Following the mechanisms of bacteriostatic versus bactericidal
action using raman spectroscopy

J 2013

Following the mechanisms of bacteriostatic versus bactericidal action using raman spectroscopy

BERNATOVÁ, Silvie, Ota SAMEK, Zdeněk PILÁT, Mojmír ŠERÝ, Jan JEŽEK et. al.

Základní údaje

Originální název

Following the mechanisms of bacteriostatic versus bactericidal action using raman spectroscopy

Autoři

BERNATOVÁ, Silvie (203 Česká republika), Ota SAMEK (203 Česká republika), Zdeněk PILÁT (203 Česká republika), Mojmír ŠERÝ (203 Česká republika), Jan JEŽEK (203 Česká republika), Petr JÁKL (203 Česká republika), Martin ŠILER (203 Česká republika), Vladislav KRZYŽÁNEK (203 Česká republika), Pavel ZEMÁNEK (203 Česká republika), Veronika HOLÁ (203 Česká republika, domácí), Milada DVOŘÁČKOVÁ (203 Česká republika, domácí) a Filip RŮŽIČKA (203 Česká republika, garant, domácí)

Vydání

Molecules, Basel, Molecular Diversity Preservation International, 2013, 1420-3049

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.095

Kód RIV

RIV/00216224:14110/13:00066653

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/molecules181113188

UT WoS

000330311500005

Klíčová slova anglicky

Antibiotics; Bacteria; Bactericidal; Bacteriostatic; Raman spectroscopy

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 5. 2016 12:07, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Antibiotics cure infections by influencing bacterial growth or viability. Antibiotics can be divided to two groups on the basis of their effect on microbial cells through two main mechanisms, which are either bactericidal or bacteriostatic. Bactericidal antibiotics kill the bacteria and bacteriostatic antibiotics suppress the growth of bacteria (keep them in the stationary phase of growth). One of many factors to predict a favorable clinical outcome of the potential action of antimicrobial chemicals may be provided using in vitro bactericidal/bacteriostatic data (e.g., minimum inhibitory concentrations-MICs). Consequently, MICs are used in clinical situations mainly to confirm resistance, and to determine the in vitro activities of new antimicrobials. We report on the combination of data obtained from MICs with information on microorganisms' "fingerprint" (e.g., DNA/RNA, and proteins) provided by Raman spectroscopy. Thus, we could follow mechanisms of the bacteriostatic versus bactericidal action simply by detecting the Raman bands corresponding to DNA. The Raman spectra of Staphylococcus epidermidis treated with clindamycin (a bacteriostatic agent) indeed show little effect on DNA which is in contrast with the action of ciprofloxacin (a bactericidal agent), where the Raman spectra show a decrease in strength of the signal assigned to DNA, suggesting DNA fragmentation.

Návaznosti

GAP205/11/1687, projekt VaV

Název: Neinvazní bezkontaktní metody identifikace a charakterizace živých mikroorganismů prostřednictvím optické spektroskopie a mikromanipulace

Investor: Grantová agentura ČR, Neinvazní bezkontaktní metody identifikace a charakterizace živých mikroorganismů prostřednictvím optické spektroskopie a mikromanipulace

Citovat

BERNATOVÁ, Silvie, Ota SAMEK, Zdeněk PILÁT, Mojmír ŠERÝ, Jan JEŽEK, Petr JÁKL, Martin ŠILER, Vladislav KRZYŽÁNEK, Pavel ZEMÁNEK, Veronika HOLÁ, Milada DVOŘÁČKOVÁ a Filip RŮŽIČKA. Following the mechanisms of bacteriostatic versus bactericidal action using raman spectroscopy. Molecules. Basel: Molecular Diversity Preservation International, 2013, roč. 18, č. 11, s. 13188-13199. ISSN 1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules181113188.

@article{1138488,
   author = {Bernatová, Silvie and Samek, Ota and Pilát, Zdeněk and Šerý, Mojmír and Ježek, Jan and Jákl, Petr and Šiler, Martin and Krzyžánek, Vladislav and Zemánek, Pavel and Holá, Veronika and Dvořáčková, Milada and Růžička, Filip},
   article_location = {Basel},
   article_number = {11},
   doi = {http://dx.doi.org/10.3390/molecules181113188},
   keywords = {Antibiotics; Bacteria; Bactericidal; Bacteriostatic; Raman spectroscopy},
   language = {eng},
   issn = {1420-3049},
   journal = {Molecules},
   title = {Following the mechanisms of bacteriostatic versus bactericidal action using raman spectroscopy},
   volume = {18},
   year = {2013}
}

TY  - JOUR
ID  - 1138488
AU  - Bernatová, Silvie - Samek, Ota - Pilát, Zdeněk - Šerý, Mojmír - Ježek, Jan - Jákl, Petr - Šiler, Martin - Krzyžánek, Vladislav - Zemánek, Pavel - Holá, Veronika - Dvořáčková, Milada - Růžička, Filip
PY  - 2013
TI  - Following the mechanisms of bacteriostatic versus bactericidal action using raman spectroscopy
JF  - Molecules
VL  - 18
IS  - 11
SP  - 13188-13199
EP  - 13188-13199
PB  - Molecular Diversity Preservation International
SN  - 14203049
KW  - Antibiotics
KW  - Bacteria
KW  - Bactericidal
KW  - Bacteriostatic
KW  - Raman spectroscopy
N2  - Antibiotics cure infections by influencing bacterial growth or viability. Antibiotics can be divided to two groups on the basis of their effect on microbial cells through two main mechanisms, which are either bactericidal or bacteriostatic. Bactericidal antibiotics kill the bacteria and bacteriostatic antibiotics suppress the growth of bacteria (keep them in the stationary phase of growth). One of many factors to predict a favorable clinical outcome of the potential action of antimicrobial chemicals may be provided using in vitro bactericidal/bacteriostatic data (e.g., minimum inhibitory concentrations-MICs). Consequently, MICs are used in clinical situations mainly to confirm resistance, and to determine the in vitro activities of new antimicrobials. We report on the combination of data obtained from MICs with information on microorganisms' "fingerprint" (e.g., DNA/RNA, and proteins) provided by Raman spectroscopy. Thus, we could follow mechanisms of the bacteriostatic versus bactericidal action simply by detecting the Raman bands corresponding to DNA. The Raman spectra of Staphylococcus epidermidis treated with clindamycin (a bacteriostatic agent) indeed show little effect on DNA which is in contrast with the action of ciprofloxacin (a bactericidal agent), where the Raman spectra show a decrease in strength of the signal assigned to DNA, suggesting DNA fragmentation.
ER  -

BERNATOVÁ, Silvie, Ota SAMEK, Zdeněk PILÁT, Mojmír ŠERÝ, Jan JEŽEK, Petr JÁKL, Martin ŠILER, Vladislav KRZYŽÁNEK, Pavel ZEMÁNEK, Veronika HOLÁ, Milada DVOŘÁČKOVÁ a Filip RŮŽIČKA. Following the mechanisms of bacteriostatic versus bactericidal action using raman spectroscopy. \textit{Molecules}. Basel: Molecular Diversity Preservation International, 2013, roč.~18, č.~11, s.~13188-13199. ISSN~1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules181113188.

Podrobný výpis o publikaci