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@article{1165521, author = {MohelnikovaandDuchonova, Beatrice and Brynychova, Veronika and Oliverius, Martin and Honsova, Eva and Kala, Zdeněk and Múčková, Katarína and Soucek, Pavel}, article_location = {USA}, article_number = {4}, doi = {http://dx.doi.org/10.1097/MPA.0b013e318279b861}, keywords = {pancreas; carcinoma; ABC transporters; expression; KRAS}, language = {eng}, issn = {0885-3177}, journal = {Pancreas}, title = {Differences in Transcript Levels of ABC Transporters Between Pancreatic Adenocarcinoma and Nonneoplastic Tissues}, volume = {42}, year = {2013} }
TY - JOUR ID - 1165521 AU - Mohelnikova-Duchonova, Beatrice - Brynychova, Veronika - Oliverius, Martin - Honsova, Eva - Kala, Zdeněk - Múčková, Katarína - Soucek, Pavel PY - 2013 TI - Differences in Transcript Levels of ABC Transporters Between Pancreatic Adenocarcinoma and Nonneoplastic Tissues JF - Pancreas VL - 42 IS - 4 SP - 707-716 EP - 707-716 PB - Lippincott Williams Wilkins SN - 08853177 KW - pancreas KW - carcinoma KW - ABC transporters KW - expression KW - KRAS N2 - Objectives: The aim of this study was to evaluate transcript levels of all 49 human ATP-binding cassette transporters (ABCs) in one of the most drug-resistant cancers, namely, the pancreatic ductal adenocarcinoma (PDAC). Association of ABCs levels with clinical-pathologic characteristics and KRAS mutation status was followed as well. Methods: Tumors and adjacent nonneoplastic tissues were obtained from 32 histologically verified PDAC patients. The transcript profile of ABCs was assessed using quantitative real-time polymerase chain reaction with a relative standard curve. KRAS mutations in exon 2 were assessed by high-resolution melting analysis and sequencing. Results: Most ABCs were deregulated in PDAC and 10 ABCs were associated with clinical-pathologic characteristics. KRAS mutations did not change the global expression profile of ABCs. Conclusions: The expression of ABC transporters was significantly deregulated in PDAC tumors when compared to nonmalignant tissues. The observed up-regulation of ABCB4, ABCB11, ABCC1, ABCC3, ABCC5, ABCC10, and ABCG2 in tumors may contribute to the generally poor treatment response of PDAC. The up-regulation of ABCA1, ABCA7, and ABCG1 implicates a serious impairment of cellular cholesterol homeostasis in PDAC. On the other hand, the observed down-regulation of ABCA3, ABCC6, ABCC7, and ABCC8 suggests a possible role of stem cells in the development and progression of PDAC. ER -
MOHELNIKOVA-DUCHONOVA, Beatrice, Veronika BRYNYCHOVA, Martin OLIVERIUS, Eva HONSOVA, Zdeněk KALA, Katarína MÚČKOVÁ a Pavel SOUCEK. Differences in Transcript Levels of ABC Transporters Between Pancreatic Adenocarcinoma and Nonneoplastic Tissues. \textit{Pancreas}. USA: Lippincott Williams Wilkins, 2013, roč.~42, č.~4, s.~707-716. ISSN~0885-3177. Dostupné z: https://dx.doi.org/10.1097/MPA.0b013e318279b861.
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