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@article{1166993,
author = {Nováková, Kateřina and Kohoutek, Jiří and Adamovský, Ondřej and Brack, W. and Krauss, M. and Bláha, Luděk},
article_location = {AMSTERDAM},
article_number = {2013},
doi = {http://dx.doi.org/10.1016/j.jhazmat.2013.09.007},
keywords = {Cyanobacteria; Exudate; Tumor promotion; Fractionation},
language = {eng},
issn = {0304-3894},
journal = {JOURNAL OF HAZARDOUS MATERIALS},
title = {Novel metabolites in cyanobacterium Cylindrospermopsis raciborskii with potencies to inhibit gap junctional intercellular communication},
volume = {262},
year = {2013}
}
TY - JOUR
ID - 1166993
AU - Nováková, Kateřina - Kohoutek, Jiří - Adamovský, Ondřej - Brack, W. - Krauss, M. - Bláha, Luděk
PY - 2013
TI - Novel metabolites in cyanobacterium Cylindrospermopsis raciborskii with potencies to inhibit gap junctional intercellular communication
JF - JOURNAL OF HAZARDOUS MATERIALS
VL - 262
IS - 2013
SP - 571-579
EP - 571-579
PB - ELSEVIER SCIENCE BV
SN - 03043894
KW - Cyanobacteria
KW - Exudate
KW - Tumor promotion
KW - Fractionation
N2 - Despite intensive research into toxic bloom-forming cyanobacteria, the majority of their metabolites remain unknown. The present study explored in detail a novel bioactivity identified in cyanobacteria, i.e. inhibition of gap junctional intercellular communication (GJIC), a marker of tumor promotion. The extracellular mixture (exudate) of the cyanobacterial strain Cylindrospermopsis raciborskii (SAG 1.97) was fractionated by semi-preparative reversed phase HPLC, and the fractions assessed for their potencies to inhibit GJIC. Two non-polar fractions that significantly inhibited GJIC were further fractionated, tested and analyzed using multiple mass spectrometric methods. Investigations led to the identification of a putative chemical compound (molecular formula C18H34O3, m/z 299.2581 for the [M+H](+) ion) responsible for observed bioactivities. Specific inhibitors of signaling pathways were used to screen for biochemical mechanisms beyond GJIC inhibition, and the results indicate the involvement of ERK1/2 kinases via a mechanism related to the action of epidermal growth factor EGF but clearly distinct from other anthropogenic tumor promoters like polychlorinated biphenyls or polycyclic aromatic hydrocarbons. The chemical and in vitro toxicological characterizations of the newly described metabolite provide important insights into the still poorly understood health impacts of complex toxic cyanobacterial blooms and indicate that currently applied monitoring practices may underestimate actual risks.
ER -
NOVÁKOVÁ, Kateřina, Jiří KOHOUTEK, Ondřej ADAMOVSKÝ, W. BRACK, M. KRAUSS a Luděk BLÁHA. Novel metabolites in cyanobacterium Cylindrospermopsis raciborskii with potencies to inhibit gap junctional intercellular communication. \textit{JOURNAL OF HAZARDOUS MATERIALS}. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, roč.~262, č.~2013, s.~571-579. ISSN~0304-3894. Dostupné z: https://dx.doi.org/10.1016/j.jhazmat.2013.09.007.
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