RAINDLOVA, Veronika, Radek POHL, Blanka KLEPETAROVA, Luděk HAVRAN, Eva SIMKOVA, Petra HORAKOVA, Hana PIVOŇKOVÁ, Miroslav FOJTA a Michal HOCEK. Synthesis of Hydrazone-Modified Nucleotides and Their Polymerase Incorporation onto DNA for Redox Labeling. CHEMPLUSCHEM. Weinheim: Wiley - Verlag Chemie, 2012, roč. 77, č. 8, s. 652-662. ISSN 2192-6506. Dostupné z: https://dx.doi.org/10.1002/cplu.201200056. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1179811, author = {Raindlova, Veronika and Pohl, Radek and Klepetarova, Blanka and Havran, Luděk and Simkova, Eva and Horakova, Petra and Pivoňková, Hana and Fojta, Miroslav and Hocek, Michal}, article_location = {Weinheim}, article_number = {8}, doi = {http://dx.doi.org/10.1002/cplu.201200056}, keywords = {cross-coupling; DNA; electrochemistry; hydrazones; nucleotides; polymerases}, language = {eng}, issn = {2192-6506}, journal = {CHEMPLUSCHEM}, title = {Synthesis of Hydrazone-Modified Nucleotides and Their Polymerase Incorporation onto DNA for Redox Labeling}, volume = {77}, year = {2012} }
TY - JOUR ID - 1179811 AU - Raindlova, Veronika - Pohl, Radek - Klepetarova, Blanka - Havran, Luděk - Simkova, Eva - Horakova, Petra - Pivoňková, Hana - Fojta, Miroslav - Hocek, Michal PY - 2012 TI - Synthesis of Hydrazone-Modified Nucleotides and Their Polymerase Incorporation onto DNA for Redox Labeling JF - CHEMPLUSCHEM VL - 77 IS - 8 SP - 652-662 EP - 652-662 PB - Wiley - Verlag Chemie SN - 21926506 KW - cross-coupling KW - DNA KW - electrochemistry KW - hydrazones KW - nucleotides KW - polymerases N2 - 5-(5-Formylthiophen-2-yl)cytosine, 7-(5-formylthiophen-2-yl)-7-deazaadenosine 2'-deoxyribonucleosides, and their 5'-O-triphosphates (dNTPs) were converted into the 2,4-dinitrophenylhydrazone or nitrobenzofurazanyl. The hydrazone-modified dNTPs were enzymatically incorporated into DNA by polymerase catalyzed primer extension (PEX). This direct incorporation of hydrazone-linked dNTPs was compared to previously reported incorporation of aldehyde-modified dNTPs followed by postsynthetic hydrazone formation on DNA to show that the direct incorporation can be used for incorporation of more hydrazone units, however, cleaner PEX products are formed by incorporation of aldehydes and subsequent reaction with hydrazines. Extensive study of electrochemical behavior of the nitroarylhydrazone-linked nucleosides and DNA was performed confirming the potential utility of the hydrazone, nitroaryl, and benzofurazane groups for redox labeling of DNA. ER -
RAINDLOVA, Veronika, Radek POHL, Blanka KLEPETAROVA, Luděk HAVRAN, Eva SIMKOVA, Petra HORAKOVA, Hana PIVOŇKOVÁ, Miroslav FOJTA a Michal HOCEK. Synthesis of Hydrazone-Modified Nucleotides and Their Polymerase Incorporation onto DNA for Redox Labeling. \textit{CHEMPLUSCHEM}. Weinheim: Wiley - Verlag Chemie, 2012, roč.~77, č.~8, s.~652-662. ISSN~2192-6506. Dostupné z: https://dx.doi.org/10.1002/cplu.201200056.
|