Cystatins of the parasitic nematodes from the genus Trichinella

k 2014

Cystatins of the parasitic nematodes from the genus Trichinella

ŠKORPÍKOVÁ, Lucie, Eliška ŠRÁMOVÁ, Jana ILGOVÁ, Břetislav KOUDELA, Milan GELNAR et. al.

Základní údaje

Originální název

Cystatins of the parasitic nematodes from the genus Trichinella

Autoři

ŠKORPÍKOVÁ, Lucie (203 Česká republika, garant, domácí), Eliška ŠRÁMOVÁ (203 Česká republika, domácí), Jana ILGOVÁ (703 Slovensko, domácí), Břetislav KOUDELA (203 Česká republika), Milan GELNAR (203 Česká republika, domácí) a Martin KAŠNÝ (203 Česká republika, domácí)

Vydání

21st Helminthological Days, 2014

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/14:00075464

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-6796-7

Klíčová slova anglicky

Trichinella; multi cystatin-like domain gene; Trichinella spiralis; Trichinella britovi; Trichinella pseudospiralis

Změněno: 16. 10. 2015 09:59, Mgr. Lucie Škorpíková, Ph.D.

Anotace

V originále

The nematodes of the genus Trichinella are intracellular parasites of small intestine and skeletal muscle cells. They can infect a broad range of worldwide distributed hosts like birds, reptiles and mammals. All Trichinella spp. are zoonotic but only six of twelve genotypes have been detected in humans, causing the trichinellosis - a serious, sometimes fatal, human disease, which has been documented in 55 countries of the World. Our study is focused on characterization of composition of excretory-secretory products (ESP) from L1 muscle larvae of three trichinella species - Trichinella spiralis, T. britovi and T. pseudospiralis. The dominant components of L1 ESP are cystatins. These proteins are a reversible inhibitors of proteolytics enzymes - cystein peptideases and they are involved in many relevant biological processes such as development, growth, digestion and migration of parasites and suppression of host immune responses. The main aim of our study is to prepare the recombinant form of three trichinella cystatin and compare/characterize their properties. We used primers specifically designed for identified “multi cystatin-like domain” gene of T. spiralis. Subsequently, we obtained sequences of this gene for the other two species and we compared these data. We found out that cystatin genes of T. spiralis and T. britovi have high sequence homology although gene of T. pseudospiralis differs more (e.g. number of domains, conserved motifs).

Návaznosti

MUNI/A/0888/2013, interní kód MU

Název: Biodiverzita - analýzy biologických systémů různých úrovní a na různých škálách prostředí (Akronym: BIDA3)

Investor: Masarykova univerzita, Biodiverzita - analýzy biologických systémů různých úrovní a na různých škálách prostředí, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

ŠKORPÍKOVÁ, Lucie, Eliška ŠRÁMOVÁ, Jana ILGOVÁ, Břetislav KOUDELA, Milan GELNAR a Martin KAŠNÝ. Cystatins of the parasitic nematodes from the genus Trichinella. In 21st Helminthological Days. 2014. ISBN 978-80-210-6796-7.

@proceedings{1182594,
   author = {Škorpíková, Lucie and Šrámová, Eliška and Ilgová, Jana and Koudela, Břetislav and Gelnar, Milan and Kašný, Martin},
   booktitle = {21st Helminthological Days},
   keywords = {Trichinella; multi cystatin-like domain gene; Trichinella spiralis; Trichinella britovi; Trichinella pseudospiralis},
   language = {eng},
   isbn = {978-80-210-6796-7},
   title = {Cystatins of the parasitic nematodes from the genus Trichinella},
   year = {2014}
}

TY  - CONF
ID  - 1182594
AU  - Škorpíková, Lucie - Šrámová, Eliška - Ilgová, Jana - Koudela, Břetislav - Gelnar, Milan - Kašný, Martin
PY  - 2014
TI  - Cystatins of the parasitic nematodes from the genus Trichinella
SN  - 9788021067967
KW  - Trichinella
KW  - multi cystatin-like domain gene
KW  - Trichinella spiralis
KW  - Trichinella britovi
KW  - Trichinella pseudospiralis
N2  - The nematodes of the genus Trichinella are intracellular parasites of small intestine and skeletal muscle cells. They can infect a broad range of worldwide distributed hosts like birds, reptiles and mammals. All Trichinella spp. are zoonotic but only six of twelve genotypes have been detected in humans, causing the trichinellosis - a serious, sometimes fatal, human disease, which has been documented in 55 countries of the World. Our study is focused on characterization of composition of excretory-secretory products (ESP) from L1 muscle larvae of three trichinella species - Trichinella spiralis, T. britovi and T. pseudospiralis. The dominant components of L1 ESP are cystatins. These proteins are a reversible inhibitors of proteolytics enzymes - cystein peptideases and they are involved in many relevant biological processes such as development, growth, digestion and migration of parasites and suppression of host immune responses. The main aim of our study is to prepare the recombinant form of three trichinella cystatin and compare/characterize their properties. We used primers specifically designed for identified “multi cystatin-like domain” gene of T. spiralis. Subsequently, we obtained sequences of this gene for the other two species and we compared these data. We found out that cystatin genes of T. spiralis and T. britovi have high sequence homology although gene of T. pseudospiralis differs more (e.g. number of domains, conserved motifs).
ER  -

ŠKORPÍKOVÁ, Lucie, Eliška ŠRÁMOVÁ, Jana ILGOVÁ, Břetislav KOUDELA, Milan GELNAR a Martin KAŠNÝ. Cystatins of the parasitic nematodes from the genus Trichinella. In \textit{21st Helminthological Days}. 2014. ISBN~978-80-210-6796-7.

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