Novel metabolites in cyanobacterium Cylindrospermopsis
raciborskii with potencies to inhibit gap junctional
intercellular communication

J 2013

Novel metabolites in cyanobacterium Cylindrospermopsis raciborskii with potencies to inhibit gap junctional intercellular communication

NOVÁKOVÁ, Kateřina, Jiří KOHOUTEK, Ondřej ADAMOVSKÝ, W. BRACK, M. KRAUSS et. al.

Základní údaje

Originální název

Novel metabolites in cyanobacterium Cylindrospermopsis raciborskii with potencies to inhibit gap junctional intercellular communication

Autoři

NOVÁKOVÁ, Kateřina (203 Česká republika, domácí), Jiří KOHOUTEK (203 Česká republika, domácí), Ondřej ADAMOVSKÝ (203 Česká republika, domácí), W. BRACK (276 Německo), M. KRAUSS (276 Německo) a Luděk BLÁHA (203 Česká republika, garant, domácí)

Vydání

JOURNAL OF HAZARDOUS MATERIALS, AMSTERDAM, ELSEVIER SCIENCE BV, 2013, 0304-3894

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.331

Kód RIV

RIV/00216224:14310/13:00066936

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.jhazmat.2013.09.007

UT WoS

000329595500068

Klíčová slova anglicky

Cyanobacteria; Exudate; Tumor promotion; Fractionation

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 4. 2014 13:58, Ing. Andrea Mikešková

Anotace

V originále

Despite intensive research into toxic bloom-forming cyanobacteria, the majority of their metabolites remain unknown. The present study explored in detail a novel bioactivity identified in cyanobacteria, i.e. inhibition of gap junctional intercellular communication (GJIC), a marker of tumor promotion. The extracellular mixture (exudate) of the cyanobacterial strain Cylindrospermopsis raciborskii (SAG 1.97) was fractionated by semi-preparative reversed phase HPLC, and the fractions assessed for their potencies to inhibit GJIC. Two non-polar fractions that significantly inhibited GJIC were further fractionated, tested and analyzed using multiple mass spectrometric methods. Investigations led to the identification of a putative chemical compound (molecular formula C18H34O3, m/z 299.2581 for the [M+H](+) ion) responsible for observed bioactivities. Specific inhibitors of signaling pathways were used to screen for biochemical mechanisms beyond GJIC inhibition, and the results indicate the involvement of ERK1/2 kinases via a mechanism related to the action of epidermal growth factor EGF but clearly distinct from other anthropogenic tumor promoters like polychlorinated biphenyls or polycyclic aromatic hydrocarbons. The chemical and in vitro toxicological characterizations of the newly described metabolite provide important insights into the still poorly understood health impacts of complex toxic cyanobacterial blooms and indicate that currently applied monitoring practices may underestimate actual risks.

Návaznosti

ED0001/01/01, projekt VaV

Název: CETOCOEN

GAP503/12/0553, projekt VaV

Název: Metabolity sinic jako potenciální endokrinní disruptory

Investor: Grantová agentura ČR, Metabolity sinic jako potenciální endokrinní disruptory

GA524/08/0496, projekt VaV

Název: Mechanismy nádorové promoce metabolitů toxických sinic

Investor: Grantová agentura ČR, Mechanismy nádorové promoce metabolitů toxických sinic

Citovat

NOVÁKOVÁ, Kateřina, Jiří KOHOUTEK, Ondřej ADAMOVSKÝ, W. BRACK, M. KRAUSS a Luděk BLÁHA. Novel metabolites in cyanobacterium Cylindrospermopsis raciborskii with potencies to inhibit gap junctional intercellular communication. JOURNAL OF HAZARDOUS MATERIALS. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, roč. 262, č. 2013, s. 571-579. ISSN 0304-3894. Dostupné z: https://dx.doi.org/10.1016/j.jhazmat.2013.09.007.

@article{1166993,
   author = {Nováková, Kateřina and Kohoutek, Jiří and Adamovský, Ondřej and Brack, W. and Krauss, M. and Bláha, Luděk},
   article_location = {AMSTERDAM},
   article_number = {2013},
   doi = {http://dx.doi.org/10.1016/j.jhazmat.2013.09.007},
   keywords = {Cyanobacteria; Exudate; Tumor promotion; Fractionation},
   language = {eng},
   issn = {0304-3894},
   journal = {JOURNAL OF HAZARDOUS MATERIALS},
   title = {Novel metabolites in cyanobacterium Cylindrospermopsis raciborskii with potencies to inhibit gap junctional intercellular communication},
   volume = {262},
   year = {2013}
}

TY  - JOUR
ID  - 1166993
AU  - Nováková, Kateřina - Kohoutek, Jiří - Adamovský, Ondřej - Brack, W. - Krauss, M. - Bláha, Luděk
PY  - 2013
TI  - Novel metabolites in cyanobacterium Cylindrospermopsis raciborskii with potencies to inhibit gap junctional intercellular communication
JF  - JOURNAL OF HAZARDOUS MATERIALS
VL  - 262
IS  - 2013
SP  - 571-579
EP  - 571-579
PB  - ELSEVIER SCIENCE BV
SN  - 03043894
KW  - Cyanobacteria
KW  - Exudate
KW  - Tumor promotion
KW  - Fractionation
N2  - Despite intensive research into toxic bloom-forming cyanobacteria, the majority of their metabolites remain unknown. The present study explored in detail a novel bioactivity identified in cyanobacteria, i.e. inhibition of gap junctional intercellular communication (GJIC), a marker of tumor promotion. The extracellular mixture (exudate) of the cyanobacterial strain Cylindrospermopsis raciborskii (SAG 1.97) was fractionated by semi-preparative reversed phase HPLC, and the fractions assessed for their potencies to inhibit GJIC. Two non-polar fractions that significantly inhibited GJIC were further fractionated, tested and analyzed using multiple mass spectrometric methods. Investigations led to the identification of a putative chemical compound (molecular formula C18H34O3, m/z 299.2581 for the [M+H](+) ion) responsible for observed bioactivities. Specific inhibitors of signaling pathways were used to screen for biochemical mechanisms beyond GJIC inhibition, and the results indicate the involvement of ERK1/2 kinases via a mechanism related to the action of epidermal growth factor EGF but clearly distinct from other anthropogenic tumor promoters like polychlorinated biphenyls or polycyclic aromatic hydrocarbons. The chemical and in vitro toxicological characterizations of the newly described metabolite provide important insights into the still poorly understood health impacts of complex toxic cyanobacterial blooms and indicate that currently applied monitoring practices may underestimate actual risks.
ER  -

NOVÁKOVÁ, Kateřina, Jiří KOHOUTEK, Ondřej ADAMOVSKÝ, W. BRACK, M. KRAUSS a Luděk BLÁHA. Novel metabolites in cyanobacterium Cylindrospermopsis raciborskii with potencies to inhibit gap junctional intercellular communication. \textit{JOURNAL OF HAZARDOUS MATERIALS}. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, roč.~262, č.~2013, s.~571-579. ISSN~0304-3894. Dostupné z: https://dx.doi.org/10.1016/j.jhazmat.2013.09.007.

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