The influence of culture conditions on the identification of
Mycobacterium species by MALDI-TOF MS profiling

J 2014

The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling

BALÁŽOVÁ, Tereza, Jitka MAKOVCOVÁ, Ondrej ŠEDO, Michal SLANÝ, Martin FALDYNA et. al.

Základní údaje

Originální název

The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling

Autoři

BALÁŽOVÁ, Tereza (203 Česká republika, domácí), Jitka MAKOVCOVÁ (203 Česká republika), Ondrej ŠEDO (203 Česká republika, domácí), Michal SLANÝ (203 Česká republika), Martin FALDYNA (203 Česká republika) a Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí)

Vydání

FEMS Microbiology Letters, Hoboken (USA), Elsevier Science, 2014, 0378-1097

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.121

Kód RIV

RIV/00216224:14740/14:00073252

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1111/1574-6968.12408

UT WoS

000333654500011

Klíčová slova anglicky

MALDI-TOFMS; mycobacteria profiling; strain differentiation; Mycobacterium phlei; Mycobacterium smegmatis

Štítky

kontrola MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2015 11:41, Martina Prášilová

Anotace

V originále

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MALDI-TOF MS profiling analysis. In this study, we tested two recently published protocols for inactivation and disruption of mycobacteria, and we also examined the influence of different culture conditions (four culture media and five cultivation times) on mass spectral quality and the discriminatory power of the method. We found a significant influence of sample pretreatment method and culture medium on species identification and differentiation for a total of 10 strains belonging to Mycobacterium phlei and Mycobacteriumsmegmatis. Optimum culture conditions yielding the highest identification success rate against the BioTyper database (Bruker Daltonics) and permitting the possibility of automatic acquisition of mass spectra were found to be distinct for the two mycobacterial species examined. Similarly, individual changes in growth conditions had diverse effects on the two species. For these reasons, thorough control over cultivation conditions should always be employed to maximize the performance and discriminatory power of MALDI-TOF MS profiling, and cultivation conditions must be optimized separately for individual groups of mycobacterial species/strains.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

EE2.3.20.0183, projekt VaV

Název: Centrum experimentální biomedicíny

QI101A094, projekt VaV

Název: Metody tlumení produkčních chorob skotu - BVD-MD a paratuberkulóza

Investor: Ministerstvo zemědělství ČR, Metody tlumení produkčních chorob skotu - BVD-MD a paratuberkulóza, Udržitelný rozvoj agrárního sektoru

Citovat

BALÁŽOVÁ, Tereza, Jitka MAKOVCOVÁ, Ondrej ŠEDO, Michal SLANÝ, Martin FALDYNA a Zbyněk ZDRÁHAL. The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling. FEMS Microbiology Letters. Hoboken (USA): Elsevier Science, 2014, roč. 353, č. 1, s. 77-84. ISSN 0378-1097. Dostupné z: https://dx.doi.org/10.1111/1574-6968.12408.

@article{1183302,
   author = {Balážová, Tereza and Makovcová, Jitka and Šedo, Ondrej and Slaný, Michal and Faldyna, Martin and Zdráhal, Zbyněk},
   article_location = {Hoboken (USA)},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/1574-6968.12408},
   keywords = {MALDI-TOFMS; mycobacteria profiling; strain differentiation; Mycobacterium phlei; Mycobacterium smegmatis},
   language = {eng},
   issn = {0378-1097},
   journal = {FEMS Microbiology Letters},
   title = {The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf},
   volume = {353},
   year = {2014}
}

TY  - JOUR
ID  - 1183302
AU  - Balážová, Tereza - Makovcová, Jitka - Šedo, Ondrej - Slaný, Michal - Faldyna, Martin - Zdráhal, Zbyněk
PY  - 2014
TI  - The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling
JF  - FEMS Microbiology Letters
VL  - 353
IS  - 1
SP  - 77-84
EP  - 77-84
PB  - Elsevier Science
SN  - 03781097
KW  - MALDI-TOFMS
KW  - mycobacteria profiling
KW  - strain differentiation
KW  - Mycobacterium phlei
KW  - Mycobacterium smegmatis
UR  - http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf
L2  - http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf
N2  - Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MALDI-TOF MS profiling analysis. In this study, we tested two recently published protocols for inactivation and disruption of mycobacteria, and we also examined the influence of different culture conditions (four culture media and five cultivation times) on mass spectral quality and the discriminatory power of the method. We found a significant influence of sample pretreatment method and culture medium on species identification and differentiation for a total of 10 strains belonging to Mycobacterium phlei and Mycobacteriumsmegmatis. Optimum culture conditions yielding the highest identification success rate against the BioTyper database (Bruker Daltonics) and permitting the possibility of automatic acquisition of mass spectra were found to be distinct for the two mycobacterial species examined. Similarly, individual changes in growth conditions had diverse effects on the two species. For these reasons, thorough control over cultivation conditions should always be employed to maximize the performance and discriminatory power of MALDI-TOF MS profiling, and cultivation conditions must be optimized separately for individual groups of mycobacterial species/strains.
ER  -

BALÁŽOVÁ, Tereza, Jitka MAKOVCOVÁ, Ondrej ŠEDO, Michal SLANÝ, Martin FALDYNA a Zbyněk ZDRÁHAL. The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling. \textit{FEMS Microbiology Letters}. Hoboken (USA): Elsevier Science, 2014, roč.~353, č.~1, s.~77-84. ISSN~0378-1097. Dostupné z: https://dx.doi.org/10.1111/1574-6968.12408.

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