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@article{1183882, author = {Kubáň, Petr and Ďurč, Pavol and Bittová, Miroslava and Foret, František}, article_location = {Amsterdam}, article_number = {January}, doi = {http://dx.doi.org/10.1016/j.chroma.2013.12.039}, keywords = {Capillary electrophoresis; Contactless conductivity detection; Toxic metabolites; Methanol intoxication; Ethylene glycol intoxication; Body fluid samples}, language = {eng}, issn = {0021-9673}, journal = {Journal of Chromatography A}, title = {Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection}, url = {https://doi.org/10.1016/j.chroma.2013.12.039}, volume = {1325}, year = {2014} }
TY - JOUR ID - 1183882 AU - Kubáň, Petr - Ďurč, Pavol - Bittová, Miroslava - Foret, František PY - 2014 TI - Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection JF - Journal of Chromatography A VL - 1325 IS - January SP - 241-246 EP - 241-246 PB - Elsevier SN - 00219673 KW - Capillary electrophoresis KW - Contactless conductivity detection KW - Toxic metabolites KW - Methanol intoxication KW - Ethylene glycol intoxication KW - Body fluid samples UR - https://doi.org/10.1016/j.chroma.2013.12.039 L2 - https://doi.org/10.1016/j.chroma.2013.12.039 N2 - A new method for rapid determination of toxic metabolites after methanol and ethylene glycol intoxication - oxalate, formate and glycolate in various body fluid samples (blood serum, saliva, urine, exhaled breath condensate) by capillary electrophoresis with contactless conductometric detection was developed. A selective separation of the three target analytes from other constituents present in the analyzed biological matrices was achieved in less than 6 min in a fused silica capillary of 25 mu m I.D. using an electrolyte comprising 50 mM L-histidine and 50 mM 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The only sample preparation was dilution with deionized water. The limits of detection were 0.4, 0.6 and 1.3 mu M and limits of quantitation 1.3, 1.9 and 4.2 mu M for oxalate, formate and glycolate, respectively. The method provides a simple and rapid diagnostic test in suspected intoxication and is able to distinguish the ingested liquid, based on its metabolite trace. The method presents a fast screening tool that can be applicable in clinical practice. (C) 2013 Elsevier B.V. All rights reserved. ER -
KUBÁŇ, Petr, Pavol ĎURČ, Miroslava BITTOVÁ a František FORET. Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, 2014, roč.~1325, January, s.~241-246. ISSN~0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2013.12.039.
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