IMPROVING THE REPEATABILITY OF SAMPLING PROCEDURES FOR EXHALED
BREATH CONDENSATE ANALYSIS

D 2013

IMPROVING THE REPEATABILITY OF SAMPLING PROCEDURES FOR EXHALED BREATH CONDENSATE ANALYSIS

GREGUŠ, Michal; Petr KUBÁŇ and František FORET

Basic information

Original name

IMPROVING THE REPEATABILITY OF SAMPLING PROCEDURES FOR EXHALED BREATH CONDENSATE ANALYSIS

Authors

GREGUŠ, Michal (703 Slovakia, belonging to the institution); Petr KUBÁŇ (203 Czech Republic, guarantor, belonging to the institution) and František FORET (203 Czech Republic, belonging to the institution)

Edition

Vol. 107. Praha, Chemické listy, p. "S370-S372", 3 pp. 2013

Publisher

Česká společnost chemická

Other information

Language

English

Type of outcome

Proceedings paper

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

Publication form

electronic version available online

References:

Chemické listy

RIV identification code

RIV/00216224:14740/13:00066860

Organization unit

Central European Institute of Technology

ISSN

UT WoS

000328730800036

Keywords (in Czech)

kapilární elektroforéza; bezkontaktní konduktometrická detekce; kondenzát vydechovaného vzduch; dávkovací zařízení; standardizace

Keywords in English

capillary electrophoresis; contactless conductivity detection; exhaled breath condensate; sampling device; standardization

Abstract

In the original language

A miniature sampler for exhaled breath condensate (EBC) collection was constructed and the repepatability of the collection procedure was studied. The samples were analyzed by CE-C4D home-made instrument. To improve the repeatability of the EBC collection, first the collection tubes, straws and vials require cleanup to remove unwanted interferents. Second, the breathing pattern should be kept uniform. We demonstrate that by adopting these measures, repeatability can be improved from 21.4– 186.5 % RSD (non-standardized sampling) to 3.6–80.3 % RSD (standardized sampling) and is comparable than with the commercial device (6.6–75.6 % RSD).

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GA13-21919S, research and development project

Name: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.

Investor: Czech Science Foundation

Cite

GREGUŠ, Michal; Petr KUBÁŇ and František FORET. IMPROVING THE REPEATABILITY OF SAMPLING PROCEDURES FOR EXHALED BREATH CONDENSATE ANALYSIS. Online. In František Foret, Jana Křenková, Andras Guttman, Karel Klepárník, Petr Boček. Chemické listy. Vol. 107. Praha: Česká společnost chemická, 2013, p. "S370-S372", 3 pp. ISSN 1213-7103.

@inproceedings{1164066,
   author = {Greguš, Michal and Kubáň, Petr and Foret, František},
   address = {Praha},
   booktitle = {Chemické listy},
   edition = {Vol. 107},
   editor = {František Foret, Jana Křenková, Andras Guttman, Karel Klepárník, Petr Boček},
   keywords = {capillary electrophoresis; contactless conductivity detection; exhaled breath condensate; sampling device; standardization},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Praha},
   pages = {"S370-S372"},
   publisher = {Česká společnost chemická},
   title = {IMPROVING THE REPEATABILITY OF SAMPLING PROCEDURES FOR EXHALED BREATH CONDENSATE ANALYSIS},
   url = {http://www.chemicke-listy.cz/common/content-issue_s3-volume_107-year_2013.html},
   year = {2013}
}

TY  - CONF
ID  - 1164066
AU  - Greguš, Michal - Kubáň, Petr - Foret, František
PY  - 2013
TI  - IMPROVING THE REPEATABILITY OF SAMPLING PROCEDURES FOR EXHALED BREATH CONDENSATE ANALYSIS
PB  - Česká společnost chemická
CY  - Praha
KW  - capillary electrophoresis
KW  - contactless conductivity detection
KW  - exhaled breath condensate
KW  - sampling device
KW  - standardization
UR  - http://www.chemicke-listy.cz/common/content-issue_s3-volume_107-year_2013.html
L2  - http://www.chemicke-listy.cz/common/content-issue_s3-volume_107-year_2013.html
N2  - A miniature sampler for exhaled breath condensate (EBC) collection was constructed and the repepatability of the collection procedure was studied. The samples were analyzed by CE-C4D home-made instrument. To improve the repeatability of the EBC collection, first the collection tubes, straws and vials require cleanup to remove unwanted interferents. Second, the breathing pattern should be kept uniform. We demonstrate that by adopting these measures, repeatability can be improved from 21.4– 186.5 % RSD (non-standardized sampling) to 3.6–80.3 % RSD (standardized sampling) and is comparable than with the commercial device (6.6–75.6 % RSD).
ER  -

GREGUŠ, Michal; Petr KUBÁŇ and František FORET. IMPROVING THE REPEATABILITY OF SAMPLING PROCEDURES FOR EXHALED BREATH CONDENSATE ANALYSIS. Online. In František Foret, Jana Křenková, Andras Guttman, Karel Klepárník, Petr Boček. \textit{Chemické listy}. Vol. 107. Praha: Česká společnost chemická, 2013, p.~''S370-S372'', 3 pp. ISSN~1213-7103.

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