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@article{1165521,
author = {MohelnikovaandDuchonova, Beatrice and Brynychova, Veronika and Oliverius, Martin and Honsova, Eva and Kala, Zdeněk and Múčková, Katarína and Soucek, Pavel},
article_location = {USA},
article_number = {4},
doi = {http://dx.doi.org/10.1097/MPA.0b013e318279b861},
keywords = {pancreas; carcinoma; ABC transporters; expression; KRAS},
language = {eng},
issn = {0885-3177},
journal = {Pancreas},
title = {Differences in Transcript Levels of ABC Transporters Between Pancreatic Adenocarcinoma and Nonneoplastic Tissues},
volume = {42},
year = {2013}
}
TY - JOUR
ID - 1165521
AU - Mohelnikova-Duchonova, Beatrice - Brynychova, Veronika - Oliverius, Martin - Honsova, Eva - Kala, Zdeněk - Múčková, Katarína - Soucek, Pavel
PY - 2013
TI - Differences in Transcript Levels of ABC Transporters Between Pancreatic Adenocarcinoma and Nonneoplastic Tissues
JF - Pancreas
VL - 42
IS - 4
SP - 707-716
EP - 707-716
PB - Lippincott Williams Wilkins
SN - 08853177
KW - pancreas
KW - carcinoma
KW - ABC transporters
KW - expression
KW - KRAS
N2 - Objectives: The aim of this study was to evaluate transcript levels of all 49 human ATP-binding cassette transporters (ABCs) in one of the most drug-resistant cancers, namely, the pancreatic ductal adenocarcinoma (PDAC). Association of ABCs levels with clinical-pathologic characteristics and KRAS mutation status was followed as well. Methods: Tumors and adjacent nonneoplastic tissues were obtained from 32 histologically verified PDAC patients. The transcript profile of ABCs was assessed using quantitative real-time polymerase chain reaction with a relative standard curve. KRAS mutations in exon 2 were assessed by high-resolution melting analysis and sequencing. Results: Most ABCs were deregulated in PDAC and 10 ABCs were associated with clinical-pathologic characteristics. KRAS mutations did not change the global expression profile of ABCs. Conclusions: The expression of ABC transporters was significantly deregulated in PDAC tumors when compared to nonmalignant tissues. The observed up-regulation of ABCB4, ABCB11, ABCC1, ABCC3, ABCC5, ABCC10, and ABCG2 in tumors may contribute to the generally poor treatment response of PDAC. The up-regulation of ABCA1, ABCA7, and ABCG1 implicates a serious impairment of cellular cholesterol homeostasis in PDAC. On the other hand, the observed down-regulation of ABCA3, ABCC6, ABCC7, and ABCC8 suggests a possible role of stem cells in the development and progression of PDAC.
ER -
MOHELNIKOVA-DUCHONOVA, Beatrice, Veronika BRYNYCHOVA, Martin OLIVERIUS, Eva HONSOVA, Zdeněk KALA, Katarína MÚČKOVÁ a Pavel SOUCEK. Differences in Transcript Levels of ABC Transporters Between Pancreatic Adenocarcinoma and Nonneoplastic Tissues. \textit{Pancreas}. USA: Lippincott Williams Wilkins, 2013, roč.~42, č.~4, s.~707-716. ISSN~0885-3177. Dostupné z: https://dx.doi.org/10.1097/MPA.0b013e318279b861.
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