Coilin is rapidly recruited to UVA-induced DNA lesions and
gamma-radiation affects localized movement of Cajal bodies

J 2014

Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies

BÁRTOVÁ, Eva, Veronika FOLTÁNKOVÁ, Soňa LEGARTOVÁ, Petra SEHNALOVÁ, Dmitry SOROKIN et. al.

Základní údaje

Originální název

Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies

Autoři

BÁRTOVÁ, Eva (203 Česká republika), Veronika FOLTÁNKOVÁ (203 Česká republika), Soňa LEGARTOVÁ (703 Slovensko), Petra SEHNALOVÁ (203 Česká republika), Dmitry SOROKIN (643 Rusko, garant, domácí), Jana SUCHÁNKOVÁ (203 Česká republika) a Stanislav KOZUBEK (203 Česká republika)

Vydání

Nucleus, Landes Bioscience, 2014, 1949-1034

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.033

Kód RIV

RIV/00216224:14330/14:00073626

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.4161/nucl.29229

UT WoS

000341641600009

Klíčová slova anglicky

DNA repair; chromatin; nucleus; nucleolus; Cajal bodies; coilin

Štítky

CBIA, cbia-web

Změněno: 27. 4. 2015 03:55, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

Cajal bodies are important nuclear structures containing proteins that preferentially regulate RNA-related metabolism. We investigated the cell-type specific nuclear distribution of Cajal bodies and the level of coilin, a protein of Cajal bodies, in non-irradiated and irradiated human tumor cell lines and embryonic stem (ES) cells. Cajal bodies were localized in different nuclear compartments, including DAPI-poor regions, in the proximity of chromocenters, and adjacent to nucleoli. The number of Cajal bodies per nucleus was cell cycle-dependent, with higher numbers occurring during G2 phase. Human ES cells contained a high coilin level in the nucleoplasm, but coilin-positive Cajal bodies were also identified in nuclei of mouse and human ES cells. Coilin, but not SMN, recognized UVA-induced DNA lesions, which was cell cycle-independent. Treatment with gamma-radiation reduced the localized movement of Cajal bodies in many cell types and GFP-coilin fluorescence recovery after photobleaching was very fast in nucleoplasm in comparison with GFP-coilin recovery in DNA lesions. By contrast, nucleolus-localized coilin displayed very slow fluorescence recovery after photobleaching, which indicates very slow rates of protein diffusion, especially in mouse ES cell nucleoli.

Návaznosti

CZ.1.07/2.3.00/30.0030, interní kód MU

Název: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Rozvoj lidských zdrojů pro oblast buněčné biologie, 2.3 Lidské zdroje ve výzkumu a vývoji

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

BÁRTOVÁ, Eva, Veronika FOLTÁNKOVÁ, Soňa LEGARTOVÁ, Petra SEHNALOVÁ, Dmitry SOROKIN, Jana SUCHÁNKOVÁ a Stanislav KOZUBEK. Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies. Nucleus. Landes Bioscience, 2014, roč. 5, č. 3, s. 269-277. ISSN 1949-1034. Dostupné z: https://dx.doi.org/10.4161/nucl.29229.

@article{1182653,
   author = {Bártová, Eva and Foltánková, Veronika and Legartová, Soňa and Sehnalová, Petra and Sorokin, Dmitry and Suchánková, Jana and Kozubek, Stanislav},
   article_number = {3},
   doi = {http://dx.doi.org/10.4161/nucl.29229},
   keywords = {DNA repair; chromatin; nucleus; nucleolus; Cajal bodies; coilin},
   language = {eng},
   issn = {1949-1034},
   journal = {Nucleus},
   title = {Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies},
   volume = {5},
   year = {2014}
}

TY  - JOUR
ID  - 1182653
AU  - Bártová, Eva - Foltánková, Veronika - Legartová, Soňa - Sehnalová, Petra - Sorokin, Dmitry - Suchánková, Jana - Kozubek, Stanislav
PY  - 2014
TI  - Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies
JF  - Nucleus
VL  - 5
IS  - 3
SP  - 269-277
EP  - 269-277
PB  - Landes Bioscience
SN  - 19491034
KW  - DNA repair
KW  - chromatin
KW  - nucleus
KW  - nucleolus
KW  - Cajal bodies
KW  - coilin
N2  - Cajal bodies are important nuclear structures containing proteins that preferentially regulate RNA-related metabolism. We investigated the cell-type specific nuclear distribution of Cajal bodies and the level of coilin, a protein of Cajal bodies, in non-irradiated and irradiated human tumor cell lines and embryonic stem (ES) cells. Cajal bodies were localized in different nuclear compartments, including DAPI-poor regions, in the proximity of chromocenters, and adjacent to nucleoli. The number of Cajal bodies per nucleus was cell cycle-dependent, with higher numbers occurring during G2 phase. Human ES cells contained a high coilin level in the nucleoplasm, but coilin-positive Cajal bodies were also identified in nuclei of mouse and human ES cells. Coilin, but not SMN, recognized UVA-induced DNA lesions, which was cell cycle-independent. Treatment with gamma-radiation reduced the localized movement of Cajal bodies in many cell types and GFP-coilin fluorescence recovery after photobleaching was very fast in nucleoplasm in comparison with GFP-coilin recovery in DNA lesions. By contrast, nucleolus-localized coilin displayed very slow fluorescence recovery after photobleaching, which indicates very slow rates of protein diffusion, especially in mouse ES cell nucleoli.
ER  -

BÁRTOVÁ, Eva, Veronika FOLTÁNKOVÁ, Soňa LEGARTOVÁ, Petra SEHNALOVÁ, Dmitry SOROKIN, Jana SUCHÁNKOVÁ a Stanislav KOZUBEK. Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies. \textit{Nucleus}. Landes Bioscience, 2014, roč.~5, č.~3, s.~269-277. ISSN~1949-1034. Dostupné z: https://dx.doi.org/10.4161/nucl.29229.

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