Classification of Aeromonas spp. strains from human clinical
material

a 2013

Classification of Aeromonas spp. strains from human clinical material

SEDLÁČEK, Ivo, Eva KROUPOVÁ, Michaela SEDLÁČKOVÁ, Eva KREJČÍ, Pavel ŠVEC et. al.

Základní údaje

Originální název

Classification of Aeromonas spp. strains from human clinical material

Autoři

SEDLÁČEK, Ivo (203 Česká republika, garant, domácí), Eva KROUPOVÁ (203 Česká republika, domácí), Michaela SEDLÁČKOVÁ (203 Česká republika), Eva KREJČÍ (203 Česká republika) a Pavel ŠVEC (203 Česká republika, domácí)

Vydání

XXXII Annual Meeting of the European Culture Collections' Organization (ECCO XXXI), 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/13:00072519

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Aeromonas; taxonomy; identification; human clinical material

Změněno: 29. 4. 2014 10:54, Ing. Andrea Mikešková

Anotace

V originále

Aeromonads are ubiquitous in aquatic environments and several species have been recognized as pathogens to a variety of animals including humans. The genus Aeromonas includes a tight group of species and subspecies, some of which have required reclassification. The present study was aimed on aeromonads isolated from human clinical material and classified by biotyping and ribotyping techniques. A group of 75 human aeromonads was identified on the complex level by biotyping as Aeromonas hydrophila complex and/or Aeromonas sobria complex. Identification to the species level was not reliable by biotyping and without additional techniques biotyping cannot be used to delineate closely related species of Aeromonas. Ribotyping clearly distinguished both complexes on the base of fragment lengths and furthermore in several cases enabled correct species identification. Aeromonas hydrophila complex yielded only long fragments in range 2 kbp to 20 kbp, while Aeromonas sobria complex showed also smaller fragments below 2 kbp. Aeromonas hydrophila subsp. hydrophila was predominant species in A. hydrophila complex and we did not prove the recently mentioned position of Aeromonas aquariorum as widely distributed species in clinical specimens and previously misidentified as A. hydrophila. Among A. sobria complex isolates the Aeromonas veronii bv. sobria strains were very frequent. Both taxa, A. hydrophila subsp. hydrophila and A. veronii bv. sobria showed great heterogeneity of ribotyping patterns in several clusters. Ribotyping results also proved occurrence of rare pathogens Aeromonas hydrophila subsp. dhakensis, Aeromonas bestiarum, Aeromonas jandaei, Aeromonas veronii bv. veronii and Aeromonas allosaccharophila in diarrheic stool samples. Species variability of aeromonads from clinical samples was not high and ribotyping was appeared as useful tool for reliable strain typing and species identification of aeromonads in combination with biotyping.

Návaznosti

EE2.3.20.0183, projekt VaV

Název: Centrum experimentální biomedicíny

Citovat

SEDLÁČEK, Ivo, Eva KROUPOVÁ, Michaela SEDLÁČKOVÁ, Eva KREJČÍ a Pavel ŠVEC. Classification of Aeromonas spp. strains from human clinical material. In XXXII Annual Meeting of the European Culture Collections' Organization (ECCO XXXI). 2013.

@proceedings{1168483,
   author = {Sedláček, Ivo and Kroupová, Eva and Sedláčková, Michaela and Krejčí, Eva and Švec, Pavel},
   booktitle = {XXXII Annual Meeting of the European Culture Collections' Organization (ECCO XXXI)},
   keywords = {Aeromonas; taxonomy; identification; human clinical material},
   language = {eng},
   title = {Classification of Aeromonas spp. strains from human clinical material},
   year = {2013}
}

TY  - CONF
ID  - 1168483
AU  - Sedláček, Ivo - Kroupová, Eva - Sedláčková, Michaela - Krejčí, Eva - Švec, Pavel
PY  - 2013
TI  - Classification of Aeromonas spp. strains from human clinical material
KW  - Aeromonas
KW  - taxonomy
KW  - identification
KW  - human clinical material
N2  - Aeromonads are ubiquitous in aquatic environments and several species have been recognized as pathogens to a variety of animals including humans. The genus Aeromonas includes a tight group of species and subspecies, some of which have required reclassification. The present study was aimed on aeromonads isolated from human clinical material and classified by biotyping and ribotyping techniques. A group of 75 human aeromonads was identified on the complex level by biotyping as Aeromonas hydrophila complex and/or Aeromonas sobria complex. Identification to the species level was not reliable by biotyping and without additional techniques biotyping cannot be used to delineate closely related species of Aeromonas. Ribotyping clearly distinguished both complexes on the base of fragment lengths and furthermore in several cases enabled correct species identification. Aeromonas hydrophila complex yielded only long fragments in range 2 kbp to 20 kbp, while Aeromonas sobria complex showed also smaller fragments below 2 kbp. Aeromonas hydrophila subsp. hydrophila was predominant species in A. hydrophila complex and we did not prove the recently mentioned position of Aeromonas aquariorum as widely distributed species in clinical specimens and previously misidentified as A. hydrophila. Among A. sobria complex isolates the Aeromonas veronii bv. sobria strains were very frequent. Both taxa, A. hydrophila subsp. hydrophila and A. veronii bv. sobria showed great heterogeneity of ribotyping patterns in several clusters. Ribotyping results also proved occurrence of rare pathogens Aeromonas hydrophila subsp. dhakensis, Aeromonas bestiarum, Aeromonas jandaei, Aeromonas veronii bv. veronii and Aeromonas allosaccharophila in diarrheic stool samples. Species variability of aeromonads from clinical samples was not high and ribotyping was appeared as useful tool for reliable strain typing and species identification of aeromonads in combination with biotyping.
ER  -

SEDLÁČEK, Ivo, Eva KROUPOVÁ, Michaela SEDLÁČKOVÁ, Eva KREJČÍ a Pavel ŠVEC. Classification of Aeromonas spp. strains from human clinical material. In \textit{XXXII Annual Meeting of the European Culture Collections' Organization (ECCO XXXI)}. 2013.

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