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@proceedings{1173335,
author = {Rájecký, Michal and Táborská, Eva and Táborský, Petr},
booktitle = {European symposium on atomic spectrometry ESAS 2014 & 15th Czech-Slovak spectroscopic conference},
keywords = {benzo[c]phenanthridine alkaloids; fluorescence; lifetime; quenching; DNA},
language = {eng},
isbn = {978-80-905704-1-2},
title = {Quaternary benzo[c]phenantrhidine alkaloids - spectroscopic properties},
year = {2014}
}
TY - CONF
ID - 1173335
AU - Rájecký, Michal - Táborská, Eva - Táborský, Petr
PY - 2014
TI - Quaternary benzo[c]phenantrhidine alkaloids - spectroscopic properties
SN - 9788090570412
KW - benzo[c]phenanthridine alkaloids
KW - fluorescence
KW - lifetime
KW - quenching
KW - DNA
N2 - Quaternary benzo[c]phenanthridine alkaloids (QBAs) form a subgroup of isoquinoline alkaloids with various effects to cell functions, ranging from antibacterial to proapoptotic effect. In this work we present fluorescent properties of several QBAs isolated from plant material (so called minor QBAs, because they are present in low amount in plant) and compare them with commercially available QBAs sanguinarine and chelerythine. QBAs exist in two forms depending on the pH of a solution. In basic media QBAs (alkanolamine form) produce blue fluorescence with slightly longer lifetime while in acidic media (iminium form) fluorescence is red and much less intense (especially true for minor QBAs). The iminium form interacts with double-stranded DNA which results in an enhancement of red fluorescence (with exception of sanguinarine). This could partially be explained by quenching of red fluorescence of free QBAs by water molecules, because fluorescence in H2O is more quenched than in D2O.
ER -
RÁJECKÝ, Michal, Eva TÁBORSKÁ and Petr TÁBORSKÝ. Quaternary benzo[c]phenantrhidine alkaloids - spectroscopic properties. In \textit{European symposium on atomic spectrometry ESAS 2014 \&{} 15th Czech-Slovak spectroscopic conference}. 2014. ISBN~978-80-905704-1-2.
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