Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne
asmulti-redox centre DNA label at mercury meniscus modified
silversolid amalgam electrode

J 2014

Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode

DAŇHEL, Aleš, Veronika RAINDLOVA, Luděk HAVRAN, Hana PIVOŇKOVÁ, Michal HOCEK et. al.

Základní údaje

Originální název

Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode

Autoři

DAŇHEL, Aleš (203 Česká republika), Veronika RAINDLOVA (203 Česká republika), Luděk HAVRAN (203 Česká republika), Hana PIVOŇKOVÁ (203 Česká republika), Michal HOCEK (203 Česká republika) a Miroslav FOJTA (203 Česká republika, garant, domácí)

Vydání

Electrochimica Acta, OXFORD, Elsevier Science Ltd, 2014, 0013-4686

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.504

Kód RIV

RIV/00216224:14740/14:00073589

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.electacta.2013.09.147

UT WoS

000336340900016

Klíčová slova anglicky

DNA; Redox labeling; 2_4-Dinitrophenylhydrazine; Silver solid amalgam electrode; Voltammetry

Štítky

DNA, kontrola MP, rivok, Voltammetry

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2015 08:30, Martina Prášilová

Anotace

V originále

A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications.

Návaznosti

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

DAŇHEL, Aleš, Veronika RAINDLOVA, Luděk HAVRAN, Hana PIVOŇKOVÁ, Michal HOCEK a Miroslav FOJTA. Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode. Electrochimica Acta. OXFORD: Elsevier Science Ltd, 2014, roč. 126, SI, s. 122-131. ISSN 0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2013.09.147.

@article{1179581,
   author = {Daňhel, Aleš and Raindlova, Veronika and Havran, Luděk and Pivoňková, Hana and Hocek, Michal and Fojta, Miroslav},
   article_location = {OXFORD},
   article_number = {SI},
   doi = {http://dx.doi.org/10.1016/j.electacta.2013.09.147},
   keywords = {DNA; Redox labeling; 2_4-Dinitrophenylhydrazine; Silver solid amalgam electrode; Voltammetry},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode},
   url = {http://dx.doi.org/10.1016/j.electacta.2013.09.147},
   volume = {126},
   year = {2014}
}

TY  - JOUR
ID  - 1179581
AU  - Daňhel, Aleš - Raindlova, Veronika - Havran, Luděk - Pivoňková, Hana - Hocek, Michal - Fojta, Miroslav
PY  - 2014
TI  - Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode
JF  - Electrochimica Acta
VL  - 126
IS  - SI
SP  - 122-131
EP  - 122-131
PB  - Elsevier Science Ltd
SN  - 00134686
KW  - DNA
KW  - Redox labeling
KW  - 2_4-Dinitrophenylhydrazine
KW  - Silver solid amalgam electrode
KW  - Voltammetry
UR  - http://dx.doi.org/10.1016/j.electacta.2013.09.147
L2  - http://dx.doi.org/10.1016/j.electacta.2013.09.147
N2  - A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications.
ER  -

DAŇHEL, Aleš, Veronika RAINDLOVA, Luděk HAVRAN, Hana PIVOŇKOVÁ, Michal HOCEK a Miroslav FOJTA. Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode. \textit{Electrochimica Acta}. OXFORD: Elsevier Science Ltd, 2014, roč.~126, SI, s.~122-131. ISSN~0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2013.09.147.

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