Clonal evolution in chronic lymphocytic leukemia detected by
fluorescence in situ hybridization and conventional
cytogenetics after stimulation with CpG oligonucleotides and
interleukin-2: A prospective analysis.

J 2014

Clonal evolution in chronic lymphocytic leukemia detected by fluorescence in situ hybridization and conventional cytogenetics after stimulation with CpG oligonucleotides and interleukin-2: A prospective analysis.

BREJCHA, Martin, Martina STOKLASOVÁ, Yvona BRYCHTOVÁ, Anna PANOVSKÁ, Kristina ŠTĚPANOVSKÁ et. al.

Základní údaje

Originální název

Clonal evolution in chronic lymphocytic leukemia detected by fluorescence in situ hybridization and conventional cytogenetics after stimulation with CpG oligonucleotides and interleukin-2: A prospective analysis.

Autoři

BREJCHA, Martin (203 Česká republika), Martina STOKLASOVÁ (203 Česká republika), Yvona BRYCHTOVÁ (203 Česká republika, domácí), Anna PANOVSKÁ (203 Česká republika, domácí), Kristina ŠTĚPANOVSKÁ (203 Česká republika, domácí), Gabriela VAŇKOVÁ (203 Česká republika, domácí), Karla PLEVOVÁ (203 Česká republika, domácí), Alexandra OLTOVÁ (203 Česká republika, domácí), Kateřina HORKÁ (203 Česká republika), Šárka POSPÍŠILOVÁ (203 Česká republika, garant, domácí), Jiří MAYER (203 Česká republika, domácí) a Michael DOUBEK (203 Česká republika, domácí)

Vydání

Leukemia Research, Oxford, Pergamon Elsevier, 2014, 0145-2126

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.351

Kód RIV

RIV/00216224:14740/14:00074868

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.leukres.2013.10.019

UT WoS

000329971200007

EID Scopus

2-s2.0-84892526647

Klíčová slova anglicky

Chronic lymphocytic leukemia; Clonal evolution; CpG oligonucleotides; Cytogenetics; Fluorescence in situ hybridization; Interleukin-2

Štítky

EL OK, kontrola MP, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 4. 2015 11:03, Martina Prášilová

Anotace

V originále

Chronic lymphocytic leukemia (CLL) patients may acquire new chromosome abnormalities during the course of their disease. Clonal evolution (CE) has been detected by conventional chromosome banding (CBA), several groups also confirmed CE with fluorescence in situ hybridization (FISH). At present, there are minimal prospective data on CE frequency determined using a combination of both methods. Therefore, the aim of our study was to prospectively assess CE frequency using a combination of FISH and CBA after stimulation with CpG oligonucleotides and interleukin-2. Between 2008 and 2012, we enrolled 140 patients with previously untreated CLL in a prospective trial evaluating CE using FISH and CBA after stimulation. Patients provided baseline and regular follow-up peripheral blood samples for testing. There was a median of 3 cytogenetic examinations (using both methods) per patient. CE was detected in 15.7% (22/140) of patients using FISH, in 28.6% (40/140) using CBA, and in 34.3% (48/140) of patients by combining both methods. Poor-prognosis CE (new deletion 17p, new deletion 11q or new complex karyotype) was detected in 15% (21/140) of patients and was significantly associated with previous CLL treatment (p=0.013). CBA provides more complex information about cytogenetic abnormalities in CLL patients than FISH and confirms that many patients can acquire new abnormalities during the course of their disease in a relatively short time period.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

EE2.3.20.0045, projekt VaV

Název: Podpora profesního růstu a mezinárodní integrace výzkumných týmů v oblasti molekulární medicíny

MSM0021622430, záměr

Název: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

MUNI/A/0723/2012, interní kód MU

Název: Nové klinické a diagnostické přístupy u hematogických malignit (Akronym: NoKliDiPřiHeMa)

Investor: Masarykova univerzita, Nové klinické a diagnostické přístupy u hematogických malignit, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

NT13493, projekt VaV

Název: Molekulární charakterizace B buněčných receptorů a jejich vztah k evoluci genetických změn u chronické lymfocytární leukémie

7E13008, projekt VaV

Název: Next Generation Sequencing Platform for Targeted Personalized Therapy of Leukemia (Akronym: NGS-PTL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Next Generation Sequencing Platform for Targeted Personalized Therapy of Leukemia

Přiložené soubory

Brejcha.pdf

Požádat o autorskou verzi souboru

EL_1138698_Clonal_evolution.pdf

Požádat o autorskou verzi souboru

Citovat

BREJCHA, Martin, Martina STOKLASOVÁ, Yvona BRYCHTOVÁ, Anna PANOVSKÁ, Kristina ŠTĚPANOVSKÁ, Gabriela VAŇKOVÁ, Karla PLEVOVÁ, Alexandra OLTOVÁ, Kateřina HORKÁ, Šárka POSPÍŠILOVÁ, Jiří MAYER a Michael DOUBEK. Clonal evolution in chronic lymphocytic leukemia detected by fluorescence in situ hybridization and conventional cytogenetics after stimulation with CpG oligonucleotides and interleukin-2: A prospective analysis. Leukemia Research. Oxford: Pergamon Elsevier, 2014, roč. 38, č. 2, s. 170-175. ISSN 0145-2126. Dostupné z: https://dx.doi.org/10.1016/j.leukres.2013.10.019.

