Separation of oxalate, formate and glycolate in human body
fluid samples by capillary electrophoresis with contactless
conductometric detection

J 2014

Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection

KUBÁŇ, Petr; Pavol ĎURČ; Miroslava BITTOVÁ a František FORET

Základní údaje

Originální název

Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection

Autoři

KUBÁŇ, Petr; Pavol ĎURČ; Miroslava BITTOVÁ a František FORET

Vydání

Journal of Chromatography A, Amsterdam, Elsevier, 2014, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.169

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/14:00073642

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Capillary electrophoresis; Contactless conductivity detection; Toxic metabolites; Methanol intoxication; Ethylene glycol intoxication; Body fluid samples

Štítky

AKR, podil, rivok

Změněno: 4. 10. 2019 13:22, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

A new method for rapid determination of toxic metabolites after methanol and ethylene glycol intoxication - oxalate, formate and glycolate in various body fluid samples (blood serum, saliva, urine, exhaled breath condensate) by capillary electrophoresis with contactless conductometric detection was developed. A selective separation of the three target analytes from other constituents present in the analyzed biological matrices was achieved in less than 6 min in a fused silica capillary of 25 mu m I.D. using an electrolyte comprising 50 mM L-histidine and 50 mM 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The only sample preparation was dilution with deionized water. The limits of detection were 0.4, 0.6 and 1.3 mu M and limits of quantitation 1.3, 1.9 and 4.2 mu M for oxalate, formate and glycolate, respectively. The method provides a simple and rapid diagnostic test in suspected intoxication and is able to distinguish the ingested liquid, based on its metabolite trace. The method presents a fast screening tool that can be applicable in clinical practice. (C) 2013 Elsevier B.V. All rights reserved.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GA13-21919S, projekt VaV

Název: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.

Investor: Grantová agentura ČR, Vývoj neinvazivních metod odberu vzorku a analýzy v point-of-care dignostice biologických materiálu

Přiložené soubory

ZVV_2014_258_1183882_Separation_of_oxalate.pdf

Požádat o autorskou verzi souboru

Citovat

KUBÁŇ, Petr; Pavol ĎURČ; Miroslava BITTOVÁ a František FORET. Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection. Journal of Chromatography A. Amsterdam: Elsevier, 2014, roč. 1325, January, s. 241-246. ISSN 0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2013.12.039.

@article{1183882,
   author = {Kubáň, Petr and Ďurč, Pavol and Bittová, Miroslava and Foret, František},
   article_location = {Amsterdam},
   article_number = {January},
   doi = {https://doi.org/10.1016/j.chroma.2013.12.039},
   keywords = {Capillary electrophoresis; Contactless conductivity detection; Toxic metabolites; Methanol intoxication; Ethylene glycol intoxication; Body fluid samples},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection},
   url = {https://doi.org/10.1016/j.chroma.2013.12.039},
   volume = {1325},
   year = {2014}
}

TY  - JOUR
ID  - 1183882
AU  - Kubáň, Petr - Ďurč, Pavol - Bittová, Miroslava - Foret, František
PY  - 2014
TI  - Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection
JF  - Journal of Chromatography A
VL  - 1325
IS  - January
SP  - 241-246
EP  - 241-246
PB  - Elsevier
SN  - 00219673
KW  - Capillary electrophoresis
KW  - Contactless conductivity detection
KW  - Toxic metabolites
KW  - Methanol intoxication
KW  - Ethylene glycol intoxication
KW  - Body fluid samples
UR  - https://doi.org/10.1016/j.chroma.2013.12.039
L2  - https://doi.org/10.1016/j.chroma.2013.12.039
N2  - A new method for rapid determination of toxic metabolites after methanol and ethylene glycol intoxication - oxalate, formate and glycolate in various body fluid samples (blood serum, saliva, urine, exhaled breath condensate) by capillary electrophoresis with contactless conductometric detection was developed. A selective separation of the three target analytes from other constituents present in the analyzed biological matrices was achieved in less than 6 min in a fused silica capillary of 25 mu m I.D. using an electrolyte comprising 50 mM L-histidine and 50 mM 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The only sample preparation was dilution with deionized water. The limits of detection were 0.4, 0.6 and 1.3 mu M and limits of quantitation 1.3, 1.9 and 4.2 mu M for oxalate, formate and glycolate, respectively. The method provides a simple and rapid diagnostic test in suspected intoxication and is able to distinguish the ingested liquid, based on its metabolite trace. The method presents a fast screening tool that can be applicable in clinical practice. (C) 2013 Elsevier B.V. All rights reserved.
ER  -

KUBÁŇ, Petr; Pavol ĎURČ; Miroslava BITTOVÁ a František FORET. Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, 2014, roč.~1325, January, s.~241-246. ISSN~0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2013.12.039.

Přehled o publikaci