53BP1 Regulates DSB Repair Using Rif1 to Control 5 ' End
Resection

J 2013

53BP1 Regulates DSB Repair Using Rif1 to Control 5 ' End Resection

ZIMMERMANN, Michal, Francisca LOTTERSBERGER, Sara B. BUONOMO, Agnel SFEIR, Titia DE LANGE et. al.

Základní údaje

Originální název

53BP1 Regulates DSB Repair Using Rif1 to Control 5 ' End Resection

Autoři

ZIMMERMANN, Michal (203 Česká republika, garant, domácí), Francisca LOTTERSBERGER (840 Spojené státy), Sara B. BUONOMO (840 Spojené státy), Agnel SFEIR (840 Spojené státy) a Titia DE LANGE (840 Spojené státy)

Vydání

Science, Washington, American Association for the Advancement of Science, 2013, 0036-8075

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 31.477

Kód RIV

RIV/00216224:14740/13:00066922

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1126/science.1231573

UT WoS

000314585600044

Klíčová slova anglicky

PROTEIN 53BP1; DNA; RECOMBINATION; TELOMERES; DOMAINS; CELLS; CTIP

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 4. 2014 00:28, Olga Křížová

Anotace

V originále

The choice between double-strand break (DSB) repair by either homology-directed repair (HDR) or nonhomologous end joining (NHEJ) is tightly regulated. Defects in this regulation can induce genome instability and cancer. 53BP1 is critical for the control of DSB repair, promoting NHEJ, and inhibiting the 5' end resection needed for HDR. Using dysfunctional telomeres and genome-wide DSBs, we identify Rif1 as the main factor used by 53BP1 to impair 5' end resection. Rif1 inhibits resection involving CtIP, BLM, and Exo1; limits accumulation of BRCA1/BARD1 complexes at sites of DNA damage; and defines one of the mechanisms by which 53BP1 causes chromosomal abnormalities in Brca1-deficient cells. These data establish Rif1 as an important contributor to the control of DSB repair by 53BP1.

Návaznosti

GAP205/12/0550, projekt VaV

Název: Dynamika vzniku shelterinového komplexu

Investor: Grantová agentura ČR, Dynamika vzniku shelterinového komplexu

Citovat

ZIMMERMANN, Michal, Francisca LOTTERSBERGER, Sara B. BUONOMO, Agnel SFEIR a Titia DE LANGE. 53BP1 Regulates DSB Repair Using Rif1 to Control 5 ' End Resection. Science. Washington: American Association for the Advancement of Science, 2013, roč. 339, č. 6120, s. 700-704. ISSN 0036-8075. Dostupné z: https://dx.doi.org/10.1126/science.1231573.

@article{1166233,
   author = {Zimmermann, Michal and Lottersberger, Francisca and Buonomo, Sara B. and Sfeir, Agnel and de Lange, Titia},
   article_location = {Washington},
   article_number = {6120},
   doi = {http://dx.doi.org/10.1126/science.1231573},
   keywords = {PROTEIN 53BP1; DNA; RECOMBINATION; TELOMERES; DOMAINS; CELLS; CTIP},
   language = {eng},
   issn = {0036-8075},
   journal = {Science},
   title = {53BP1 Regulates DSB Repair Using Rif1 to Control 5 ' End Resection},
   volume = {339},
   year = {2013}
}

TY  - JOUR
ID  - 1166233
AU  - Zimmermann, Michal - Lottersberger, Francisca - Buonomo, Sara B. - Sfeir, Agnel - de Lange, Titia
PY  - 2013
TI  - 53BP1 Regulates DSB Repair Using Rif1 to Control 5 ' End Resection
JF  - Science
VL  - 339
IS  - 6120
SP  - 700-704
EP  - 700-704
PB  - American Association for the Advancement of Science
SN  - 00368075
KW  - PROTEIN 53BP1
KW  - DNA
KW  - RECOMBINATION
KW  - TELOMERES
KW  - DOMAINS
KW  - CELLS
KW  - CTIP
N2  - The choice between double-strand break (DSB) repair by either homology-directed repair (HDR) or nonhomologous end joining (NHEJ) is tightly regulated. Defects in this regulation can induce genome instability and cancer. 53BP1 is critical for the control of DSB repair, promoting NHEJ, and inhibiting the 5' end resection needed for HDR. Using dysfunctional telomeres and genome-wide DSBs, we identify Rif1 as the main factor used by 53BP1 to impair 5' end resection. Rif1 inhibits resection involving CtIP, BLM, and Exo1; limits accumulation of BRCA1/BARD1 complexes at sites of DNA damage; and defines one of the mechanisms by which 53BP1 causes chromosomal abnormalities in Brca1-deficient cells. These data establish Rif1 as an important contributor to the control of DSB repair by 53BP1.
ER  -

ZIMMERMANN, Michal, Francisca LOTTERSBERGER, Sara B. BUONOMO, Agnel SFEIR a Titia DE LANGE. 53BP1 Regulates DSB Repair Using Rif1 to Control 5 ' End Resection. \textit{Science}. Washington: American Association for the Advancement of Science, 2013, roč.~339, č.~6120, s.~700-704. ISSN~0036-8075. Dostupné z: https://dx.doi.org/10.1126/science.1231573.

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