Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

VESELSKÁ, Renata, Jakub NERADIL, Marta NEKULOVÁ, Lucia DOBRUCKÁ, Bořivoj VOJTĚŠEK, Jaroslav ŠTĚRBA and Karel ZITTERBART. Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies. Histology and Histopathology. 2013, vol. 28, No 7, p. 913-924. ISSN 0213-3911.

Basic information

• Original name: Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies
• Authors: VESELSKÁ, Renata (203 Czech Republic, guarantor, belonging to the institution), Jakub NERADIL (203 Czech Republic, belonging to the institution), Marta NEKULOVÁ (203 Czech Republic, belonging to the institution), Lucia DOBRUCKÁ (703 Slovakia, belonging to the institution), Bořivoj VOJTĚŠEK (203 Czech Republic), Jaroslav ŠTĚRBA (203 Czech Republic, belonging to the institution) and Karel ZITTERBART (203 Czech Republic, belonging to the institution).
• Edition: Histology and Histopathology, 2013, 0213-3911.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: Spain
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.236
• RIV identification code: RIV/00216224:14310/13:00065656
• Organization unit: Faculty of Science
• UT WoS: 000320710900011
• Keywords in English: medulloblastoma;deltaNp73;immunocytochemistry;immunoblotting;transmission electron microscopy
• Tags: AKR, podil, rivok
• Tags: International impact, Reviewed

Abstract

This study is focused on the intracellular localization of Delta Np73 in medulloblastoma cell lines. Due to a lack of suitable anti-Delta Np73 antibodies, we developed two new rabbit polyclonal antibodies, Delta Np7326 and Delta Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the Delta Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the Delta Np73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the Delta Np73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with Delta Np73 and golgin-97, we showed the co-localization of the Delta Np73 isoform with the Golgi apparatus.

Links

• NS10218, research and development project: Name: Role izoforem proteinu p73 v patogenezi meduloblastomu: imunocytochemická a microarray studie

Investor: Ministry of Health of the CR