Study of the retention behavior of trinucleotides in HPLC

D 2014

Study of the retention behavior of trinucleotides in HPLC

MIKULÁŠEK, Kamil, Miroslava BITTOVÁ a Jan HAVLIŠ

Základní údaje

Originální název

Study of the retention behavior of trinucleotides in HPLC

Název česky

Studium retenčního chování trinukleotidů pomocí HPLC

Autoři

MIKULÁŠEK, Kamil (203 Česká republika), Miroslava BITTOVÁ (203 Česká republika) a Jan HAVLIŠ (203 Česká republika, garant, domácí)

Vydání

Olomouc, Acta Universitatis Palackianae Olomucensis Chemica 51S, od s. 161-162, 2 s. 2014

Nakladatel

Palacký University, Olomouc

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Kód RIV

RIV/00216224:14310/14:00075244

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-244-3950-1

ISSN

Klíčová slova česky

trinukleotidy; RP-HPLC

Klíčová slova anglicky

trinucleotides; RP-HPLC

Změněno: 14. 4. 2014 21:35, Mgr. Miroslava Bittová, Ph.D.

Anotace

V originále

Trinucleotides are short DNA sequences of three nucleobases; in our study we used guanine, adenine and thymine. It was shown that a useful tool to study the effect of sequence on retention was achieved by reversed phase liquid chromatography in ion-interaction mode (II-RPLC). Retention mechanism in the II-RPLC is based on addition of the ion interaction agent (in a form of buffer ions) to the mobile phase, which can effectively compensate the charge on the phosphate groups of a sugar-phosphate backbone, thereby increasing the hydrophobicity and thus the retention in system with reversed phase. To study the retention behavior we have chosen a combination of experimental design (ED) and artificial neural networks (ANN). ED was used to target more variable parameters in parallel while monitoring the system response. This method allows to determine the size of the effect of the various factors involved in the retention and to possibly reveal their mutual correlation. It is commonly connected to approximation of the response function using artificial neural networks (ANN), which then enables prediction of the retention time of an unknown sequence. This approach could be used as a tool to identify unknown short nucleotide sequences in mixtures

Návaznosti

MUNI/A/0972/2013, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2014 (Akronym: MIAB)

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2014, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

MIKULÁŠEK, Kamil, Miroslava BITTOVÁ a Jan HAVLIŠ. Study of the retention behavior of trinucleotides in HPLC. In Acta Universitatis Palackianae Olomucensis Chemica 51S. Olomouc: Palacký University, Olomouc, 2014, s. 161-162. ISBN 978-80-244-3950-1.

@inproceedings{1173602,
   author = {Mikulášek, Kamil and Bittová, Miroslava and Havliš, Jan},
   address = {Olomouc},
   booktitle = {Acta Universitatis Palackianae Olomucensis Chemica 51S},
   keywords = {trinucleotides; RP-HPLC},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Olomouc},
   isbn = {978-80-244-3950-1},
   pages = {161-162},
   publisher = {Palacký University, Olomouc},
   title = {Study of the retention behavior of trinucleotides in HPLC},
   year = {2014}
}

TY  - JOUR
ID  - 1173602
AU  - Mikulášek, Kamil - Bittová, Miroslava - Havliš, Jan
PY  - 2014
TI  - Study of the retention behavior of trinucleotides in HPLC
PB  - Palacký University, Olomouc
CY  - Olomouc
SN  - 9788024439501
KW  - trinucleotides
KW  - RP-HPLC
N2  - Trinucleotides are short DNA sequences of three nucleobases; in our study we used guanine, adenine and thymine. It was shown that a useful tool to study the effect of sequence on retention was achieved by reversed phase liquid chromatography in ion-interaction mode (II-RPLC). Retention mechanism in the II-RPLC is based on addition of the ion interaction agent (in a form of buffer ions) to the mobile phase, which can effectively compensate the charge on the phosphate groups of a sugar-phosphate backbone, thereby increasing the hydrophobicity and thus the retention in system with reversed phase. To study the retention behavior we have chosen a combination of experimental design (ED) and artificial neural networks (ANN). ED was used to target more variable parameters in parallel while monitoring the system response. This method allows to determine the size of the effect of the various factors involved in the retention and to possibly reveal their mutual correlation. It is commonly connected to approximation of the response function using artificial neural networks (ANN), which then enables prediction of the retention time of an unknown sequence. This approach could be used as a tool to identify unknown short nucleotide sequences in mixtures
ER  -

MIKULÁŠEK, Kamil, Miroslava BITTOVÁ a Jan HAVLIŠ. Study of the retention behavior of trinucleotides in HPLC. In \textit{Acta Universitatis Palackianae Olomucensis Chemica 51S}. Olomouc: Palacký University, Olomouc, 2014, s.~161-162. ISBN~978-80-244-3950-1.

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