Preferential Binding of Hot Spot Mutant p53 Proteins to
Supercoiled DNA In Vitro and in Cells

J 2013

Preferential Binding of Hot Spot Mutant p53 Proteins to Supercoiled DNA In Vitro and in Cells

BRÁZDOVÁ, Marie, Lucie NAVRATILOVA, Vlastimil TICHÝ, Kateřina NĚMCOVÁ, Matej LEXA et. al.

Základní údaje

Originální název

Preferential Binding of Hot Spot Mutant p53 Proteins to Supercoiled DNA In Vitro and in Cells

Autoři

BRÁZDOVÁ, Marie (203 Česká republika), Lucie NAVRATILOVA (203 Česká republika), Vlastimil TICHÝ (203 Česká republika, garant, domácí), Kateřina NĚMCOVÁ (203 Česká republika), Matej LEXA (703 Slovensko, domácí), Roman HRSTKA (203 Česká republika), Petr PECINKA (203 Česká republika), Matej ADÁMIK (703 Slovensko, domácí), Bořivoj VOJTĚŠEK (203 Česká republika), Emil PALECEK (203 Česká republika), Wolfgang DEPPERT (276 Německo) a Miroslav FOJTA (203 Česká republika, domácí)

Vydání

Plos One, SAN FRANCISCO, PUBLIC LIBRARY SCIENCE, 2013, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Brazdova et al. 2013

Impakt faktor

Impact factor: 3.534

Kód RIV

RIV/00216224:14330/13:00066842

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1371/journal.pone.0059567

UT WoS

000317418500055

Klíčová slova anglicky

p53 tumor supressor; mutp53 oncogenic mutations; DNA topology; superhelicity; DNA binding; BAX MSP/MST1 promoter

Změněno: 13. 3. 2018 14:20, doc. Ing. Matej Lexa, Ph.D.

Anotace

V originále

Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding of mutp53 to DNA is assumed to be involved in mutp53-mediated repression or activation of several mutp53 target genes. To investigate the importance of DNA topology on mutp53-DNA recognition in vitro and in cells, we analyzed the interaction of seven hot spot mutp53 proteins with topologically different DNA substrates (supercoiled, linear and relaxed) containing and/or lacking mutp53 binding sites (mutp53BS) using a variety of electrophoresis and immunoprecipitation based techniques. All seven hot spot mutp53 proteins (R175H, G245S, R248W, R249S, R273C, R273H and R282W) were found to have retained the ability of wild-type p53 to preferentially bind circular DNA at native negative superhelix density, while linear or relaxed circular DNA was a poor substrate. The preference of mutp53 proteins for supercoiled DNA (supercoil-selective binding) was further substantiated by competition experiments with linear DNA or relaxed DNA in vitro and ex vivo. Using chromatin immunoprecipitation, the preferential binding of mutp53 to a sc mutp53BS was detected also in cells. Furthermore, we have shown by luciferase reporter assay that the DNA topology influences p53 regulation of BAX and MSP/MST1 promoters. Possible modes of mutp53 binding to topologically constrained DNA substrates and their biological consequences are discussed.

Návaznosti

GA204/08/1560, projekt VaV

Název: Bioinformatická a experimentální identifikace nekanonických struktur v genomové DNA

Investor: Grantová agentura ČR, Bioinformatická a experimentální identifikace nekanonických struktur v genomové DNA

Citovat

BRÁZDOVÁ, Marie, Lucie NAVRATILOVA, Vlastimil TICHÝ, Kateřina NĚMCOVÁ, Matej LEXA, Roman HRSTKA, Petr PECINKA, Matej ADÁMIK, Bořivoj VOJTĚŠEK, Emil PALECEK, Wolfgang DEPPERT a Miroslav FOJTA. Preferential Binding of Hot Spot Mutant p53 Proteins to Supercoiled DNA In Vitro and in Cells. Plos One. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2013, roč. 8, č. 3, s. "e59567", 17 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0059567.

@article{1163356,
   author = {Brázdová, Marie and Navratilova, Lucie and Tichý, Vlastimil and Němcová, Kateřina and Lexa, Matej and Hrstka, Roman and Pecinka, Petr and Adámik, Matej and Vojtěšek, Bořivoj and Palecek, Emil and Deppert, Wolfgang and Fojta, Miroslav},
   article_location = {SAN FRANCISCO},
   article_number = {3},
   doi = {http://dx.doi.org/10.1371/journal.pone.0059567},
   keywords = {p53 tumor supressor; mutp53 oncogenic mutations; DNA topology; superhelicity; DNA binding; BAX MSP/MST1 promoter},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos One},
   title = {Preferential Binding of Hot Spot Mutant p53 Proteins to Supercoiled DNA In Vitro and in Cells},
   url = {http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0059567},
   volume = {8},
   year = {2013}
}

TY  - JOUR
ID  - 1163356
AU  - Brázdová, Marie - Navratilova, Lucie - Tichý, Vlastimil - Němcová, Kateřina - Lexa, Matej - Hrstka, Roman - Pecinka, Petr - Adámik, Matej - Vojtěšek, Bořivoj - Palecek, Emil - Deppert, Wolfgang - Fojta, Miroslav
PY  - 2013
TI  - Preferential Binding of Hot Spot Mutant p53 Proteins to Supercoiled DNA In Vitro and in Cells
JF  - Plos One
VL  - 8
IS  - 3
SP  - "e59567"
EP  - "e59567"
PB  - PUBLIC LIBRARY SCIENCE
SN  - 19326203
KW  - p53 tumor supressor
KW  - mutp53 oncogenic mutations
KW  - DNA topology
KW  - superhelicity
KW  - DNA binding
KW  - BAX MSP/MST1 promoter
UR  - http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0059567
L2  - http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0059567
N2  - Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding of mutp53 to DNA is assumed to be involved in mutp53-mediated repression or activation of several mutp53 target genes. To investigate the importance of DNA topology on mutp53-DNA recognition in vitro and in cells, we analyzed the interaction of seven hot spot mutp53 proteins with topologically different DNA substrates (supercoiled, linear and relaxed) containing and/or lacking mutp53 binding sites (mutp53BS) using a variety of electrophoresis and immunoprecipitation based techniques. All seven hot spot mutp53 proteins (R175H, G245S, R248W, R249S, R273C, R273H and R282W) were found to have retained the ability of wild-type p53 to preferentially bind circular DNA at native negative superhelix density, while linear or relaxed circular DNA was a poor substrate. The preference of mutp53 proteins for supercoiled DNA (supercoil-selective binding) was further substantiated by competition experiments with linear DNA or relaxed DNA in vitro and ex vivo. Using chromatin immunoprecipitation, the preferential binding of mutp53 to a sc mutp53BS was detected also in cells. Furthermore, we have shown by luciferase reporter assay that the DNA topology influences p53 regulation of BAX and MSP/MST1 promoters. Possible modes of mutp53 binding to topologically constrained DNA substrates and their biological consequences are discussed.
ER  -

BRÁZDOVÁ, Marie, Lucie NAVRATILOVA, Vlastimil TICHÝ, Kateřina NĚMCOVÁ, Matej LEXA, Roman HRSTKA, Petr PECINKA, Matej ADÁMIK, Bořivoj VOJTĚŠEK, Emil PALECEK, Wolfgang DEPPERT a Miroslav FOJTA. Preferential Binding of Hot Spot Mutant p53 Proteins to Supercoiled DNA In Vitro and in Cells. \textit{Plos One}. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2013, roč.~8, č.~3, s.~''e59567'', 17 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0059567.

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