2013
The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria
BLÁHOVÁ, Lucie, Ondřej ADAMOVSKÝ, Lukáš KUBALA, Lenka SINDLEROVA SVIHALKOVA, Radka ZOUNKOVÁ et. al.Základní údaje
Originální název
The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria
Autoři
BLÁHOVÁ, Lucie (203 Česká republika, domácí), Ondřej ADAMOVSKÝ (203 Česká republika, domácí), Lukáš KUBALA (203 Česká republika), Lenka SINDLEROVA SVIHALKOVA (203 Česká republika), Radka ZOUNKOVÁ (203 Česká republika, domácí) a Luděk BLÁHA (203 Česká republika, garant, domácí)
Vydání
Toxicon, OXFORD, Elsevier Science, 2013, 0041-0101
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10511 Environmental sciences
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.581
Kód RIV
RIV/00216224:14310/13:00066817
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000328658600023
Klíčová slova anglicky
Endotoxin; Cyanobacteria; Water bloom; Lipopolysaccharide; Microcystis
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 9. 4. 2014 17:28, Ing. Andrea Mikešková
Anotace
V originále
Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide, yet serious gaps exist in understanding their complex toxic effects, including the role of lipopolysaccharides (LPS). The present comparative study focused on the levels and biological activities of LPS isolated from Microcystis aeruginosa, which is one of the most globally distributed toxic species. Using hot phenol extraction, LPS was isolated from 3 laboratory cultures and 11 natural water blooms. It formed 0.2-0.7% of the original dry biomass of the cyanobacteria, based on gravimetry. Additional analyses by commercial anti-LPS ELISA were correlated with gravimetry but showed concentrations that were about 7-times lower, which indicated either impurities in isolated LPS or the poor cross-reactivity of the antibodies used. LPS isolates from M. aeruginosa were potent pyrogens in the traditional Limulus amebocyte lysate (LAL)-test, but comparison with the PyroGene test demonstrated the limited selectivity of LAL with several interferences. The determined pyrogenicity (endotoxin units, EU) ranged from very low values in laboratory cultures (less than 0.003 up to 0.008-EU per 100 pg LPS) to higher values in complex bloom samples (0.01-0.078 EU per 100 pg of LPS), which suggested the role of bloom-associated bacteria in the overall effects. Potent pro-inflammatory effects of the studied LPS from both cultures and bloom samples were observed in a highly-relevant ex vivo human blood model by studying reactive oxygen species production in phagocytes as well as increased productions of interleukin 8, IL-8, and tumor necrosis factor alpha, TNF-alpha. LPS from M aeruginosa seem to modulate several pathways involved in the regulation of both innate immunity and specific responses.
Návaznosti
ED0001/01/01, projekt VaV |
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GP13-27695P, projekt VaV |
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