEMENT IN NEUROPATHIC PAIN INDUCTION},
   year = {2013}
}

TY  - CONF
ID  - 1138016
AU  - Dubový, Petr - Hradilová Svíženská, Ivana - Klusáková, Ilona - Brázda, Václav - Joukal, Marek - Kubíčková, Lucie
PY  - 2013
TI  - UPREGULATION OF IL-6 AND ITS SIGNALING MOLECULES IN THE DORSAL ROOT GANGLIA OF LUMBAR AND CERVICAL SEGMENTS AND THEIR INVOLVEMENT IN NEUROPATHIC PAIN INDUCTION
KW  - cytokines, cytokine receptors, dorsal root ganglia, neuropathic pain
N2  - Background and aims. Interleukin-6 (IL-6) is a key component of the nervous system response to an injury with various effects. The aim of the present study was to investigate alterations of IL-6 and its signaling in both ipsilateral and contralateral L4-L5 as well as C7-C8 dorsal root ganglia (DRG) in rat neuropathic pain models. Methods. Unilateral chronic constriction (CCI) and spared nerve injury (SNI) of the sciatic nerve was performed aseptically in sixty rats. Neuropathic pain induction was tested by measurement of mechanoallodynia and thermal hyperalgesia. Expression of IL-6, IL-6R, gp130, STAT-3 and SOCS3 was investigated bilaterally in lumbar (L4-5) and cervical (C7-8) DRG 1, 3, 7 and 14 days after surgery. Some DRG sections were simultaneously immunostained for GAP-43. To prove a pathway for neuroinflammatory propagation, FluoroRuby was injected intrathecally at the level of L4-L5 spinal segments in six CCI-operated rats. Results. Ipsilateral hind paws of all rats operated on CCI/SNI displayed mechanoallodynia and thermal hyperalgesia while contralateral hind paws and forepaws of both sides exhibited no significant hypersensitivity. Bilateral elevation of IL-6 and its signal molecules was not limited to lumbar, but also extended to cervical DRG. FluoroRuby applied intrathecally diffused into both lumbar and cervical DRG. Conclusions. The results suggest that up-regulation of IL-6 and its signaling is not only associated with neuropathic pain induction. Neuroinflammatory reaction to neuropathic stress is propagated alongside neuroaxis from lumbar to remote DRG related probably with conditioning of the cervical DRG neurons to injury.
ER  -

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA, Marek JOUKAL a Lucie KUBÍČKOVÁ. UPREGULATION OF IL-6 AND ITS SIGNALING MOLECULES IN THE DORSAL ROOT GANGLIA OF LUMBAR AND CERVICAL SEGMENTS AND THEIR INVOLVEMENT IN NEUROPATHIC PAIN INDUCTION. In \textit{4th International Congress on Neuropathic Pain, Toronto}. 2013.

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