HAENSEL, Robert, Frank LOEHR, Lukáš TRANTÍREK a Volker DOETSCH. High-Resolution Insight into G-Overhang Architecture. Journal of the American Chemical Society. Washington: American Chemical Society, 2013, roč. 135, č. 7, s. 2816-2824. ISSN 0002-7863. Dostupné z: https://dx.doi.org/10.1021/ja312403b. |
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@article{1169515, author = {Haensel, Robert and Loehr, Frank and Trantírek, Lukáš and Doetsch, Volker}, article_location = {Washington}, article_number = {7}, doi = {http://dx.doi.org/10.1021/ja312403b}, keywords = {HUMAN TELOMERIC DNA; INTRAMOLECULAR G-QUADRUPLEX; K+ SOLUTION; NMR-SPECTROSCOPY; SMALL-MOLECULE; HUMAN CANCER; PULSED EPR; STABILITY; SEQUENCE; CONFORMATIONS}, language = {eng}, issn = {0002-7863}, journal = {Journal of the American Chemical Society}, title = {High-Resolution Insight into G-Overhang Architecture}, volume = {135}, year = {2013} }
TY - JOUR ID - 1169515 AU - Haensel, Robert - Loehr, Frank - Trantírek, Lukáš - Doetsch, Volker PY - 2013 TI - High-Resolution Insight into G-Overhang Architecture JF - Journal of the American Chemical Society VL - 135 IS - 7 SP - 2816-2824 EP - 2816-2824 PB - American Chemical Society SN - 00027863 KW - HUMAN TELOMERIC DNA KW - INTRAMOLECULAR G-QUADRUPLEX KW - K+ SOLUTION KW - NMR-SPECTROSCOPY KW - SMALL-MOLECULE KW - HUMAN CANCER KW - PULSED EPR KW - STABILITY KW - SEQUENCE KW - CONFORMATIONS N2 - NMR and fluorescence spectroscopy were used to address the effect of intracellular molecular crowding and related hydration on a model telomeric G-quadruplex (G4) DNA structure (d(AG(3)(TTAGGG)(3))). d(AG(3)(TTAGGG)(3)) prevalently adopted the hybrid-1 conformation in vivo, ex vivo, and in dilute potassium-based solution, while it formed the parallel propeller fold in water-depleted potassium-based solution, a commonly used model system for studying intracellular molecular crowding. The dilute potassium-based solution appeared to imitate the properties of the cellular environment required for d(AG(3)(TTAGGG)(3)) folding under in vivo and ex vivo conditions. High-resolution NMR investigations of site-specifically N-15-labeled G4 units in native-like single-stranded telomeric DNA revealed that the 3'-terminal and internal G4 unit predominantly coexist in 2-tetrad antiparallel basket and hybrid-2 structures that are arranged in "beads-on-a-string"-like fashion. Our data provide the first high-resolution insight into the telomeric G-overhang architecture under essentially physiological conditions and identify the 2-tetrad antiparallel basket and hybrid-2 topologies as the structural targets for the development of telomere-specific G4 ligands. ER -
HAENSEL, Robert, Frank LOEHR, Lukáš TRANTÍREK a Volker DOETSCH. High-Resolution Insight into G-Overhang Architecture. \textit{Journal of the American Chemical Society}. Washington: American Chemical Society, 2013, roč.~135, č.~7, s.~2816-2824. ISSN~0002-7863. Dostupné z: https://dx.doi.org/10.1021/ja312403b.
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