2013
The association between the expression of solute carrier transporters and the prognosis of pancreatic cancer
MOHELNIKOVA-DUCHONOVA, Beatrice, Veronika BRYNYCHOVA, Viktor HLAVAC, Matej KOCIK, Martin OLIVERIUS et. al.Základní údaje
Originální název
The association between the expression of solute carrier transporters and the prognosis of pancreatic cancer
Autoři
MOHELNIKOVA-DUCHONOVA, Beatrice (203 Česká republika), Veronika BRYNYCHOVA (203 Česká republika), Viktor HLAVAC (203 Česká republika), Matej KOCIK (203 Česká republika), Martin OLIVERIUS (203 Česká republika), Jan HLAVSA (203 Česká republika, garant, domácí), Eva HONSOVA (203 Česká republika), Jan MAZANEC (203 Česká republika, domácí), Zdeněk KALA (203 Česká republika, domácí), Bohuslav MELICHAR (203 Česká republika) a Pavel SOUCEK (203 Česká republika)
Vydání
CANCER CHEMOTHERAPY AND PHARMACOLOGY, NEW YORK, SPRINGER, 2013, 0344-5704
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30200 3.2 Clinical medicine
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.571
Kód RIV
RIV/00216224:14110/13:00071472
Organizační jednotka
Lékařská fakulta
UT WoS
000323653600019
Klíčová slova anglicky
Pancreatic cancer; SLC transporters; Gene expression; Survival; KRAS
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 3. 2. 2014 13:32, Soňa Böhmová
Anotace
V originále
The aim of this study was to investigate the prognostic significance of fourteen anticancer drug-relevant solute carrier transporters (SLCs) in pancreatic cancer in the context of clinical-pathological characteristics and the KRAS mutation status of tumors. Tumors and non-neoplastic pancreatic tissues were obtained from 32 histologically verified patients with pancreatic ductal adenocarcinoma. The transcript profile of SLCs was assessed using quantitative real-time PCR. KRAS mutations in exon 2 were assessed by high-resolution melting analysis and confirmed by sequencing. SLC22A3 and SLC22A18 were upregulated and SLC22A1, SLC22A2, SLC22A11, SLC28A1, SLC28A3 and SLC29A1 were downregulated when compared with non-neoplastic pancreatic tissues. Moreover, significantly lower levels of SLC22A1, SLC22A11 and SLC29A1 were found in tumors with angioinvasion. There was also a significantly higher transcript level of SLC28A1 in tumors with regional lymph nodes affected by metastasis. The study found that a high expression of SLC28A1 was significantly associated with poor overall survival in unselected patients. In contrast, a high expression of SLC22A3 or SLC29A3 was significantly associated with longer overall survival in patients treated with nucleoside analogs. Protein expression of SLC22A1, SLC22A3 and SLC29A3 in tumor tissues of patients with pancreatic carcinoma was observed by immunoblotting for the first time. Finally, SLC levels were not found to be associated with KRAS mutation status in exon 2. This study identified a number of associations of transcript levels of SLCs with prognosis of pancreatic cancer patients.