The role of ABC transporters associated with multidrug
resistance in stem cells

k 2013

The role of ABC transporters associated with multidrug resistance in stem cells

LÁNOVÁ, Martina, Josef VEČEŘA, Jan KUČERA, Jiřina MEDALOVÁ, Jiří PACHERNÍK et. al.

Základní údaje

Originální název

The role of ABC transporters associated with multidrug resistance in stem cells

Autoři

LÁNOVÁ, Martina (203 Česká republika, domácí), Josef VEČEŘA (203 Česká republika, domácí), Jan KUČERA (203 Česká republika, domácí), Jiřina MEDALOVÁ (203 Česká republika, domácí) a Jiří PACHERNÍK (203 Česká republika, garant, domácí)

Vydání

Stem Cells and Cell therapy: From research to modern clinical application, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30105 Physiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/13:00070749

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

ABC-transporters neural stem cells

Změněno: 11. 3. 2015 10:51, RNDr. Josef Večeřa, Ph.D.

Anotace

V originále

ATP-binding transporters (ABC-t) play various roles in regulation of organism function and homeostasis from prokaryote to mammals. ABC-t mediate transport mainly of lipophilic substances through cellular membranes. These transporters protect stem cells against toxic substances of either endogenous, or importantly, exogenous origin. These transporters are called ABC-t associated with multi-drug resistance (MDR), according to their role in multidrug resistance to pharmacotherapy. High level of ABC-t expression is a typical feature of stem cells and many types of cancer stem cells. Particularly, ABCB1/P-glycoprotein/MDR1, ABCC1/multidrug resistance-associated protein 1 (MRP1), and ABCG2/BCRP (Breast cancer resistance protein);. However, substrates of ABC-t/MDR are not only toxins, but also important signaling molecules as well as leukotrienes and/or glutathione conjugates; and porphyrins, which mediate balance in intracellular oxidation-reduction processing. Thus we hypothesize the role of ABC-t/MDR in regulation of stem cells fate. To test this hypothesis we analyzed effect of modulation of ABC-t/MDR activity in embryonic and neural stem cells. We observed that ABCC1 and ABCG2 are the most expressed ABC-t/MDR in our tested stem cells. Importantly, inhibition of these ABC-t/MDR leads to decreasing of stemness and induction of differentiation in both embryonic and neural stem cells. Analysis of mechanism of observed effect and identification of studying ABC-t/MDR substrates, which may be responsible for this effect, are in progress.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

Citovat

LÁNOVÁ, Martina, Josef VEČEŘA, Jan KUČERA, Jiřina MEDALOVÁ a Jiří PACHERNÍK. The role of ABC transporters associated with multidrug resistance in stem cells. In Stem Cells and Cell therapy: From research to modern clinical application. 2013.

@proceedings{1138588,
   author = {Lánová, Martina and Večeřa, Josef and Kučera, Jan and Medalová, Jiřina and Pacherník, Jiří},
   booktitle = {Stem Cells and Cell therapy: From research to modern clinical application},
   keywords = {ABC-transporters neural stem cells},
   language = {eng},
   title = {The role of ABC transporters associated with multidrug resistance in stem cells},
   url = {http://www.symma.cz/baria/down/abstrakta_2013.pdf},
   year = {2013}
}

TY  - CONF
ID  - 1138588
AU  - Lánová, Martina - Večeřa, Josef - Kučera, Jan - Medalová, Jiřina - Pacherník, Jiří
PY  - 2013
TI  - The role of ABC transporters associated with multidrug resistance in stem cells
KW  - ABC-transporters neural stem cells
UR  - http://www.symma.cz/baria/down/abstrakta_2013.pdf
L2  - http://www.symma.cz/baria/down/abstrakta_2013.pdf
N2  - ATP-binding transporters (ABC-t) play various roles in regulation of organism function and homeostasis from prokaryote to mammals. ABC-t mediate transport mainly of lipophilic substances through cellular membranes. These transporters protect stem cells against toxic substances of either endogenous, or importantly, exogenous origin. These transporters are called ABC-t associated with multi-drug resistance (MDR), according to their role in multidrug resistance to pharmacotherapy. High level of ABC-t expression is a typical feature of stem cells and many types of cancer stem cells. Particularly, ABCB1/P-glycoprotein/MDR1, ABCC1/multidrug resistance-associated protein 1 (MRP1), and ABCG2/BCRP (Breast cancer resistance protein);. However, substrates of ABC-t/MDR are not only toxins, but also important signaling molecules as well as leukotrienes and/or glutathione conjugates; and porphyrins, which mediate balance in intracellular oxidation-reduction processing. Thus we hypothesize the role of ABC-t/MDR in regulation of stem cells fate. To test this hypothesis we analyzed effect of modulation of ABC-t/MDR activity in embryonic and neural stem cells. We observed that ABCC1 and ABCG2 are the most expressed ABC-t/MDR in our tested stem cells. Importantly, inhibition of these ABC-t/MDR leads to decreasing of stemness and induction of differentiation in both embryonic and neural stem cells. Analysis of mechanism of observed effect and identification of studying ABC-t/MDR substrates, which may be responsible for this effect, are in progress.
ER  -

LÁNOVÁ, Martina, Josef VEČEŘA, Jan KUČERA, Jiřina MEDALOVÁ a Jiří PACHERNÍK. The role of ABC transporters associated with multidrug resistance in stem cells. In \textit{Stem Cells and Cell therapy: From research to modern clinical application}. 2013.

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