Do cyanobacteria produce estrogenic compounds?

a 2012

Do cyanobacteria produce estrogenic compounds?

HILSCHEROVÁ, Klára, Kateřina NOVÁKOVÁ, Eliška SYCHROVÁ, Tereza ŠTĚPÁNKOVÁ, J .P. GIESY et. al.

Základní údaje

Originální název

Do cyanobacteria produce estrogenic compounds?

Autoři

HILSCHEROVÁ, Klára (203 Česká republika, garant, domácí), Kateřina NOVÁKOVÁ (203 Česká republika, domácí), Eliška SYCHROVÁ (203 Česká republika, domácí), Tereza ŠTĚPÁNKOVÁ (203 Česká republika, domácí), J .P. GIESY (124 Kanada) a Luděk BLÁHA (203 Česká republika, domácí)

Vydání

SETAC Europe 22nd Annual Meeting, Berlin, Germany, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10511 Environmental sciences

Stát vydavatele

Belgie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/12:00067012

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Cyanobacteria; Estrogenic activity

Příznaky

Mezinárodní význam

Změněno: 21. 3. 2014 09:57, Mgr. Lucie Bláhová, Ph.D.

Anotace

V originále

Number of studies document hazardous potential of cyanobacterial metabolites.Despite the observed effects on reproduction related parameters, growth and development,there is little information on endocrine disruptive potential of compounds from cyanobacteria.Our research aimed to determine if complex water bloom of cyanobacteria collected from the environment contain compounds that would interact with the signaling pathways of arylhydrocarbon receptor (AhR), androgenic receptor (AR), estrogenic receptor (ER),glucocorticoid receptor (GR) or retinoid acid receptor (RAR).In vitro reporter gene trans-activation assays were used to determine the potency of samples to elicit receptor-mediated responses.Estrogenicity was detected for most environmental samples, their estimated estrogenic equivalents ranged from 19 to 2200 ng 17b-estradiol /g dw.Some of them also interfered with the signalling of androgen receptor.The follow-up study investigated in greater detail estrogenic potency of both inner content and exudates of individual cyanobacteria and algae cultured in laboratory.The results document estrogenic potential of compounds produced by cyanobacteria and algae, generally more for the compounds excreted to their surroundings.Exudates from two algal species and from most tested pure strains of cyanobacteria elicited estrogenic activity with concentrations of estrogenic equivalents in the range of ng/L, which could be considered relatively large since equivalent potencies of estrogens have been shown to cause reproductive toxicity to aquatic animals.Aqueous extracts caused significant estrogenicity only in case of both algal species and one cyanobacteria(Aphanizomenon gracile) with estrogenic equivalents ranging from 15 to 280 ng 17b-estradiol/g DW.There does not seem to be any clear relation between the estrogenic potency and concentration of known cyanotoxins.

Návaznosti

ED0001/01/01, projekt VaV

Název: CETOCOEN

GAP503/12/0553, projekt VaV

Název: Metabolity sinic jako potenciální endokrinní disruptory

Investor: Grantová agentura ČR, Metabolity sinic jako potenciální endokrinní disruptory

Citovat

HILSCHEROVÁ, Klára, Kateřina NOVÁKOVÁ, Eliška SYCHROVÁ, Tereza ŠTĚPÁNKOVÁ, J .P. GIESY a Luděk BLÁHA. Do cyanobacteria produce estrogenic compounds? In SETAC Europe 22nd Annual Meeting, Berlin, Germany. 2012.

@proceedings{1170199,
   author = {Hilscherová, Klára and Nováková, Kateřina and Sychrová, Eliška and Štěpánková, Tereza and Giesy, J .P. and Bláha, Luděk},
   booktitle = {SETAC Europe 22nd Annual Meeting, Berlin, Germany},
   keywords = {Cyanobacteria; Estrogenic activity},
   language = {eng},
   title = {Do cyanobacteria produce estrogenic compounds?},
   year = {2012}
}

TY  - CONF
ID  - 1170199
AU  - Hilscherová, Klára - Nováková, Kateřina - Sychrová, Eliška - Štěpánková, Tereza - Giesy, J .P. - Bláha, Luděk
PY  - 2012
TI  - Do cyanobacteria produce estrogenic compounds?
KW  - Cyanobacteria
KW  - Estrogenic activity
N2  - Number of studies document hazardous potential of cyanobacterial metabolites.Despite the observed effects on reproduction related parameters, growth and development,there is little information on endocrine disruptive potential of compounds from cyanobacteria.Our research aimed to determine if complex water bloom of cyanobacteria collected from the environment contain compounds that would interact with the signaling pathways of arylhydrocarbon receptor (AhR), androgenic receptor (AR), estrogenic receptor (ER),glucocorticoid receptor (GR) or retinoid acid receptor (RAR).In vitro reporter gene trans-activation assays were used to determine the potency of samples to elicit receptor-mediated responses.Estrogenicity was detected for most environmental samples, their estimated estrogenic equivalents ranged from 19 to 2200 ng 17b-estradiol /g dw.Some of them also interfered with the signalling of androgen receptor.The follow-up study investigated in greater detail estrogenic potency of both inner content and exudates of individual cyanobacteria and algae cultured in laboratory.The results document estrogenic potential of compounds produced by cyanobacteria and algae, generally more for the compounds excreted to their surroundings.Exudates from two algal species and from most tested pure strains of cyanobacteria elicited estrogenic activity with concentrations of estrogenic equivalents in the range of ng/L, which could be considered relatively large since equivalent potencies of estrogens have been shown to cause reproductive toxicity to aquatic animals.Aqueous extracts caused significant estrogenicity only in case of both algal species and one cyanobacteria(Aphanizomenon gracile) with estrogenic equivalents ranging from 15 to 280 ng 17b-estradiol/g DW.There does not seem to be any clear relation between the estrogenic potency and concentration of known cyanotoxins.
ER  -

HILSCHEROVÁ, Klára, Kateřina NOVÁKOVÁ, Eliška SYCHROVÁ, Tereza ŠTĚPÁNKOVÁ, J .P. GIESY a Luděk BLÁHA. Do cyanobacteria produce estrogenic compounds? In \textit{SETAC Europe 22nd Annual Meeting, Berlin, Germany}. 2012.

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