| HAENSEL, Robert, Frank LOEHR, Lukáš TRANTÍREK and Volker DOETSCH. High-Resolution Insight into G-Overhang Architecture. Journal of the American Chemical Society. Washington: American Chemical Society, 2013, vol. 135, No 7, p. 2816-2824. ISSN 0002-7863. Available from: https://dx.doi.org/10.1021/ja312403b. |
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@article{1169515,
author = {Haensel, Robert and Loehr, Frank and Trantírek, Lukáš and Doetsch, Volker},
article_location = {Washington},
article_number = {7},
doi = {http://dx.doi.org/10.1021/ja312403b},
keywords = {HUMAN TELOMERIC DNA; INTRAMOLECULAR G-QUADRUPLEX; K+ SOLUTION; NMR-SPECTROSCOPY; SMALL-MOLECULE; HUMAN CANCER; PULSED EPR; STABILITY; SEQUENCE; CONFORMATIONS},
language = {eng},
issn = {0002-7863},
journal = {Journal of the American Chemical Society},
title = {High-Resolution Insight into G-Overhang Architecture},
volume = {135},
year = {2013}
}
TY - JOUR
ID - 1169515
AU - Haensel, Robert - Loehr, Frank - Trantírek, Lukáš - Doetsch, Volker
PY - 2013
TI - High-Resolution Insight into G-Overhang Architecture
JF - Journal of the American Chemical Society
VL - 135
IS - 7
SP - 2816-2824
EP - 2816-2824
PB - American Chemical Society
SN - 00027863
KW - HUMAN TELOMERIC DNA
KW - INTRAMOLECULAR G-QUADRUPLEX
KW - K+ SOLUTION
KW - NMR-SPECTROSCOPY
KW - SMALL-MOLECULE
KW - HUMAN CANCER
KW - PULSED EPR
KW - STABILITY
KW - SEQUENCE
KW - CONFORMATIONS
N2 - NMR and fluorescence spectroscopy were used to address the effect of intracellular molecular crowding and related hydration on a model telomeric G-quadruplex (G4) DNA structure (d(AG(3)(TTAGGG)(3))). d(AG(3)(TTAGGG)(3)) prevalently adopted the hybrid-1 conformation in vivo, ex vivo, and in dilute potassium-based solution, while it formed the parallel propeller fold in water-depleted potassium-based solution, a commonly used model system for studying intracellular molecular crowding. The dilute potassium-based solution appeared to imitate the properties of the cellular environment required for d(AG(3)(TTAGGG)(3)) folding under in vivo and ex vivo conditions. High-resolution NMR investigations of site-specifically N-15-labeled G4 units in native-like single-stranded telomeric DNA revealed that the 3'-terminal and internal G4 unit predominantly coexist in 2-tetrad antiparallel basket and hybrid-2 structures that are arranged in "beads-on-a-string"-like fashion. Our data provide the first high-resolution insight into the telomeric G-overhang architecture under essentially physiological conditions and identify the 2-tetrad antiparallel basket and hybrid-2 topologies as the structural targets for the development of telomere-specific G4 ligands.
ER -
HAENSEL, Robert, Frank LOEHR, Lukáš TRANTÍREK and Volker DOETSCH. High-Resolution Insight into G-Overhang Architecture. \textit{Journal of the American Chemical Society}. Washington: American Chemical Society, 2013, vol.~135, No~7, p.~2816-2824. ISSN~0002-7863. Available from: https://dx.doi.org/10.1021/ja312403b.
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