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@proceedings{1168560,
author = {Kolářová, Lenka and Vaňhara, Petr and Puig Romero, Mireia and Salvado, Victoria and Hampl, Aleš and Havel, Josef},
booktitle = {European Symposium on Atomic Spectrometry ESAS 2014 & 15th Czech -Slovak Spectroscopic Conference, No. TP37},
keywords = {MALDI MS; Nanotechnology; Nanoparticles; Nanoflowers; Tissue profiling},
language = {eng},
isbn = {978-80-905704-1-2},
title = {Use of nanomaterials for TOF mass spectrometry of complex biomolecules and for tissue profiling},
year = {2014}
}
TY - CONF
ID - 1168560
AU - Kolářová, Lenka - Vaňhara, Petr - Puig Romero, Mireia - Salvado, Victoria - Hampl, Aleš - Havel, Josef
PY - 2014
TI - Use of nanomaterials for TOF mass spectrometry of complex biomolecules and for tissue profiling
SN - 9788090570412
KW - MALDI MS
KW - Nanotechnology
KW - Nanoparticles
KW - Nanoflowers
KW - Tissue profiling
N2 - Mass spectrometry (MS) of complex biomolecules is mostly performed using Matrix-Assisted Laser Desorption Ionization (MALDI) with Time of Flight (TOF) detection. Development of nanotechnology and nanomaterials (NMs) and nanoparticles (NPs) with their unique physical and chemical properties brought to MS alternative media for efficient ionization of analytes and accelerated development of Surface-Assisted Laser Desorption Ionization (SALDI), Surface-Enhanced Laser Desorption Ionization (SELDI) and Nano-Assisted Laser Desorption Ionization (NALDI). These techniques contributed significantly to MS [1]. NMs possess also extensive applications in MALDI TOF MS tissue imaging in current nanomedicine [2]. In this work, we studied applications of various NMs (either as matrices or surfaces) in MS analysis of histological tissue sections. We also evaluated effect of NMs on the ionization of various peptides and the comparison with common organic matrices was done. LDI of biomolecules from different metallic and/or nano-surfaces documented that NPs increase the ionization, improve homogeneity of samples and reduce the background of salts as compared with conventional matrices. Our data documented that NMs increase signal intensity in mass spectra of various peptides but also of very complex tissue samples. NMs supported MALDI-TOF MS thus represent efficient technique advancing global approaches, such as tissue proteomics.
ER -
KOLÁŘOVÁ, Lenka, Petr VAŇHARA, Mireia PUIG ROMERO, Victoria SALVADO, Aleš HAMPL a Josef HAVEL. Use of nanomaterials for TOF mass spectrometry of complex biomolecules and for tissue profiling. In \textit{European Symposium on Atomic Spectrometry ESAS 2014 \&{} 15th Czech -Slovak Spectroscopic Conference, No. TP37}. 2014. ISBN~978-80-905704-1-2.
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