High-Resolution Insight into G-Overhang Architecture

J 2013

High-Resolution Insight into G-Overhang Architecture

HAENSEL, Robert, Frank LOEHR, Lukáš TRANTÍREK a Volker DOETSCH

Základní údaje

Originální název

High-Resolution Insight into G-Overhang Architecture

Autoři

HAENSEL, Robert (276 Německo), Frank LOEHR (276 Německo), Lukáš TRANTÍREK (203 Česká republika, garant, domácí) a Volker DOETSCH (276 Německo)

Vydání

Journal of the American Chemical Society, Washington, American Chemical Society, 2013, 0002-7863

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 11.444

Kód RIV

RIV/00216224:14740/13:00072647

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1021/ja312403b

UT WoS

000315373000062

Klíčová slova anglicky

HUMAN TELOMERIC DNA; INTRAMOLECULAR G-QUADRUPLEX; K+ SOLUTION; NMR-SPECTROSCOPY; SMALL-MOLECULE; HUMAN CANCER; PULSED EPR; STABILITY; SEQUENCE; CONFORMATIONS

Štítky

ok, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2014 08:25, Olga Křížová

Anotace

V originále

NMR and fluorescence spectroscopy were used to address the effect of intracellular molecular crowding and related hydration on a model telomeric G-quadruplex (G4) DNA structure (d(AG(3)(TTAGGG)(3))). d(AG(3)(TTAGGG)(3)) prevalently adopted the hybrid-1 conformation in vivo, ex vivo, and in dilute potassium-based solution, while it formed the parallel propeller fold in water-depleted potassium-based solution, a commonly used model system for studying intracellular molecular crowding. The dilute potassium-based solution appeared to imitate the properties of the cellular environment required for d(AG(3)(TTAGGG)(3)) folding under in vivo and ex vivo conditions. High-resolution NMR investigations of site-specifically N-15-labeled G4 units in native-like single-stranded telomeric DNA revealed that the 3'-terminal and internal G4 unit predominantly coexist in 2-tetrad antiparallel basket and hybrid-2 structures that are arranged in "beads-on-a-string"-like fashion. Our data provide the first high-resolution insight into the telomeric G-overhang architecture under essentially physiological conditions and identify the 2-tetrad antiparallel basket and hybrid-2 topologies as the structural targets for the development of telomere-specific G4 ligands.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

HAENSEL, Robert, Frank LOEHR, Lukáš TRANTÍREK a Volker DOETSCH. High-Resolution Insight into G-Overhang Architecture. Journal of the American Chemical Society. Washington: American Chemical Society, 2013, roč. 135, č. 7, s. 2816-2824. ISSN 0002-7863. Dostupné z: https://dx.doi.org/10.1021/ja312403b.

@article{1169515,
   author = {Haensel, Robert and Loehr, Frank and Trantírek, Lukáš and Doetsch, Volker},
   article_location = {Washington},
   article_number = {7},
   doi = {http://dx.doi.org/10.1021/ja312403b},
   keywords = {HUMAN TELOMERIC DNA; INTRAMOLECULAR G-QUADRUPLEX; K+ SOLUTION; NMR-SPECTROSCOPY; SMALL-MOLECULE; HUMAN CANCER; PULSED EPR; STABILITY; SEQUENCE; CONFORMATIONS},
   language = {eng},
   issn = {0002-7863},
   journal = {Journal of the American Chemical Society},
   title = {High-Resolution Insight into G-Overhang Architecture},
   volume = {135},
   year = {2013}
}

TY  - JOUR
ID  - 1169515
AU  - Haensel, Robert - Loehr, Frank - Trantírek, Lukáš - Doetsch, Volker
PY  - 2013
TI  - High-Resolution Insight into G-Overhang Architecture
JF  - Journal of the American Chemical Society
VL  - 135
IS  - 7
SP  - 2816-2824
EP  - 2816-2824
PB  - American Chemical Society
SN  - 00027863
KW  - HUMAN TELOMERIC DNA
KW  - INTRAMOLECULAR G-QUADRUPLEX
KW  - K+ SOLUTION
KW  - NMR-SPECTROSCOPY
KW  - SMALL-MOLECULE
KW  - HUMAN CANCER
KW  - PULSED EPR
KW  - STABILITY
KW  - SEQUENCE
KW  - CONFORMATIONS
N2  - NMR and fluorescence spectroscopy were used to address the effect of intracellular molecular crowding and related hydration on a model telomeric G-quadruplex (G4) DNA structure (d(AG(3)(TTAGGG)(3))). d(AG(3)(TTAGGG)(3)) prevalently adopted the hybrid-1 conformation in vivo, ex vivo, and in dilute potassium-based solution, while it formed the parallel propeller fold in water-depleted potassium-based solution, a commonly used model system for studying intracellular molecular crowding. The dilute potassium-based solution appeared to imitate the properties of the cellular environment required for d(AG(3)(TTAGGG)(3)) folding under in vivo and ex vivo conditions. High-resolution NMR investigations of site-specifically N-15-labeled G4 units in native-like single-stranded telomeric DNA revealed that the 3'-terminal and internal G4 unit predominantly coexist in 2-tetrad antiparallel basket and hybrid-2 structures that are arranged in "beads-on-a-string"-like fashion. Our data provide the first high-resolution insight into the telomeric G-overhang architecture under essentially physiological conditions and identify the 2-tetrad antiparallel basket and hybrid-2 topologies as the structural targets for the development of telomere-specific G4 ligands.
ER  -

HAENSEL, Robert, Frank LOEHR, Lukáš TRANTÍREK a Volker DOETSCH. High-Resolution Insight into G-Overhang Architecture. \textit{Journal of the American Chemical Society}. Washington: American Chemical Society, 2013, roč.~135, č.~7, s.~2816-2824. ISSN~0002-7863. Dostupné z: https://dx.doi.org/10.1021/ja312403b.

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