Stability of human telomere quadruplexes at high DNA
concentrations

J 2014

Stability of human telomere quadruplexes at high DNA concentrations

KEJNOVSKÁ, Iva, Michaela VORLÍČKOVÁ, Marie BRÁZDOVÁ a Janos SAGI

Základní údaje

Originální název

Stability of human telomere quadruplexes at high DNA concentrations

Autoři

KEJNOVSKÁ, Iva (203 Česká republika), Michaela VORLÍČKOVÁ (203 Česká republika, garant, domácí), Marie BRÁZDOVÁ (203 Česká republika) a Janos SAGI (840 Spojené státy)

Vydání

Biopolymers, Hoboken, WILEY-BLACKWELL, 2014, 0006-3525

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.385

Kód RIV

RIV/00216224:14740/14:00074985

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1002/bip.22400

UT WoS

000336575300013

Klíčová slova anglicky

DNA concentration; circular dichroism; folding topology; quadruplex; thermal and thermodynamic stability

Štítky

kontrola MP, ok, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 10. 2014 17:58, Martina Prášilová

Anotace

V originále

For mimicking macromolecular crowding of DNA quadruplexes, various crowding agents have been used, typically PEG, with quadruplexes of micromolar strand concentrations. Thermal and thermodynamic stabilities of these quadruplexes increased with the concentration of the agents, the rise depended on the crowder used. A different phenomenon was observed, and is presented in this article, when the crowder was the quadruplex itself. With DNA strand concentrations ranging from 3 mM to 9 mM, the thermostability did not change up to 2 mM, above which it increased, indicating that the unfolding quadruplex units were not monomolecular above 2 mM. The results are explained by self-association of the G-quadruplexes above this concentration. The deltaG(o) 37 values, evaluated only below 2 mM, did not become more negative, as with the non-DNA crowders, instead, slightly increased. Folding topology changed from antiparallel to hybrid above 2 mM, and then to parallel quadruplexes at high, 6-9 mM strand concentrations. In this range, the concentration of the DNA phosphate anions approached the concentration of the K(+) counterions used. Volume exclusion is assumed to promote the topological changes of quadruplexes toward the parallel, and the decreased screening of anions could affect their stability.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

KEJNOVSKÁ, Iva, Michaela VORLÍČKOVÁ, Marie BRÁZDOVÁ a Janos SAGI. Stability of human telomere quadruplexes at high DNA concentrations. Biopolymers. Hoboken: WILEY-BLACKWELL, 2014, roč. 101, č. 4, s. 428-438. ISSN 0006-3525. Dostupné z: https://dx.doi.org/10.1002/bip.22400.

@article{1161956,
   author = {Kejnovská, Iva and Vorlíčková, Michaela and Brázdová, Marie and Sagi, Janos},
   article_location = {Hoboken},
   article_number = {4},
   doi = {http://dx.doi.org/10.1002/bip.22400},
   keywords = {DNA concentration; circular dichroism; folding topology; quadruplex; thermal and thermodynamic stability},
   language = {eng},
   issn = {0006-3525},
   journal = {Biopolymers},
   title = {Stability of human telomere quadruplexes at high DNA concentrations},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/bip.22400/abstract},
   volume = {101},
   year = {2014}
}

TY  - JOUR
ID  - 1161956
AU  - Kejnovská, Iva - Vorlíčková, Michaela - Brázdová, Marie - Sagi, Janos
PY  - 2014
TI  - Stability of human telomere quadruplexes at high DNA concentrations
JF  - Biopolymers
VL  - 101
IS  - 4
SP  - 428-438
EP  - 428-438
PB  - WILEY-BLACKWELL
SN  - 00063525
KW  - DNA concentration
KW  - circular dichroism
KW  - folding topology
KW  - quadruplex
KW  - thermal and thermodynamic stability
UR  - http://onlinelibrary.wiley.com/doi/10.1002/bip.22400/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/bip.22400/abstract
N2  - For mimicking macromolecular crowding of DNA quadruplexes, various crowding agents have been used, typically PEG, with quadruplexes of micromolar strand concentrations. Thermal and thermodynamic stabilities of these quadruplexes increased with the concentration of the agents, the rise depended on the crowder used. A different phenomenon was observed, and is presented in this article, when the crowder was the quadruplex itself. With DNA strand concentrations ranging from 3 mM to 9 mM, the thermostability did not change up to 2 mM, above which it increased, indicating that the unfolding quadruplex units were not monomolecular above 2 mM. The results are explained by self-association of the G-quadruplexes above this concentration. The deltaG(o) 37 values, evaluated only below 2 mM, did not become more negative, as with the non-DNA crowders, instead, slightly increased. Folding topology changed from antiparallel to hybrid above 2 mM, and then to parallel quadruplexes at high, 6-9 mM strand concentrations. In this range, the concentration of the DNA phosphate anions approached the concentration of the K(+) counterions used. Volume exclusion is assumed to promote the topological changes of quadruplexes toward the parallel, and the decreased screening of anions could affect their stability.
ER  -

KEJNOVSKÁ, Iva, Michaela VORLÍČKOVÁ, Marie BRÁZDOVÁ a Janos SAGI. Stability of human telomere quadruplexes at high DNA concentrations. \textit{Biopolymers}. Hoboken: WILEY-BLACKWELL, 2014, roč.~101, č.~4, s.~428-438. ISSN~0006-3525. Dostupné z: https://dx.doi.org/10.1002/bip.22400.

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