MALDI MS and ICP MS Detection of a Single CE Separation Record:
A Tool for Metalloproteomics

J 2014

MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics

TOMALOVÁ, Iva, Pavla FOLTYNOVÁ, Viktor KANICKÝ a Jan PREISLER

Základní údaje

Originální název

MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics

Autoři

TOMALOVÁ, Iva (203 Česká republika, domácí), Pavla FOLTYNOVÁ (203 Česká republika, domácí), Viktor KANICKÝ (203 Česká republika, domácí) a Jan PREISLER (203 Česká republika, garant, domácí)

Vydání

Analytical Chemistry, Washington, American Chemical Society, 2014, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.636

Kód RIV

RIV/00216224:14740/14:00073485

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1021/ac402941e

UT WoS

000329548700068

Klíčová slova anglicky

MALDI MS; ICP MS; single run; capillary electrophoresis

Štítky

kontrola MP, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 2. 2018 22:11, prof. Mgr. Jan Preisler, Ph.D.

Anotace

V originále

In this work, a novel approach based on off-line coupling of a single run of capillary electrophoresis (CE) separation to both matrix-assisted laser desorption/ionization (MALDI) and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS) is presented. Using a liquid junction and subatmospheric deposition chamber, CE fractions were extracted from a separation capillary and collected as 20-nL droplets on a custom-built polyethylene terephthalate glycol (PETG) target plate coated with a 10-nm gold layer which guaranteed compatibility with both MALDI and SALD ICP techniques. The MALDI matrix solution was then added to the produced spots. After it was dried, the separation record was consecutively analyzed in MALDI MS and ICP MS instruments. Thus, both proteomic and metallomic information was obtained off-line from a single CE run. The concept was demonstrated by the analysis of a mixture of rabbit liver metallothionein isoforms. In an additional study, the droplets representing the archived separation record were alternately mixed with two different MALDI matrices to obtain complementary information on both the apoproteins and their complexes with metals from a single separation run. The presented technique is a viable alternative to online coupling of column separation to electrospray MS and nebulizer ICP MS.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP206/12/0538, projekt VaV

Název: Nové strategie úpravy vzorků pro desorpční hmotnostní spektrometrii

Investor: Grantová agentura ČR, Nové strategie úpravy vzorků pro desorpční hmotnostní spektrometrii

Citovat

TOMALOVÁ, Iva, Pavla FOLTYNOVÁ, Viktor KANICKÝ a Jan PREISLER. MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics. Analytical Chemistry. Washington: American Chemical Society, 2014, roč. 86, č. 1, s. 647-654. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/ac402941e.

@article{1163964,
   author = {Tomalová, Iva and Foltynová, Pavla and Kanický, Viktor and Preisler, Jan},
   article_location = {Washington},
   article_number = {1},
   doi = {http://dx.doi.org/10.1021/ac402941e},
   keywords = {MALDI MS; ICP MS; single run; capillary electrophoresis},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics},
   url = {http://pubs.acs.org/doi/abs/10.1021/ac402941e},
   volume = {86},
   year = {2014}
}

TY  - JOUR
ID  - 1163964
AU  - Tomalová, Iva - Foltynová, Pavla - Kanický, Viktor - Preisler, Jan
PY  - 2014
TI  - MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics
JF  - Analytical Chemistry
VL  - 86
IS  - 1
SP  - 647-654
EP  - 647-654
PB  - American Chemical Society
SN  - 00032700
KW  - MALDI MS
KW  - ICP MS
KW  - single run
KW  - capillary electrophoresis
UR  - http://pubs.acs.org/doi/abs/10.1021/ac402941e
L2  - http://pubs.acs.org/doi/abs/10.1021/ac402941e
N2  - In this work, a novel approach based on off-line coupling of a single run of capillary electrophoresis (CE) separation to both matrix-assisted laser desorption/ionization (MALDI) and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS) is presented. Using a liquid junction and subatmospheric deposition chamber, CE fractions were extracted from a separation capillary and collected as 20-nL droplets on a custom-built polyethylene terephthalate glycol (PETG) target plate coated with a 10-nm gold layer which guaranteed compatibility with both MALDI and SALD ICP techniques. The MALDI matrix solution was then added to the produced spots. After it was dried, the separation record was consecutively analyzed in MALDI MS and ICP MS instruments. Thus, both proteomic and metallomic information was obtained off-line from a single CE run. The concept was demonstrated by the analysis of a mixture of rabbit liver metallothionein isoforms. In an additional study, the droplets representing the archived separation record were alternately mixed with two different MALDI matrices to obtain complementary information on both the apoproteins and their complexes with metals from a single separation run. The presented technique is a viable alternative to online coupling of column separation to electrospray MS and nebulizer ICP MS.
ER  -

TOMALOVÁ, Iva, Pavla FOLTYNOVÁ, Viktor KANICKÝ a Jan PREISLER. MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics. \textit{Analytical Chemistry}. Washington: American Chemical Society, 2014, roč.~86, č.~1, s.~647-654. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/ac402941e.

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