Single-cell-based system to monitor carrier driven cellular
auxin homeostasis

J 2013

Single-cell-based system to monitor carrier driven cellular auxin homeostasis

BARBEZ, Elke; Martina LANKOVA; Marketa PAREZOVA; Alexis MAIZEL; Eva ZAŽÍMALOVÁ et al.

Základní údaje

Originální název

Single-cell-based system to monitor carrier driven cellular auxin homeostasis

Autoři

BARBEZ, Elke; Martina LANKOVA; Marketa PAREZOVA; Alexis MAIZEL; Eva ZAŽÍMALOVÁ; Jan PETRASEK; Jiří FRIML a Jurgen KLEINE-VEHN

Vydání

BMC Plant Biology, London, BioMed Central Ltd. 2013, 1471-2229

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.942

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/13:00072091

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1186/1471-2229-13-20

UT WoS

000315715700001

Klíčová slova anglicky

Auxin homeostasis; DR5; Auxin carrier; Auxin transport

Štítky

ok, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 12. 2019 11:44, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Background: Abundance and distribution of the plant hormone auxin play important roles in plant development. Besides other metabolic processes, various auxin carriers control the cellular level of active auxin and, hence, are major regulators of cellular auxin homeostasis. Despite the developmental importance of auxin transporters, a simple medium-to-high throughput approach to assess carrier activities is still missing. Here we show that carrier driven depletion of cellular auxin correlates with reduced nuclear auxin signaling in tobacco Bright Yellow-2 (BY-2) cell cultures. Results: We developed an easy to use transient single-cell-based system to detect carrier activity. We use the relative changes in signaling output of the auxin responsive promoter element DR5 to indirectly visualize auxin carrier activity. The feasibility of the transient approach was demonstrated by pharmacological and genetic interference with auxin signaling and transport. As a proof of concept, we provide visual evidence that the prominent auxin transport proteins PIN-FORMED (PIN) 2 and PIN5 regulate cellular auxin homeostasis at the plasma membrane and endoplasmic reticulum (ER), respectively. Our data suggest that PIN2 and PIN5 have different sensitivities to the auxin transport inhibitor 1-naphthylphthalamic acid (NPA). Also the putative PIN-LIKES (PILS) auxin carrier activity at the ER is insensitive to NPA in our system, indicating that NPA blocks intercellular, but not intracellular auxin transport. Conclusions: This single-cell-based system is a useful tool by which the activity of putative auxin carriers, such as PINs, PILS and WALLS ARE THIN1 (WAT1), can be indirectly visualized in a medium-to-high throughput manner. Moreover, our single cell system might be useful to investigate also other hormonal signaling pathways, such as cytokinin.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

EE2.3.20.0043, projekt VaV

Název: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů

Přiložené soubory

Barbez_BMC_2013.pdf

Požádat o autorskou verzi souboru

Citovat

BARBEZ, Elke; Martina LANKOVA; Marketa PAREZOVA; Alexis MAIZEL; Eva ZAŽÍMALOVÁ; Jan PETRASEK; Jiří FRIML a Jurgen KLEINE-VEHN. Single-cell-based system to monitor carrier driven cellular auxin homeostasis. BMC Plant Biology. London: BioMed Central Ltd., 2013, roč. 13, February, s. "nestránkováno", 13 s. ISSN 1471-2229. Dostupné z: https://doi.org/10.1186/1471-2229-13-20.

@article{1165394,
   author = {Barbez, Elke and Lankova, Martina and Parezova, Marketa and Maizel, Alexis and Zažímalová, Eva and Petrasek, Jan and Friml, Jiří and KleineandVehn, Jurgen},
   article_location = {London},
   article_number = {February},
   doi = {https://doi.org/10.1186/1471-2229-13-20},
   keywords = {Auxin homeostasis; DR5; Auxin carrier; Auxin transport},
   language = {eng},
   issn = {1471-2229},
   journal = {BMC Plant Biology},
   title = {Single-cell-based system to monitor carrier driven cellular auxin homeostasis},
   url = {http://www.biomedcentral.com/1471-2229/13/20},
   volume = {13},
   year = {2013}
}

TY  - JOUR
ID  - 1165394
AU  - Barbez, Elke - Lankova, Martina - Parezova, Marketa - Maizel, Alexis - Zažímalová, Eva - Petrasek, Jan - Friml, Jiří - Kleine-Vehn, Jurgen
PY  - 2013
TI  - Single-cell-based system to monitor carrier driven cellular auxin homeostasis
JF  - BMC Plant Biology
VL  - 13
IS  - February
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - BioMed Central Ltd.
SN  - 14712229
KW  - Auxin homeostasis
KW  - DR5
KW  - Auxin carrier
KW  - Auxin transport
UR  - http://www.biomedcentral.com/1471-2229/13/20
L2  - http://www.biomedcentral.com/1471-2229/13/20
N2  - Background: Abundance and distribution of the plant hormone auxin play important roles in plant development. Besides other metabolic processes, various auxin carriers control the cellular level of active auxin and, hence, are major regulators of cellular auxin homeostasis. Despite the developmental importance of auxin transporters, a simple medium-to-high throughput approach to assess carrier activities is still missing. Here we show that carrier driven depletion of cellular auxin correlates with reduced nuclear auxin signaling in tobacco Bright Yellow-2 (BY-2) cell cultures. Results: We developed an easy to use transient single-cell-based system to detect carrier activity. We use the relative changes in signaling output of the auxin responsive promoter element DR5 to indirectly visualize auxin carrier activity. The feasibility of the transient approach was demonstrated by pharmacological and genetic interference with auxin signaling and transport. As a proof of concept, we provide visual evidence that the prominent auxin transport proteins PIN-FORMED (PIN) 2 and PIN5 regulate cellular auxin homeostasis at the plasma membrane and endoplasmic reticulum (ER), respectively. Our data suggest that PIN2 and PIN5 have different sensitivities to the auxin transport inhibitor 1-naphthylphthalamic acid (NPA). Also the putative PIN-LIKES (PILS) auxin carrier activity at the ER is insensitive to NPA in our system, indicating that NPA blocks intercellular, but not intracellular auxin transport. Conclusions: This single-cell-based system is a useful tool by which the activity of putative auxin carriers, such as PINs, PILS and WALLS ARE THIN1 (WAT1), can be indirectly visualized in a medium-to-high throughput manner. Moreover, our single cell system might be useful to investigate also other hormonal signaling pathways, such as cytokinin.
ER  -

BARBEZ, Elke; Martina LANKOVA; Marketa PAREZOVA; Alexis MAIZEL; Eva ZAŽÍMALOVÁ; Jan PETRASEK; Jiří FRIML a Jurgen KLEINE-VEHN. Single-cell-based system to monitor carrier driven cellular auxin homeostasis. \textit{BMC Plant Biology}. London: BioMed Central Ltd., 2013, roč.~13, February, s.~''nestránkováno'', 13 s. ISSN~1471-2229. Dostupné z: https://doi.org/10.1186/1471-2229-13-20.

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