@article{1138698,
   author = {Brejcha, Martin and Stoklasová, Martina and Brychtová, Yvona and Panovská, Anna and Štěpanovská, Kristina and Vaňková, Gabriela and Plevová, Karla and Oltová, Alexandra and Horká, Kateřina and Pospíšilová, Šárka and Mayer, Jiří and Doubek, Michael},
   article_location = {Oxford},
   article_number = {2},
   doi = {http://dx.doi.org/10.1016/j.leukres.2013.10.019},
   keywords = {Chronic lymphocytic leukemia; Clonal evolution; CpG oligonucleotides; Cytogenetics; Fluorescence in situ hybridization; Interleukin-2},
   language = {eng},
   issn = {0145-2126},
   journal = {Leukemia Research},
   title = {Clonal evolution in chronic lymphocytic leukemia detected by fluorescence in situ hybridization and conventional cytogenetics after stimulation with CpG oligonucleotides and interleukin-2: A prospective analysis.},
   url = {http://ac.els-cdn.com/S0145212613003743/1-s2.0-S0145212613003743-main.pdf?_tid=94685418-c7f7-11e4-a9b3-00000aacb362&acdnat=1426082868_d96dbfd19537a9a8ff20a6f2dd3b991a},
   volume = {38},
   year = {2014}
}

TY  - JOUR
ID  - 1138698
AU  - Brejcha, Martin - Stoklasová, Martina - Brychtová, Yvona - Panovská, Anna - Štěpanovská, Kristina - Vaňková, Gabriela - Plevová, Karla - Oltová, Alexandra - Horká, Kateřina - Pospíšilová, Šárka - Mayer, Jiří - Doubek, Michael
PY  - 2014
TI  - Clonal evolution in chronic lymphocytic leukemia detected by fluorescence in situ hybridization and conventional cytogenetics after stimulation with CpG oligonucleotides and interleukin-2: A prospective analysis.
JF  - Leukemia Research
VL  - 38
IS  - 2
SP  - 170-175
EP  - 170-175
PB  - Pergamon Elsevier
SN  - 01452126
KW  - Chronic lymphocytic leukemia
KW  - Clonal evolution
KW  - CpG oligonucleotides
KW  - Cytogenetics
KW  - Fluorescence in situ hybridization
KW  - Interleukin-2
UR  - http://ac.els-cdn.com/S0145212613003743/1-s2.0-S0145212613003743-main.pdf?_tid=94685418-c7f7-11e4-a9b3-00000aacb362&acdnat=1426082868_d96dbfd19537a9a8ff20a6f2dd3b991a
L2  - http://ac.els-cdn.com/S0145212613003743/1-s2.0-S0145212613003743-main.pdf?_tid=94685418-c7f7-11e4-a9b3-00000aacb362&acdnat=1426082868_d96dbfd19537a9a8ff20a6f2dd3b991a
N2  - Chronic lymphocytic leukemia (CLL) patients may acquire new chromosome abnormalities during the course of their disease. Clonal evolution (CE) has been detected by conventional chromosome banding (CBA), several groups also confirmed CE with fluorescence in situ hybridization (FISH). At present, there are minimal prospective data on CE frequency determined using a combination of both methods. Therefore, the aim of our study was to prospectively assess CE frequency using a combination of FISH and CBA after stimulation with CpG oligonucleotides and interleukin-2. Between 2008 and 2012, we enrolled 140 patients with previously untreated CLL in a prospective trial evaluating CE using FISH and CBA after stimulation. Patients provided baseline and regular follow-up peripheral blood samples for testing. There was a median of 3 cytogenetic examinations (using both methods) per patient. CE was detected in 15.7% (22/140) of patients using FISH, in 28.6% (40/140) using CBA, and in 34.3% (48/140) of patients by combining both methods. Poor-prognosis CE (new deletion 17p, new deletion 11q or new complex karyotype) was detected in 15% (21/140) of patients and was significantly associated with previous CLL treatment (p=0.013). CBA provides more complex information about cytogenetic abnormalities in CLL patients than FISH and confirms that many patients can acquire new abnormalities during the course of their disease in a relatively short time period.
ER  -

BREJCHA, Martin, Martina STOKLASOVÁ, Yvona BRYCHTOVÁ, Anna PANOVSKÁ, Kristina ŠTĚPANOVSKÁ, Gabriela VAŇKOVÁ, Karla PLEVOVÁ, Alexandra OLTOVÁ, Kateřina HORKÁ, Šárka POSPÍŠILOVÁ, Jiří MAYER a Michael DOUBEK. Clonal evolution in chronic lymphocytic leukemia detected by fluorescence in situ hybridization and conventional cytogenetics after stimulation with CpG oligonucleotides and interleukin-2: A prospective analysis. \textit{Leukemia Research}. Oxford: Pergamon Elsevier, 2014, roč.~38, č.~2, s.~170-175. ISSN~0145-2126. Dostupné z: https://dx.doi.org/10.1016/j.leukres.2013.10.019.

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