Acute, chronic and reproductive toxicity of complex
cyanobacterial blooms in Daphnia magna and the role of
microcystins

J 2014

Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins

SMUTNÁ, Marie, Pavel BABICA, Sergio JARQUE ORTIZ, Klára HILSCHEROVÁ, Blahoslav MARŠÁLEK et. al.

Základní údaje

Originální název

Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins

Autoři

SMUTNÁ, Marie (203 Česká republika, domácí), Pavel BABICA (203 Česká republika, domácí), Sergio JARQUE ORTIZ (724 Španělsko, domácí), Klára HILSCHEROVÁ (203 Česká republika, domácí), Blahoslav MARŠÁLEK (203 Česká republika, domácí), Maher HAEBA (434 Libye, domácí) a Luděk BLÁHA (203 Česká republika, garant, domácí)

Vydání

Toxicon, OXFORD, Elsevier Science, 2014, 0041-0101

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.492

Kód RIV

RIV/00216224:14310/14:00073577

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.toxicon.2013.12.009

UT WoS

000332444100002

Klíčová slova anglicky

Cyanotoxin; Reproduction; Cyanobacterial bloom; Daphnia magna; Microcystin

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 1. 2015 15:32, Ing. Andrea Mikešková

Anotace

V originále

Ecotoxicity of toxins from cyanobacteria such as microcystins has been studied extensively, little is known about the risks they pose in the wild, i.e. within complex biomasses. In this work, crustaceans (Daphnia magna) were exposed to varying concentrations (0-405 mg d.w L-1) of eight complex cyanobacterial water bloom samples in a series of acute (48 h) and chronic (21 day) toxicity experiments. Further acute and chronic exposure assays were performed using aqueous extracts of the crude biomass samples and two fractions prepared by solid phase extraction (SPE) of the aqueous extracts. High acute toxicity was observed for 6 of the 8 crude biomass samples. Chronic exposure assays were performed using one complex biomass sample and its various subsamples/fractions. The complex biomass, the crude aqueous extract, and the microcystin-free SPE permeate all elicited similar and significant lethal effects, with LC50 values of around 35.6 mg biomass d.w L-1 after 21 days. The cyanobacterial biomass samples also affected reproductive health, significantly increasing the time to the first brood (LOEC = 45 mg center dot d.w L-1 exposure) and inhibiting fecundity by 50% at 15 mg d.w L-1. Conversely, the microcystin-containing C18-SPE eluate fraction had only weak effects in the chronic assay. These results indicate that cyanobacterial water blooms are highly toxic to zooplankton (both acutely and chronically) at environmentally relevant concentrations. However, the effects observed in the acute and chronic assays were independent of the samples' microcystin contents. Our results thus point out the importance of other cyanobacterial components such as lipopolysaccharides, various peptides and depsipeptides, polar alkaloid metabolites or other unidentified metabolites in the overall ecotoxicity of complex cyanobacterial blooms.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP503/12/0553, projekt VaV

Název: Metabolity sinic jako potenciální endokrinní disruptory

Investor: Grantová agentura ČR, Metabolity sinic jako potenciální endokrinní disruptory

Citovat

SMUTNÁ, Marie, Pavel BABICA, Sergio JARQUE ORTIZ, Klára HILSCHEROVÁ, Blahoslav MARŠÁLEK, Maher HAEBA a Luděk BLÁHA. Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins. Toxicon. OXFORD: Elsevier Science, 2014, roč. 79, march, s. 11-18. ISSN 0041-0101. Dostupné z: https://dx.doi.org/10.1016/j.toxicon.2013.12.009.

@article{1174154,
   author = {Smutná, Marie and Babica, Pavel and Jarque Ortiz, Sergio and Hilscherová, Klára and Maršálek, Blahoslav and Haeba, Maher and Bláha, Luděk},
   article_location = {OXFORD},
   article_number = {march},
   doi = {http://dx.doi.org/10.1016/j.toxicon.2013.12.009},
   keywords = {Cyanotoxin; Reproduction; Cyanobacterial bloom; Daphnia magna; Microcystin},
   language = {eng},
   issn = {0041-0101},
   journal = {Toxicon},
   title = {Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins},
   volume = {79},
   year = {2014}
}

TY  - JOUR
ID  - 1174154
AU  - Smutná, Marie - Babica, Pavel - Jarque Ortiz, Sergio - Hilscherová, Klára - Maršálek, Blahoslav - Haeba, Maher - Bláha, Luděk
PY  - 2014
TI  - Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins
JF  - Toxicon
VL  - 79
IS  - march
SP  - 11-18
EP  - 11-18
PB  - Elsevier Science
SN  - 00410101
KW  - Cyanotoxin
KW  - Reproduction
KW  - Cyanobacterial bloom
KW  - Daphnia magna
KW  - Microcystin
N2  - Ecotoxicity of toxins from cyanobacteria such as microcystins has been studied extensively, little is known about the risks they pose in the wild, i.e. within complex biomasses. In this work, crustaceans (Daphnia magna) were exposed to varying concentrations (0-405 mg d.w L-1) of eight complex cyanobacterial water bloom samples in a series of acute (48 h) and chronic (21 day) toxicity experiments. Further acute and chronic exposure assays were performed using aqueous extracts of the crude biomass samples and two fractions prepared by solid phase extraction (SPE) of the aqueous extracts. High acute toxicity was observed for 6 of the 8 crude biomass samples. Chronic exposure assays were performed using one complex biomass sample and its various subsamples/fractions. The complex biomass, the crude aqueous extract, and the microcystin-free SPE permeate all elicited similar and significant lethal effects, with LC50 values of around 35.6 mg biomass d.w L-1 after 21 days. The cyanobacterial biomass samples also affected reproductive health, significantly increasing the time to the first brood (LOEC = 45 mg center dot d.w L-1 exposure) and inhibiting fecundity by 50% at 15 mg d.w L-1. Conversely, the microcystin-containing C18-SPE eluate fraction had only weak effects in the chronic assay. These results indicate that cyanobacterial water blooms are highly toxic to zooplankton (both acutely and chronically) at environmentally relevant concentrations. However, the effects observed in the acute and chronic assays were independent of the samples' microcystin contents. Our results thus point out the importance of other cyanobacterial components such as lipopolysaccharides, various peptides and depsipeptides, polar alkaloid metabolites or other unidentified metabolites in the overall ecotoxicity of complex cyanobacterial blooms.
ER  -

SMUTNÁ, Marie, Pavel BABICA, Sergio JARQUE ORTIZ, Klára HILSCHEROVÁ, Blahoslav MARŠÁLEK, Maher HAEBA a Luděk BLÁHA. Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins. \textit{Toxicon}. OXFORD: Elsevier Science, 2014, roč.~79, march, s.~11-18. ISSN~0041-0101. Dostupné z: https://dx.doi.org/10.1016/j.toxicon.2013.12.009.

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