BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN
auxin transporters and auxin-mediated development in
Arabidopsis

J 2014

BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in Arabidopsis

TANAKA, Hirokazu, Tomasz NODZYNSKI, Saeko KITAKURA, Mugurel I. FERARU, Michiko SASABE et. al.

Základní údaje

Originální název

BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in Arabidopsis

Autoři

TANAKA, Hirokazu (392 Japonsko, garant), Tomasz NODZYNSKI (616 Polsko, domácí), Saeko KITAKURA (392 Japonsko), Mugurel I. FERARU (40 Rakousko), Michiko SASABE (392 Japonsko), Tomoni ISHIKAWA (392 Japonsko), Jürgen KLEINE-VEHN (40 Rakousko), Tatsuo KAKIMOTO (392 Japonsko) a Jiří FRIML (203 Česká republika, domácí)

Vydání

PLANT AND CELL PHYSIOLOGY, Oxford, Oxford University Press, 2014, 0032-0781

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.931

Kód RIV

RIV/00216224:14740/14:00073516

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1093/pcp/pct196

UT WoS

000334679500008

Klíčová slova anglicky

Arabidopsis thaliana; Auxin; Embryogenesis; Exocytosis; PIN-FORMED

Štítky

kontrola MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2015 11:03, Martina Prášilová

Anotace

V originále

Correct positioning of membrane proteins is an essential process in eukaryotic organisms. The plant hormone auxin is distributed through intercellular transport and triggers various cellular responses. Auxin transporters of the PIN-FORMED (PIN) family localize asymmetrically at the plasma membrane (PM) and mediate the directional transport of auxin between cells. A fungal toxin, brefeldin A (BFA), inhibits a subset of guanine nucleotide exchange factors for ADP-ribosylation factor small GTPases (ARF GEFs) including GNOM, which plays a major role in localization of PIN1 predominantly to the basal side of the PM. The Arabidopsis genome encodes 19 ARF-related putative GTPases. However, ARF components involved in PIN1 localization have been genetically poorly defined. Using a fluorescence imaging-based forward genetic approach, we identified an Arabidopsis mutant, bfa-visualized exocytic trafficking defective1 (bex1), in which PM localization of PIN1-green fluorescent protein (GFP) as well as development is hypersensitive to BFA. We found that in bex1 a member of the ARF1 gene family, ARF1A1C, was mutated. ARF1A1C localizes to the trans-Golgi network/early endosome and Golgi apparatus, acts synergistically to BEN1/MIN7 ARF GEF and is important for PIN recycling to the PM. Consistent with the developmental importance of PIN proteins, functional interference with ARF1 resulted in an impaired auxin response gradient and various developmental defects including embryonic patterning defects and growth arrest. Our results show that ARF1A1C is essential for recycling of PIN auxin transporters and for various auxin-dependent developmental processes.

Návaznosti

EE2.3.20.0043, projekt VaV

Název: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GA13-40637S, projekt VaV

Název: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Grantová agentura ČR, Genetické studie k identifikaci molekulárních mechanizmu bunecné polarity a auxinového transportu v rostlinách

Citovat

TANAKA, Hirokazu, Tomasz NODZYNSKI, Saeko KITAKURA, Mugurel I. FERARU, Michiko SASABE, Tomoni ISHIKAWA, Jürgen KLEINE-VEHN, Tatsuo KAKIMOTO a Jiří FRIML. BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in Arabidopsis. PLANT AND CELL PHYSIOLOGY. Oxford: Oxford University Press, 2014, roč. 55, č. 4, s. 737-749. ISSN 0032-0781. Dostupné z: https://dx.doi.org/10.1093/pcp/pct196.

@article{1168119,
   author = {Tanaka, Hirokazu and Nodzynski, Tomasz and Kitakura, Saeko and Feraru, Mugurel I. and Sasabe, Michiko and Ishikawa, Tomoni and KleineandVehn, Jürgen and Kakimoto, Tatsuo and Friml, Jiří},
   article_location = {Oxford},
   article_number = {4},
   doi = {http://dx.doi.org/10.1093/pcp/pct196},
   keywords = {Arabidopsis thaliana; Auxin; Embryogenesis; Exocytosis; PIN-FORMED},
   language = {eng},
   issn = {0032-0781},
   journal = {PLANT AND CELL PHYSIOLOGY},
   title = {BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in Arabidopsis},
   url = {http://pcp.oxfordjournals.org/content/early/2014/01/27/pcp.pct196.full},
   volume = {55},
   year = {2014}
}

TY  - JOUR
ID  - 1168119
AU  - Tanaka, Hirokazu - Nodzynski, Tomasz - Kitakura, Saeko - Feraru, Mugurel I. - Sasabe, Michiko - Ishikawa, Tomoni - Kleine-Vehn, Jürgen - Kakimoto, Tatsuo - Friml, Jiří
PY  - 2014
TI  - BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in Arabidopsis
JF  - PLANT AND CELL PHYSIOLOGY
VL  - 55
IS  - 4
SP  - 737-749
EP  - 737-749
PB  - Oxford University Press
SN  - 00320781
KW  - Arabidopsis thaliana
KW  - Auxin
KW  - Embryogenesis
KW  - Exocytosis
KW  - PIN-FORMED
UR  - http://pcp.oxfordjournals.org/content/early/2014/01/27/pcp.pct196.full
L2  - http://pcp.oxfordjournals.org/content/early/2014/01/27/pcp.pct196.full
N2  - Correct positioning of membrane proteins is an essential process in eukaryotic organisms. The plant hormone auxin is distributed through intercellular transport and triggers various cellular responses. Auxin transporters of the PIN-FORMED (PIN) family localize asymmetrically at the plasma membrane (PM) and mediate the directional transport of auxin between cells. A fungal toxin, brefeldin A (BFA), inhibits a subset of guanine nucleotide exchange factors for ADP-ribosylation factor small GTPases (ARF GEFs) including GNOM, which plays a major role in localization of PIN1 predominantly to the basal side of the PM. The Arabidopsis genome encodes 19 ARF-related putative GTPases. However, ARF components involved in PIN1 localization have been genetically poorly defined. Using a fluorescence imaging-based forward genetic approach, we identified an Arabidopsis mutant, bfa-visualized exocytic trafficking defective1 (bex1), in which PM localization of PIN1-green fluorescent protein (GFP) as well as development is hypersensitive to BFA. We found that in bex1 a member of the ARF1 gene family, ARF1A1C, was mutated. ARF1A1C localizes to the trans-Golgi network/early endosome and Golgi apparatus, acts synergistically to BEN1/MIN7 ARF GEF and is important for PIN recycling to the PM. Consistent with the developmental importance of PIN proteins, functional interference with ARF1 resulted in an impaired auxin response gradient and various developmental defects including embryonic patterning defects and growth arrest. Our results show that ARF1A1C is essential for recycling of PIN auxin transporters and for various auxin-dependent developmental processes.
ER  -

TANAKA, Hirokazu, Tomasz NODZYNSKI, Saeko KITAKURA, Mugurel I. FERARU, Michiko SASABE, Tomoni ISHIKAWA, Jürgen KLEINE-VEHN, Tatsuo KAKIMOTO a Jiří FRIML. BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in Arabidopsis. \textit{PLANT AND CELL PHYSIOLOGY}. Oxford: Oxford University Press, 2014, roč.~55, č.~4, s.~737-749. ISSN~0032-0781. Dostupné z: https://dx.doi.org/10.1093/pcp/pct196.

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