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@article{1138488, author = {Bernatová, Silvie and Samek, Ota and Pilát, Zdeněk and Šerý, Mojmír and Ježek, Jan and Jákl, Petr and Šiler, Martin and Krzyžánek, Vladislav and Zemánek, Pavel and Holá, Veronika and Dvořáčková, Milada and Růžička, Filip}, article_location = {Basel}, article_number = {11}, doi = {http://dx.doi.org/10.3390/molecules181113188}, keywords = {Antibiotics; Bacteria; Bactericidal; Bacteriostatic; Raman spectroscopy}, language = {eng}, issn = {1420-3049}, journal = {Molecules}, title = {Following the mechanisms of bacteriostatic versus bactericidal action using raman spectroscopy}, volume = {18}, year = {2013} }
TY - JOUR ID - 1138488 AU - Bernatová, Silvie - Samek, Ota - Pilát, Zdeněk - Šerý, Mojmír - Ježek, Jan - Jákl, Petr - Šiler, Martin - Krzyžánek, Vladislav - Zemánek, Pavel - Holá, Veronika - Dvořáčková, Milada - Růžička, Filip PY - 2013 TI - Following the mechanisms of bacteriostatic versus bactericidal action using raman spectroscopy JF - Molecules VL - 18 IS - 11 SP - 13188-13199 EP - 13188-13199 PB - Molecular Diversity Preservation International SN - 14203049 KW - Antibiotics KW - Bacteria KW - Bactericidal KW - Bacteriostatic KW - Raman spectroscopy N2 - Antibiotics cure infections by influencing bacterial growth or viability. Antibiotics can be divided to two groups on the basis of their effect on microbial cells through two main mechanisms, which are either bactericidal or bacteriostatic. Bactericidal antibiotics kill the bacteria and bacteriostatic antibiotics suppress the growth of bacteria (keep them in the stationary phase of growth). One of many factors to predict a favorable clinical outcome of the potential action of antimicrobial chemicals may be provided using in vitro bactericidal/bacteriostatic data (e.g., minimum inhibitory concentrations-MICs). Consequently, MICs are used in clinical situations mainly to confirm resistance, and to determine the in vitro activities of new antimicrobials. We report on the combination of data obtained from MICs with information on microorganisms' "fingerprint" (e.g., DNA/RNA, and proteins) provided by Raman spectroscopy. Thus, we could follow mechanisms of the bacteriostatic versus bactericidal action simply by detecting the Raman bands corresponding to DNA. The Raman spectra of Staphylococcus epidermidis treated with clindamycin (a bacteriostatic agent) indeed show little effect on DNA which is in contrast with the action of ciprofloxacin (a bactericidal agent), where the Raman spectra show a decrease in strength of the signal assigned to DNA, suggesting DNA fragmentation. ER -
BERNATOVÁ, Silvie, Ota SAMEK, Zdeněk PILÁT, Mojmír ŠERÝ, Jan JEŽEK, Petr JÁKL, Martin ŠILER, Vladislav KRZYŽÁNEK, Pavel ZEMÁNEK, Veronika HOLÁ, Milada DVOŘÁČKOVÁ a Filip RŮŽIČKA. Following the mechanisms of bacteriostatic versus bactericidal action using raman spectroscopy. \textit{Molecules}. Basel: Molecular Diversity Preservation International, 2013, roč.~18, č.~11, s.~13188-13199. ISSN~1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules181113188.
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